A Method to Separate Lignoids fromVirolaLeaves

Fernandes, Anna Maria A. P.; Prado, Aldebaran L.; Barata, Lauro Euclides Soares; Paulo, Marçal Q.; Azevedo, Neucírio Ricardo de; Ferri, Pedro Henrique

doi:10.1002/(sici)1099-1565(199701)8:1<18::aid-pca330>3.0.co;2-b

Cited by 11 publications

(3 citation statements)

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“…Dried and powdered adult leaves (445 g) of P. hemmendorffii were extracted with EtOAc (3 × 1000 mL) yielding a crude extract (27.45 g). Ten grams of extract were suspended in MeOH:H 2 O (250 mL, 85:15) and dechlorophylated in a bed of Celite [62] and extracted with hexanes and then with dichloromethane, yielding 4.51 g and 1.91 g of respective fractions. From the dichloromethane fraction, 1.5 g was submitted to vacuum liquid chromatography [63] using hexane:EtOAc at increasing polarities yielding a sample containing compound 9 (12 mg), which was analyzed by spectroscopic techniques without further purifications [35].…”

Section: Isolation Of Compounds From Piper Hemmendorffiimentioning

confidence: 99%

Ontogenetic Changes in the Chemical Profiles of Piper Species

Gaia

Yamaguchi

Guerrero-Perilla

et al. 2021

Plants

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The chemical composition of seedlings and adult plants of several Piper species were analyzed by 1H NMR spectroscopy combined with principal component analysis (PCA) and HPLC-DAD, HPLC-HRESIMS and GC-MS data. The chromatographic profile of crude extracts from leaves of Piper species showed remarkable differences between seedlings and adult plants. Adult leaves of P. regnellii accumulate dihydrobenzofuran neolignans, P. solmsianum contain tetrahydrofuran lignans, and prenylated benzoic acids are found in adult leaves of P. hemmendorffii and P. caldense. Seedlings produced an entirely different collection of compounds. Piper gaudichaudianum and P. solmsianum seedlings contain the phenylpropanoid dillapiole. Piper regnellii and P. hemmendorffii produce another phenylpropanoid, apiol, while isoasarone is found in P. caldense. Piper richadiaefolium and P. permucronatum contain dibenzylbutyrolactones lignans or flavonoids in adult leaves. Seedlings of P. richardiaefolium produce multiple amides, while P. permucronatum seedlings contain a new long chain ester. Piper tuberculatum, P. reticulatum and P. amalago produce amides, and their chemistry changes less during ontogeny. The chemical variation we documented opens questions about changes in herbivore pressure across ontogeny.

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Section: Isolation Of Compounds From Piper Hemmendorffiimentioning

confidence: 99%

Ontogenetic Changes in the Chemical Profiles of Piper Species

Gaia

Yamaguchi

Guerrero-Perilla

et al. 2021

Plants

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“…141 Purification of total extracts with lignan content is quite time-consuming and laborious. Methanol extracts are usually concentrated, diluted with water, this suspension fractioned with n-hexane and consequently with chloroform, 142 dichloromethane 143,144 or ethyl acetate, 145 to obtain a lignan fraction. For example, syringaresinol (23) from the crude extract of M. thailandica was obtained as follows: crude extract was subjected to silica gel column chromatography with ethyl acetate-hexane and methanol-ethyl acetate mixtures to give seven fractions.…”

Section: Isolation Of Lignansmentioning

confidence: 99%

Furofuran lignans of Artemisia genus: Isolation, biosynthesis and biological activity

Ickovski

Pavlović

Palić

et al. 2020

JSCS

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Since ancient times, medicinal plants and pharmacologically active products obtained from different natural sources play an important role in human health. Plants belonging to the genus Artemisia possess a great biological potential and it is a well-studied genus in the fields such as systematics (including molecular phylogenetics) and genome organization. Many species of the genus (e.g., A. absinthium, A. annua, A. vulgaris, A. abrotanum, A. arborescens) are widely exploited, because of their high economic value as medicines, food and ornamentals. Withal, in such a large genus, some hiatus must inevitably exist, concerning attainments and potentials that individual species possess. Most of the studies are focused on bioactivity and pharmacology of sesquiterpene lactones. Lignans are unjustly neglected, even though they as well exhibit a wide range of bioactivities. Motivated by that fact, we tried to consolidate findings on bioactive lignans accumulated through the years, with the logical perspectives on further work on isolation and identification of new bioactive lignans and the exploitation of lignans as substances of potential pharmacological interest.

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“…Methanolic extract can be directly analysed or concentrated and diluted with water. Methanol-water solution is further fractionated with hexane to remove nonpolar compounds including lipids, terpenes and chlorophylls and subsequently extracted with chloroform, dichloromethane [44,45] or ethyl acetate [42] to obtain the lignan fraction. The methodology using 95% ethanol seems to be better for screening plant leaves for lignans [44].…”

Section: Solvent Extractionmentioning

confidence: 99%