A micro-method for the determination of acetaldehyde and ethanol in blood

Roach, Mary K.; Creaven, Patrick J.

doi:10.1016/0009-8981(68)90138-1

Cited by 77 publications

(23 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To evaluate the degree of intoxication in the chronic state after at least 6 mo of alcohol intake, samples for blood ethanol determinations were performed after acute ingestion on at least two occasions in each animal (41) and averaged 213+7 mg/100 ml. Alcohol administration was often associated with some degree of locomotor incoordination and occasional short periods of sleep.…”

Section: Resultsmentioning

confidence: 99%

Myocardial Function and Lipid Metabolism in the Chronic Alcoholic Animal

Regan¹,

Khan²,

Ettinger³

et al. 1974

J. Clin. Invest.

104

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Myocardial Function and Lipid Metabolism in the Chronic Alcoholic Animal

Regan¹,

Khan²,

Ettinger³

et al. 1974

J. Clin. Invest.

104

View full text Add to dashboard Cite

“…To analyze BEC, the micromethod of Roach and Creaven (1968) was used without significant modification. Samples were analyzed for ethanol by gas chromatography (model 700 Capco Anaerobic Identification system, Sunnyvale, CA).…”

Section: Determination Of Blood Ethanol Concentration (Bec)mentioning

confidence: 99%

Severity of alcohol withdrawal symptoms depends on developmental stage of Long–Evans rats

Chung

Wang

Wehman

et al. 2008

Pharmacology Biochemistry and Behavior

View full text Add to dashboard Cite

To investigate alcohol dependency and the potential role of age of initial alcohol consumption, Long-Evans (LE) rats were fed an ethanol-containing liquid diet starting at postnatal (P) ages (days): P23-27 (juvenile), P35-45 (adolescent) or P65-97 (young adult). Severity of subsequent withdrawal symptoms was dependent on age when consumption began and on duration of alcohol consumption. Frequency of withdrawal seizures was highest for rats starting consumption as juveniles, intermediate for adolescents and lowest for adults. Normalized to body weight, alcohol consumption was significantly higher for adolescent and juvenile rats than for adults. SpragueDawley rats that began alcohol consumption as adolescents (P35) had a level of alcohol consumption identical to that of the adolescent LE rats but showed much lower frequency of withdrawal seizures when tested after 2, 3 and 5 weeks of alcohol consumption. Based on several indicators, the capacity of the juveniles to metabolize ethanol is equal to or exceeds that of adults. Recoveries from a single dose of ethanol (2.5g ethanol/kg body weight) were faster for juvenile LE rats than adults. The rate of decline in blood ethanol concentration was identical for juvenile and adult rats while the corrected ethanol elimination rate was higher for juveniles. The primary isozyme of alcohol dehydrogenase (ADH) in rat liver, ADH-3, had a similar Km and higher activity in liver preparations from juveniles. In conclusion, LE rats beginning alcohol consumption as juveniles or adolescents develop a severe alcohol withdrawal syndrome that may not be attributed entirely to higher levels of consumption and was not explained by any obvious deficiencies in metabolism.

show abstract

“…Blood samples (20 μL) were assessed for ethanol content, utilizing a modification of the method originally described by Roach and Creaven (1968). The supernatant, obtained from processing the blood sample (see Finn et al, 2006), was transferred to a crimp top glass vial and analyzed by gas chromatography (Model 6890N, Agilent Technologies, Palo Alto, CA) with flame ionization detection.…”

Section: Bec Determinationmentioning

confidence: 99%

Decreased anticonvulsant efficacy of allopregnanolone during ethanol withdrawal in female Withdrawal Seizure-Prone vs. Withdrawal Seizure-Resistant mice

Beckley¹,

Fretwell²,

Tanchuck³

et al. 2008

Neuropharmacology

View full text Add to dashboard Cite

SUMMARYThe GABAergic neurosteroid allopregnanolone (ALLO) has been repeatedly shown to have an increased anticonvulsant effect during ethanol withdrawal in rats and in C57BL/6J mice. In contrast, the seizure prone DBA/2J inbred strain and the Withdrawal Seizure-Prone (WSP) selected line exhibited decreased sensitivity to ALLO's anticonvulsant effect during ethanol withdrawal, with no change in sensitivity in the Withdrawal Seizure-Resistant (WSR) line. To date, only male mice have been tested. Thus, the present study examined ALLO sensitivity during ethanol withdrawal in female WSP and WSR mice, since females display less severe physical symptoms of withdrawal and have higher circulating ALLO levels than males. Female WSP and WSR mice were exposed to ethanol vapor or air for 72 hr. During peak ethanol withdrawal, separate groups of mice were injected with vehicle or ALLO (0, 3.2, 10, or 17 mg/kg, ip) prior to the timed tail vein infusion of pentylenetetrazol (PTZ). ALLO injection significantly increased the threshold dose for onset to PTZ-induced convulsions, indicating an anticonvulsant effect, in female WSP and WSR mice. During ethanol withdrawal, sensitivity to ALLO's anticonvulsant effect was slightly increased in female WSR mice but was significantly decreased in female WSP mice. This line difference in sensitivity to ALLO during ethanol withdrawal in female mice was similar to that in the male mice. Notably, all seizure prone genotypes tested to date displayed tolerance to the anticonvulsant effect of ALLO during ethanol withdrawal, suggesting that decreased sensitivity of GABA A receptors to ALLO may contribute to the increased ethanol withdrawal phenotype.

show abstract

A micro-method for the determination of acetaldehyde and ethanol in blood

Cited by 77 publications

References 3 publications

Myocardial Function and Lipid Metabolism in the Chronic Alcoholic Animal

Myocardial Function and Lipid Metabolism in the Chronic Alcoholic Animal

Severity of alcohol withdrawal symptoms depends on developmental stage of Long–Evans rats

Decreased anticonvulsant efficacy of allopregnanolone during ethanol withdrawal in female Withdrawal Seizure-Prone vs. Withdrawal Seizure-Resistant mice

Contact Info

Product

Resources

About