A micro-pressure sensor-based analytic platform and its application in thrombin quantification

Yang, Weiqiang; Li, Rongjie; Wang, Qingping; Wei, Qing; Chen, Fu; Lin, Zhenyu

doi:10.1039/c5ay01907a

Cited by 7 publications

(2 citation statements)

References 13 publications

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“…Furthermore, results just recorded on the paper were difficult to connecting with digital devices (i.e. iPads, computers, or phones with appropriate application programs), which emerged their increasing importance in personal health care13. In an early study, our group successfully employed a commercially available differential pressure gauge to set up an analytic method for thrombin detection13.…”

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“…iPads, computers, or phones with appropriate application programs), which emerged their increasing importance in personal health care13. In an early study, our group successfully employed a commercially available differential pressure gauge to set up an analytic method for thrombin detection13. However, it was found that catalase for catalysing the decomposition of hydrogen peroxide was inefficient and easily inactivated.…”

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confidence: 99%

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A Portable Immunosensor with Differential Pressure Gauges Readout for Alpha Fetoprotein Detection

Wang

Shao

et al. 2017

Sci Rep

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A portable, affordable and simple detector is requested in a “Point-of-Care-Testing” (POCT) system. In this study, we exploited the potentialities of Differential Pressure Gauge (DPG) to the orientation of POCT technology. Alpha fetoprotein (AFP) was chosen as a model analyte that could specifically recognized by its antigen, and a tiny outfits equipped with a DPG was employed as the signal readout. Pt/SiO2 nanospheres were synthesized and modified with the detection antibody. In the presence of target, a sandwich of immunocomplex specifically formed and the Pt/SiO2 had been modified on the capture antibody. Which then can be dissolved to release plenty of Pt and the suspensions were transferred into a closed vial filled with appropriated amount of hydrogen peroxide. Subsequently, hydrogen peroxide was decomposed to produce oxygen, resulting in the enhancement of pressure in the closed vial and which can be detected by DPG easily. Under the optimized conditions, the read out signal from DPG had a direct relationship with AFP concentrations in the range of 10~200 ng/mL, and the detection limit was as low as 3.4 ng/mL. The proposed portable sensor had been successfully applied to detect AFP in serum samples with satisfactory results. This strategy holds a great promising in biological analysis as its convenient operations, reliable results and flexible apparatus.

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confidence: 99%