A Microanalytical Method for the Analysis of Gold in Biological Media by Flameless Atomic Absorption Spectroscopy

Rp, Sharma

doi:10.1097/00007691-198206000-00011

Cited by 10 publications

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“…Samples of blood were obtained in duplicates at 0.5, 2, 4, 7, 24 hand at 2, 3,5, 7, 10 and 14days by cardiac puncture, and the concentrations of gold in the serum was measured by a flameless atomic absorption spectrophotometric method (12).…”

Section: Serum Kineticsmentioning

confidence: 99%

Role of metallothionein in cellular uptake and disposition of gold sodium thiomalate

Sharma

1984

European Journal of Drug Metabolism and Pharmacokinetics

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Rat liver and kidney tissue uptake of gold and its localization in the cytosol was studied following various doses of gold sodium thiomalate (GST). The timecourse of gold incorporation into intracellular gold-binding ligands following repeated injections of GST was also investigated (11 injections, one dose/week). Results show that between 30 and 60% of the hepatic and renal gold was localized in the cytosol over a wide range of GST doses. This was also true following repeated doses. In the kidney, the binding of gold to high molecular weight (HMW) proteins was saturated after the third GST dose, while incorporation into the metallothioneins (MT) continued to increase, accounting for as much as 50% of cytosolic gold. On the other hand the binding to hepatic MT was about 10x lower, and the proportion of cytosolic gold incorporated into the MT, decreased from 30% (after first 3 GST injections) to about 15% (following the last 3 injections). The results show that the stimulation of MT biosynthesis in different tissues as a response to the injected GST is not the same and varies within each organ with the dose and/or the duration of repeated exposure. In the liver, the ability of gold to induce MT synthesis was limited and the importance of MT in the cellular uptake and disposition of gold may largely be confined to the kidneys. It is suggested that besides playing a possible role in the detoxification of cellular gold, particularly in the kidney, MT may also contribute towards the retention and localization of gold in the tissues.

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Section: Serum Kineticsmentioning

confidence: 99%

Role of metallothionein in cellular uptake and disposition of gold sodium thiomalate

Sharma

1984

European Journal of Drug Metabolism and Pharmacokinetics

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“…The isolated cells were washed twice by resuspending in sterile isotonic saline and centrifuging as above. Sam ples of whole blood, serum, urine and blood cells were analyzed for gold by flameless atomic absorption spec trophotometry [21], Adverse reactions were docu mented and evidence of drug-associated hacmatological, hepatic or renal dysfunction was sought by appro priate laboratory tests (e.g. measurements of alkaline phosphatase, bilirubin, ESR, haemoglobin, proteinurea.…”

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confidence: 99%

Gold Concentrations and Toxicity during Oral Gold Treatment with Auranofin

1985

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The concentrations of gold in whole blood, serum, urine and blood cells of patients with rheumatoid arthritis were measured during 6 months of oral treatment either with Auranofin or placebo. Any adverse effects attributable to the treatments were also recorded. Although the time course of gold during Auranofin therapy was similar to that of injected gold, the blood and serum gold concentrations were significantly lower than those measured in patients receiving injected gold. Between 45 and 58% of Auranofin gold in the blood was associated with blood cells. In comparison, following injections of gold thiomalate, only about 4% of the gold was present in the blood cells. During the 6-month period, 2 patients receiving Auranofin withdrew because of diarrhoea and another because of rash. 1 placebo patient withdrew because of headaches. No laboratory evidence of haematological, renal or hepatic abnormality was encountered. It is suggested that the markedly lower concentrations of gold in the body sustained during treatment with Auranofin may be the critical factor towards a greater tolerance of the drug in the treatment of rheumatoid arthritis.

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1998

Atomic Absorption Spectrometry

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A Microanalytical Method for the Analysis of Gold in Biological Media by Flameless Atomic Absorption Spectroscopy

Cited by 10 publications

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Role of metallothionein in cellular uptake and disposition of gold sodium thiomalate

Role of metallothionein in cellular uptake and disposition of gold sodium thiomalate

Gold Concentrations and Toxicity during Oral Gold Treatment with Auranofin

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