A micro-rheological and rheological study of biopolymers solutions: Hyaluronic acid

Dodero, Andrea; Williams, R. L.; Gagliardi, Simona; Vicini, Silvia; Alloisio, Marina; Castellano, Maila

doi:10.1016/j.carbpol.2018.09.072

Cited by 70 publications

(57 citation statements)

References 47 publications

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“…This generalization is the basis for passive microrheology, a technique that since its inception 36 has been widely applied to study polymers, including biopolymers such as hyaluronan. 18,21 One important condition for the generalized Stokes-Einstein relation to hold is that the tracer particles are large enough compared to the correlation length of the polymer network such that they perceive the network as a viscoelastic continuum. 37 In entangled polymer solutions, the tracer particle motions will then reflect the bulk solution viscosity.…”

Section: Ddm Analysismentioning

confidence: 99%

“…A way to overcome this difficulty is to reconstitute extracellular hydrogels from purified hyaluronan. Several previous studies have indeed reported measurements of particle mobility in reconstituted hyaluronan gels based on either passive microrheology (using video tracking or light scattering to detect the thermal motions of tracer particles [18][19][20] or active microrheology (using optical tweezer manipulation of tracer particles 21 ). With few exceptions, 22,23 the focus of these studies was on single-component networks of hyaluronan networks that interact solely through excluded volume and electrostatic interactions.…”

Section: Introductionmentioning

confidence: 99%

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Particle diffusion in extracellular hydrogels

et al. 2020

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Section: Ddm Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Particle diffusion in extracellular hydrogels

et al. 2020

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“…48 For hyaluronan with a molecular weight of 1.5-1.8 MDa, c*  2 mg/ml. 49,50 We characterized the time-dependent rheology of the samples by performing frequency sweeps using strain oscillations with a small (0.5%) amplitude. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Connecting the stimuli-responsive rheology of biopolymer hydrogels to underlying hydrogen-bonding interactions

Giubertoni

Burla

Bakker

et al. 2020

Preprint

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Many biopolymer hydrogels are environmentally responsive because they are held together by physical associations that depend on pH and temperature. Here we investigate how the pH and temperature response of the rheology of hyaluronan hydrogels is connected to the underlying molecular interactions. Hyaluronan is an essential structural biopolymer in the human body with many applications in biomedicine. Using two-dimensional infrared (2DIR) spectroscopy, we show that hyaluronan chains become connected by hydrogen bonds when the pH is changed from 7.0 to 2.5, and that the bond density at pH 2.5 is independent of temperature. Temperature-dependent rheology measurements show that due to this hydrogen bonding the stress relaxation at pH 2.5 is strongly slowed down in comparison to pH 7.0, consistent with the sticky reptation model of associative polymers. From the flow activation energy we conclude that each polymer is crosslinked by multiple (5-15) hydrogen bonds to others, causing slow macroscopic stress relaxation, despite the short time scale of breaking and reformation of each individual hydrogen bond. Our findings can aid the design of stimuli-responsive hydrogels with tailored viscoelastic properties for biomedical applications.

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“…Micro-rheological measurements were carried out using a Zetasizer Nano ZS, Malvern, with a detection angle of 173 • C and a temperature of 25 • C, applying the theory reported by Dodero et al [26]. The micro-rheological characterization of 5-FU loaded freshly prepared QA-Ch or SB-Ch NP was performed using mucin from porcine stomach, Type II.…”

Section: Micro-rheological Characterization Of Np Mucoadhesive Propermentioning

confidence: 99%

“…Ocular mucins are not commercially available, so porcine gastric mucin was used because it has also been applied as a model substance in other studies, investigating ocular mucoadhesion [27]. In order to obtain reliable micro-rheological data, the conditions about the tracer-sample combination were verified as reported by Dodero et al, 2019 [26]. A dispersion of mucin 3 mg/mL in water, was filtered using a cellulose acetate filter (pore size 0.45 µm).…”

Section: Micro-rheological Characterization Of Np Mucoadhesive Propermentioning

confidence: 99%

Impact of Different Mucoadhesive Polymeric Nanoparticles Loaded in Thermosensitive Hydrogels on Transcorneal Administration of 5-Fluorouracil

et al. 2019

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In a previous paper a thermosensitive hydrogel formulation based on chitosan or its derivatives (TSOH), containing medicated chitosan nanoparticles (Ch NP) for transcorneal administration of 5-fluorouracil (5-FU) was described. The Ch NP-containing TSOH allowed a time-constant 5-FU concentration in the aqueous for 7 h from instillation. The aim of the present work was to study the impact of the surface characteristics of new NP contained in TSOH on ocular 5-FU bioavailability. The Ch derivatives used to prepare NP were quaternary ammonium-Ch conjugate (QA-Ch), S-protected derivative thereof (QA-Ch-S-pro), and a sulphobutyl chitosan derivative (SB-Ch). All NP types had 300–400 nm size, 16–18% encapsulation efficiency, and retained the entrapped drug for at least 15 h. Drug release from TSOH containing NP based on QA-Ch or QA-Ch-S-pro was virtually equal, whereas with TSOH containing NP based on SB-Ch was significantly slower. Instillation, in rabbit eyes, of NP-containing TSOH based on QA-Ch or SB-Ch led to a plateau in the aqueous concentration vs. time plot in the 1–10 h range with significantly enhanced area under curve (AUC). Negative charges on the NP surface slowed down 5-FU release from TSOH while positive charges increased NP contact with the negatively charged ocular surface. Either results in enhanced ocular bioavailability.

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A micro-rheological and rheological study of biopolymers solutions: Hyaluronic acid

Cited by 70 publications

References 47 publications

Particle diffusion in extracellular hydrogels

Particle diffusion in extracellular hydrogels

Connecting the stimuli-responsive rheology of biopolymer hydrogels to underlying hydrogen-bonding interactions

Impact of Different Mucoadhesive Polymeric Nanoparticles Loaded in Thermosensitive Hydrogels on Transcorneal Administration of 5-Fluorouracil

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