A micro-method for peripheral leucocyte migration in tuberculin sensitivity

Abstract

(Dumonde, 1970). The ability of these soluble factors to inhibit the migration of cultured macrophages and leucocytes provides one measure ofcellular hypersensitivity (see Dumonde and Maini, 1971). In this paper we describe an improved method for detecting the inhibition of leucocyte migration by specific antigen which is based on the technique of Bendixen and S0borg (1969 90 min in a non-wettable plastic sterile test tube, by which time the red cells had settled at the bottom, leaving a leucocyte-rich plasma layer above. The leucocytes were centrifuged at room temperature at 150 g for 10 min, washed thrice in Waymouth's medium containing 10% horse serum, and resuspended in the medium at 7 x 107 leucocytes/ml.Micro-capillaries (20 IlI volume, Drummond microcaps2) were cut in two equal parts to give a 10 ,ul capillary size and these were filled with the leucocyte suspension and plugged at one end with wax. The 10 pl capillaries were placed in a test tube and spun at 600 g for five min; these were cut at the cell-fluid interface and were mounted on silicone dabs in the chambers. The chamber consisted of a plastic ring 16 mm diameter, 2 mm high, mounted with silicone grease on an ether-cleaned glass slide (Fig. la)

Horizontal studies of cell mediated immune reactions to autologous tumour antigens in patients with operable mammary carcinoma

“…The method used for leucocyte migration was similar to that of Federlin et al (1971) In a small number of cases, parallel studies were performed using a guinea-pig macrophage migration test (MMT) similar to that employed by Rajapakse and Glynn (1970). Guinea-pig macrophages were obtained as peritoneal exudates 3 days after the intraperitoneal injection of 20 ml sterile liquid paraffin, and were washed 3 times in medium 199.…”

Macrophage Migration Stimulation Factor

“…Soborg [55] claimed that stimulation might be considered to be very similar to inhibition, in that it reflects weak sensitization. Other workers, using peripheral blood leucocyte migration, have concluded that stimulation reflects a state of delayed hypersensitivity [12,18,54]. This phenomenon is also described when using guinea-pig peritoneal exudate cells as target.…”

“…S0borg [55] used leucocyte migration as an indication of delayed hypersensitivity, and together with others [12,18,54] accepts that stimulation of migration represents weak sensitization. This would fit in with the concept of active suppression and the production of M.St.F.…”

Dissociation of Correlates of Cellular Immunity in Man: Functional Heterogeneity within the Antigen-Reactive Cell Population?

“…Furthermore, a range of nega tive and positive results were obtained in both these tests on each occa sion and neither test showed an overall depression on any day. It should also be noted that the PPD concentrations used here for LMI are lower than those employed by some authors [27][28][29]. However, the concentra tion range employed in this study was chosen after exhaustive preliminary experiments over a wider concentration range and were shown to elicit maximal responses in the majority of subjects.…”