A Microbial Biosensor Platform for Inline Quantification of Acetate in Anaerobic Digestion: Potential and Challenges

Kretzschmar, Jörg; Rosa, Luís F. M.; Zösel, J.; Mertig, Michael; Liebetrau, Jan; Harnisch, Falk

doi:10.1002/ceat.201500406

Cited by 32 publications

(21 citation statements)

References 29 publications

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“…S3). The upper measurement limit obtained here was higher than that found in a previous study [33]. The phenomenon of two linear ranges was also observed in a recently study using microbial electrochemical technology for monitoring the volatile fatty acids [34].…”

Section: Detection Of Organic Mattersupporting

confidence: 63%

A cathode-shared microbial fuel cell sensor array for water alert system

Jiang

Liang

et al. 2017

International Journal of Hydrogen Energy

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The use of biosensors for water alert system is critical for providing safe water to the general public. A cathode-shared microbial fuel cell (MFC) sensor array was designed improve the detection credibility by eliminating the cathode performance variation, based on the principle that only the bioanode would be used as the sensing and transducing element. Four integrated MFCs exhibited high parallelism, and independence without the cross contamination or electric signal interference. Two linear ranges were observed (from 0.25 to 0.75 mM and from 1 to 10 mM) for acetate detection. A linear relationship between inhibition ratio (IR) and Cu 2+ concentration from 2 to 6 mg/L was recorded. The sensor expressed an immediate voltage decrease when exposed to a pH decrease from 6 to 4. The compact design of the cathode-shared MFC sensor array assure the detection credibility, and the number of the integrated MFC sensors is alterable based on the monitoring requirement.

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Section: Detection Of Organic Mattersupporting

confidence: 63%

A cathode-shared microbial fuel cell sensor array for water alert system

Jiang

Liang

et al. 2017

International Journal of Hydrogen Energy

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“…All examined biofilms showed comparable maximum current densities during the last batch cycle of j max = 0.559 ± 0.025 mA cm -2 for 10 mmol L -1 acetate. This value is representative for Geobacter sp.-dominated biofilms [2,3,10,30]. Biofilm formation using 2 mmol L -1 of each acetate, propionate, and butyrate showed a maximum current density of j max = 0.478 mA cm -2 (only one test).…”

Section: Biofilm Formationmentioning

confidence: 88%

Microbial Electrochemical Sensors for Anaerobic Digestion Process Control – Performance of Electroactive Biofilms under Real Conditions

et al. 2018

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The autonomous growth of Geobacter sp.‐dominated biofilms on electrodes and their sensitivity on volatile fatty acids (VFAs) allows their application as biosensor. In this study, the implementation of a microbial electrochemical sensor in the anaerobic digestion (AD) process is reported. The amperometric sensor signal follows the acetate concentration profile in the AD process. Yet, the sensor's stability is impaired by the process conditions. Therefore, potential biofilm inhibitors are individually examined, i.e., high salt and NH4+ concentrations as well as fumarate as potential alternative electron acceptor. The results show no influence of high salinity on the signal, whereas ammonia at a concentration ≥ 5 g L−1 NH4+ acts as inhibitor, and fumarate is used as substrate for current generation.

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“…Recently, amperometric electrochemical sensor was set up for in-line measurement of acetate, using a microbial electrode based om Geobacter spp. for the determination of acetate [57]. The bacteria were shown to oxidize acetate making the electrode a terminal electron acceptor.…”

Section: Interestingly Yin Et Al Produced Poly(hydroxybutyrate-co-hmentioning

confidence: 99%

“…The organic acids that can be measured are propionate, butyrate, and other volatile fatty acids (VFA). Thus, in different settings and conditions, real-time inline monitoring of VFA concentration has been shown to be feasible [57][58][59]. Currently, VFA are measured using off-line, gas or liquid chromatography methods.…”

Section: Interestingly Yin Et Al Produced Poly(hydroxybutyrate-co-hmentioning

confidence: 99%

PHB Production in Biofermentors Assisted through Biosensor Applications

Poltronieri¹,

Mezzolla²,

D’Urso³

2017

Preprint

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Polyhydroxyalcanoates (PHAs) are biodegradable polymers synthesized in cytoplasmic granules in bacteria, such as Cupriavidus necator (Ralstonia eutropha), Alcaligenes latus, Pseudomonas spp., Comamonas spp. and other species. PHAs accumulation occurs in response to stress conditions, i.e. under high carbon and low nitrogen (24:1 ratio). PHA can be synthesized using recombinant microorganisms (provided with the operon phbA/phbB/phbC), escaping the constrains of nutrient request, except addition of high amount of sugar (glucose, lactose, fructose). In this study; E. coli was genetically modified for PHB production in biofermentors. The production of PHA at industrial scale requires a continuous supplementation of fermentable sugars to support the availability of nutrients and to assess the level of the exponential growth phase; since sugars are required either for bacterial growth either for PHA synthesis and energy storage. The biofermentors need to be run in automated system. Sensors are used at many points in fermentators; in the evaluation of parameters: consumption of sugars; cell density; quantification of PHB synthesis. The need of operational control during the fermentation has prompted us to application of three measurements; one unit linked to a Nanodrop to evaluate OD; one linked to a reaction chamber to measure sugars consumed by enzyme based fluorescence detection; and one for bacteria Nile Blue staining and fluorescence intensity reads. The growth of bacteria on three different plant by-products was monitored and PHB production in four days using a banana by-product feed was optimised. These detectors will make possible to exploit the full potential of bioreactors optimizing the time of use and maximizing the number of bacteria synthesizing PHA.

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A Microbial Biosensor Platform for Inline Quantification of Acetate in Anaerobic Digestion: Potential and Challenges

Cited by 32 publications

References 29 publications

A cathode-shared microbial fuel cell sensor array for water alert system

A cathode-shared microbial fuel cell sensor array for water alert system

Microbial Electrochemical Sensors for Anaerobic Digestion Process Control – Performance of Electroactive Biofilms under Real Conditions

PHB Production in Biofermentors Assisted through Biosensor Applications

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