A method to study the hemodynamics of chicken embryo's aortic arches using optical coherence tomography

Ko, Zhen Yu Gordon; Mehta, Kalpesh; Jamil, Muhammad; Yap, Choon Hwai; Chen, Nanguang

doi:10.1002/jbio.201600119

Cited by 5 publications

(6 citation statements)

References 47 publications

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“…Biomechanical OCT imaging of the embryo has largely focused on the heart, including both elasticity assessment [34,[70][71][72] and shear stress analysis [63,66,67,[73][74][75]. High-resolution imaging and measurement of elasticity with OCT is an emerging area that can potentially bring a novel mechanical understanding of tissues and cells at a new scale which was previously not available [76,77].…”

Section: Optical Coherence Tomography and Microscopymentioning

confidence: 99%

“…Notably, 4D mapping of shear stress in the quail embryonic heart was achieved by Peterson et al [73], which provides dynamic visualizations and understanding of spatiotemporal variations of shear force applied to the beating embryonic heart. Computational fluid dynamics were also employed to produce mapping of the heart wall shear stress [67,75]. OCT-based shear Reproduced from [18].…”

Section: Optical Coherence Tomography and Microscopymentioning

confidence: 99%

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Label-free optical imaging in developmental biology [Invited]

Wang

Larina

Larin

2020

Biomed. Opt. Express

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Application of optical imaging in developmental biology marks an exciting frontier in biomedical optics. Optical resolution and imaging depth allow for investigation of growing embryos at subcellular, cellular, and whole organism levels, while the complexity and variety of embryonic processes set multiple challenges stimulating the development of various live dynamic embryonic imaging approaches. Among other optical methods, label-free optical techniques attract an increasing interest as they allow investigation of developmental mechanisms without application of exogenous markers or fluorescent reporters. There has been a boost in development of label-free optical imaging techniques for studying embryonic development in animal models over the last decade, which revealed new information about early development and created new areas for investigation. Here, we review the recent progress in label-free optical embryonic imaging, discuss specific applications, and comment on future developments at the interface of photonics, engineering, and developmental biology.

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Section: Optical Coherence Tomography and Microscopymentioning

confidence: 99%

Section: Optical Coherence Tomography and Microscopymentioning

confidence: 99%

Label-free optical imaging in developmental biology [Invited]

Wang

Larina

Larin

2020

Biomed. Opt. Express

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“…The use of computational modeling and numerical analysis [66] will further strengthen and elevate the spatiotemporal scale provided by OCT for cardiac biomechanical studies. Particularly, OCT has been combined with computational fluid dynamics to study the hemodynamics of the embryonic aortic arch [96]. Imaging-based computational analyses described the transitions of aortic arch patterning and hemodynamics in the early chick embryos at a number of developmental stages [97][98][99].…”

Section: Perspective On Future Developmentsmentioning

confidence: 99%

Embryonic Mouse Cardiodynamic OCT Imaging

Lopez

Wang

2020

JCDD

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The embryonic heart is an active and developing organ. Genetic studies in mouse models have generated great insight into normal heart development and congenital heart defects, and suggest mechanical forces such as heart contraction and blood flow to be implicated in cardiogenesis and disease. To explore this relationship and investigate the interplay between biomechanical forces and cardiac development, live dynamic cardiac imaging is essential. Cardiodynamic imaging with optical coherence tomography (OCT) is proving to be a unique approach to functional analysis of the embryonic mouse heart. Its compatibility with live culture systems, reagent-free contrast, cellular level resolution, and millimeter scale imaging depth make it capable of imaging the heart volumetrically and providing spatially resolved information on heart wall dynamics and blood flow. Here, we review the progress made in mouse embryonic cardiodynamic imaging with OCT, highlighting leaps in technology to overcome limitations in resolution and acquisition speed. We describe state-of-the-art functional OCT methods such as Doppler OCT and OCT angiography for blood flow imaging and quantification in the beating heart. As OCT is a continuously developing technology, we provide insight into the future developments of this area, toward the investigation of normal cardiogenesis and congenital heart defects.

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“…Such 3D models allow to take into account the fine structure of real tissues and changes in their physical parameters. The joint use of image processing techniques and numerical modeling methods such as computational fluid dynamics or heat transfer is a modern trend in the development of medicine and applied biology [13].…”

Section: Examples Of Images Reconstruction In Arbitrary Sectionsmentioning

confidence: 99%

Software for Full-Color 3D Reconstruction of the Biological Tissues Internal Structure

Хоперсков¹,

Kovalev²,

Astakhov³

et al. 2017

Health Information Science

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Abstract.A software for processing sets of full-color images of biological tissue histological sections is developed. We used histological sections obtained by the method of high-precision layer-by-layer grinding of frozen biological tissues. The software allows restoring the image of the tissue for an arbitrary cross-section of the tissue sample. Thus, our method is designed to create a full-color 3D reconstruction of the biological tissue structure. The resolution of 3D reconstruction is determined by the quality of the initial histological sections. The newly developed technology available to us provides a resolution of up to 5 -10 µm in three dimensions.

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A method to study the hemodynamics of chicken embryo's aortic arches using optical coherence tomography

Cited by 5 publications

References 47 publications

Label-free optical imaging in developmental biology [Invited]

Label-free optical imaging in developmental biology [Invited]

Embryonic Mouse Cardiodynamic OCT Imaging

Software for Full-Color 3D Reconstruction of the Biological Tissues Internal Structure

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