A method toward constituents with weak response in mass spectra for comprehensively characterizing constituents in traditional Chinese medicine formula, Kangfuxiaoyanshuan as a case

Abstract

How to identify the traces with weak response and poor mass spectrometry data is still a barrier to comprehensively characterize chemical constituents of traditional Chinese medicine formula. Thus, we took Kangfuxiaoyanshuan as a carrier to perform a method toward these weak response constituents in mass spectra. Chemical constituents profiling spectra of each herb and formula were firstly obtained by an ultra‐high‐performance liquid chromatography coupled with linear ion trap‐Orbitrap mass spectrometry. Next, the high response constituents in the formula were identified and suspicious constituents with weak response were classified preliminarily according to the reflection of chemical components from each herb. In order to clarify the suspicious, a method increasing detection concentration, optimizing chromatographic separation conditions, and online parameters in mass was established. As a result, a total of 123 chemical components including 43 suspicious components in Kangfuxiaoyanshuan were classified and characterized unambiguously.

Caffeoylquinic acids: chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity

“…Lonicerae japonicae , buds; Achyrocline satureioides (Lam) DC., aerial parts; Anthemis tinctoria , aerial parts; kangfuxiaoyanshuan; Aster tataricus L., roots and rhizomes; Angelicae Pubescentis Radix, roots; Valeriana jatamansi Jones, roots and rhizomes; Helichrysum arenarium (L.), flowers (Ma et al., 2018; Martinez‐Busi et al., 2019; Orlando et al., 2019; Rho et al., 2020; Wang, Yao, et al., 2021; Xie et al., 2019; Yang, Hou, et al., 2020; Yang, Liu, et al., 2020; Yang et al., 2021)…”

Kangfuxiaoyanshuan alleviates uterine inflammation and adhesion via inhibiting NF-κB p65 and TGF-β/MMP-2 signaling pathway in pelvic inflammatory disease rats

“…f. ]; Lithospermi radix [ Boraginaceae ; Lithospermum erythrorhizon Siebold&Zucc ] and Suis Fellis Pulvis . The various chemical components of the compound, which include alkaloids, flavonoids, diterpene lactones, bile acids, anthraquinones, naphthoquinones, and organic acids, were identified in previous studies ( C. P. Commission, 2015 ; Xie et al, 2019 ). Importantly, pharmacological studies on KFXYS have confirmed its effect in clearing heat, detoxifying, destroying parasites, and relieving itching.…”

“…Due to the disadvantages of antibiotics and surgical treatment, Traditional Chinese Medicine (TCM) formulations, which can effectively alleviate clinical symptoms, reduce the use of antibacterial drugs and shorten the course of the disease, have become the focus of research on the treatment of PID sequelae and chronic pelvic inflammatory disease (CPID) (Bu et al, 2015;Zhang et al, 2017) Commission, 2015;Xie et al, 2019). Importantly, pharmacological studies on KFXYS have confirmed its effect in clearing heat, detoxifying, destroying parasites, and relieving itching.…”

Editorial: Medicinal plants as a source of novel autoimmune-modulating and anti-inflammatory drug products

“…Kangfuxiaoyanshuan (KFXYS) is a traditional Chinese herbal compound that composed of eight classical medicinal herbs. The various chemical components of this Chinese herbal compound, including alkaloids, flavonoids, diterpenoid lactones, bile acids, anthraquinones, naphthoquinones, and organic acids ( Xie et al, 2019 ). Here, Liu et al demonstrate KFXYS remarkablely reduced the levels of proinflammatory factors (IFN-31γ, IL-1β, IL-4) and adhesion related factors (TNF-α) in the acute stage of pelvic inflammatory disease (PID), and had protective effect of ultrastructure of endometrial epithelial cells.…”