A method to determine reactive oxygen species production in intestinal and liver cell cultures using the 2′,7′-dichlorodihydrofluorescein diacetate assay

Engelbrecht, Ilzé; Horn, Suranie; Giesy, John P.; Pieters, Rialet

doi:10.1016/j.mex.2024.102615

MethodsX

2024

DOI: 10.1016/j.mex.2024.102615

|View full text |Cite

A method to determine reactive oxygen species production in intestinal and liver cell cultures using the 2′,7′-dichlorodihydrofluorescein diacetate assay

Ilzé Engelbrecht,

Suranie Horn,

John P. Giesy

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 8 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Oxidative stress-mediated epigenetic remodeling, metastatic progression and cell signaling in cancer

Phull,

Arain,

Majid

et al. 2024

Oncologie

View full text Add to dashboard Cite

Cancer is a serious public health issue and cases are rising at a high rate around the world. Altered production of reactive oxygen species (ROS) causes oxidative stress (OS) which plays a vital role in cancer development by disrupting signaling pathways and genomic integrity in the cellular microenvironment. In this study, we reviewed the regulation of noncoding RNAs, histone modifications, and DNA methylation which OS is involved in. These mechanisms promote cancer growth, metastasis, and resistance to chemotherapeutic agents. There is significant potential to improve patient outcomes through the development of customized medications and interventions that precisely address the role of OS in the onset and progression of cancer. Redox-modulating drugs, antioxidant-based therapies, and measures to restore regular cellular activity and OS-modulated signaling pathways are some examples of these strategies. One other hypothesis rationalizes the cancer-suppressing effect of OS, which acts as a two-edged condition that warns against the use of antioxidants for cancer treatment and management. The present study was executed to review the impact of OS on epigenetic machinery, the evolution of metastatic cancer, and how OS mediates cellular signaling. Along with, insights into the potential of targeting OS-mediated mechanisms for cancer therapy.

show abstract

Oxidative stress-mediated epigenetic remodeling, metastatic progression and cell signaling in cancer

Phull,

Arain,

Majid

et al. 2024

Oncologie

View full text Add to dashboard Cite

show abstract

Radiation Therapy of Cervical Cancer Patients: View of Radiobiologists

Domina,

Dumanskyi,

Mаkovetska

et al. 2024

Oncology

View full text Add to dashboard Cite

Summary. Despite the positive results achieved in recent years in the treatment of cervical cancer (CC), the overall and recurrence-free survival of patients with this pathology leaves much to be desired. This is connected with a number of problems. The main ones are late diagnosis, which leads to the neglect of the tumor process, insufficient effectiveness of conservative treatment methods, which depends on relatively low sensitivity to the drugs used, and low specificity of the radiation therapy (RT) used. Based on the analysis of data from the literature and our own research, the way to determine among the main radiobiological indicators, which take into account the mechanisms and stages of the formation of radiation lesions, predictors of complications due to RT in CC patients. This will make it possible to single out a group at increased risk of developing negative effects of RT, to develop effective means of pathogenetic therapy for tissue damage from around the tumor, and thus to reduce the frequency, nature and degree of severity of remote side complications of RT in this category of patients, which will contribute to improving the quality of life of patients.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A method to determine reactive oxygen species production in intestinal and liver cell cultures using the 2′,7′-dichlorodihydrofluorescein diacetate assay

Cited by 2 publications

References 8 publications

Oxidative stress-mediated epigenetic remodeling, metastatic progression and cell signaling in cancer

Oxidative stress-mediated epigenetic remodeling, metastatic progression and cell signaling in cancer

Radiation Therapy of Cervical Cancer Patients: View of Radiobiologists

Contact Info

Product

Resources

About