A Microbial Sensor for Highly Sensitive and Automatic Determination of Sulfite in Foods and Food Materials. Kinetic Study of the Sulfite Dehydrogenase-catalyzed Reaction in the Microbial State of Thiobacillus thiooxidans.

Kinetic Study of the Sulfite Dehydrogenase-catalyzed Reaction in the Subcellular State of Thiobacillus thiooxidans.

“…1 Graduate School of Agriculture, The Prefecture University of Kyoto (1-5, Nakaragi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan) 2Mitsukan Group Corporation (2-6 , Nakamura-cho, Handa 475-8585, Japan) A highly sensitive and automatic sensor apparatus has been constructed with a microbe, Thiobacillus thiooxidans, and certified to be practically useful for sulfite determination in foods and food materials including starch and its products by our research group.1-7) In previous studies, 8 we made kinetic observations such as the proportionality between the initial velocity and microbe of T. thiooxidans, optimal pH, kinetic parameters, Michaelis constant (Km) and molar activity (10), and the proportionality between ferricyanide and the microbe. As a result of these examinations, the kinetic bases on the sulfite dehydrogenasecatalyzed reactions have been confirmed for the microbe.…”