A Method To Disperse Aggregates of a Flocculent Yeast for Photometric Analysis

Castellon-Vogel, Martha A.; Menawat, Anil S.

doi:10.1021/bp00002a007

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…This fact did not compromise our data analysis. According to Castellon‐Vogel and Menawat (2008), aggregation of yeasts is a problem in using turbidimetric methods. Jin and Speers (1998) explained that it can occur because of many factors, such as lectin‐like cell–cell interactions, electrostatic interactions of the fungal components and the presence of cell wall proteins.…”

Section: Discussionmentioning

confidence: 99%

Ciclopirox olamine: an antifungal alternative against cryptococcosis

Oliveira

Medeiros

Macêdo

et al. 2010

Letters in Applied Microbiology

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Aims: The in vitro activity of ciclopirox olamine was evaluated against Cryptococcus spp. obtained from the cerebrospinal fluid (CSF) of immunocompromised patients. Methods and Results: The antifungal activity of ciclopirox olamine was tested against Cryptococcus spp. obtained from the CSF of immunocompromised patients, using amphotericin B and fluconazole as controls. The minimal inhibitory concentration was determined following the microdilution method indicated by the Clinical and Laboratory Standards Institute. The minimal fungicide concentration was determined by the absence of growth on Sabouraud dextrose agar. The data obtained showed that antifungal activity of ciclopirox olamine ranged from 0·25 to 1 μg ml−1. Conclusions: This paper underscores the importance of the antifungal potential of ciclopirox olamine against Cryptococcus spp. as an alternative treatment against systemic cryptococosis. In vivo experiments are essential for future medical use. Significance and Impact of the Study: This was the first time that ciclopirox olamine was tested against Cryptococcus spp. using the reference method. The antifungal activity of this drug against this species suggests an applicable potential for systemic cryptococcosis therapy.

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Section: Discussionmentioning

confidence: 99%

Ciclopirox olamine: an antifungal alternative against cryptococcosis

Oliveira

Medeiros

Macêdo

et al. 2010

Letters in Applied Microbiology

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“…The cultures were grown for 3 days at 22˚C, washed, diluted, and spread onto SC-Ura, and SC-His-Ura (for Ty1his3-AI) or YEPD + Geneticin (G418; 200 μg/ml for Ty1neo-AI) (ThermoFisher, Waltham MA) plates. To minimize flocculence, 0.25 M NaCl was used for cell washes and dilutions prior to plating [80]. The frequency of Ty1 mobility is defined as the number His + Ura + or G418 R colonies divided by the number of Ura + colonies per ml of culture.…”

Section: Ty1 Mobilitymentioning

confidence: 99%

Evolution of Ty1 copy number control in yeast by horizontal transfer and recombination

et al. 2020

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“…The cultures were grown for 3 days at 22°C, washed, diluted, and spread onto SC-Ura, and SC-His-Ura (for Ty1 his3-AI ) or YEPD + Geneticin (G418; 200 μg/ml for Ty1 neo-AI ) (ThermoFisher, Waltham MA) plates. To minimize flocculence, 0.25 M NaCl was used for cell washes and dilutions prior to plating (Castellon-Vogel and Menawat 1990). The frequency of Ty1 mobility is defined as the number His + Ura + or G418 R colonies divided by the number of Ura + colonies per ml of culture.…”

Section: Methodsmentioning

confidence: 99%

Evolution of Ty1 copy number control in yeast by horizontal transfer of agaggene

Czaja

Bensasson

Ahn

et al. 2019

Preprint

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Insertion of mobile DNA sequences typically has deleterious effects on host fitness, and thus diverse mechanisms have evolved to control mobile element proliferation across the tree of life. Mobility of the Ty1 retrotransposon in Saccharomyces yeasts is regulated by a novel form of copy number control (CNC) mediated by a self-encoded restriction factor derived from the Ty1 gag capsid gene that inhibits virus-like particle function. Here, we survey a panel of wild and human-associated strains of S. cerevisiae and S. paradoxus to investigate how genomic Ty1 content influences variation in Ty1 mobility. We observe high levels of mobility for a canonical Ty1 tester element in permissive strains that either lack full-length Ty1 elements or only contain full-length copies of the Ty1’ subfamily that have a divergent gag sequence. In contrast, low levels of canonical Ty1 mobility are observed in restrictive strains carrying full-length Ty1 elements containing canonical gag. Phylogenomic analysis of full-length Ty1 elements revealed that Ty1’ is the ancestral subfamily present in wild strains of S. cerevisiae, and that canonical Ty1 in S. cerevisiae is a derived subfamily that acquired gag from S. paradoxus by horizontal transfer and recombination. Our results provide evidence that variation in the ability of S. cerevisiae and S. paradoxus strains to repress canonical Ty1 transposition via CNC is encoded by the genomic content of different Ty1 subfamilies, and that self-encoded forms of transposon control can spread across species boundaries by horizontal transfer.

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A Method To Disperse Aggregates of a Flocculent Yeast for Photometric Analysis

Cited by 3 publications

References 15 publications

Ciclopirox olamine: an antifungal alternative against cryptococcosis

Ciclopirox olamine: an antifungal alternative against cryptococcosis

Evolution of Ty1 copy number control in yeast by horizontal transfer and recombination

Evolution of Ty1 copy number control in yeast by horizontal transfer of agaggene

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