“…Limited blood perfusion experiments have been performed in the early 1960s and 1970s, investigating mainly physiologic aspects of limb salvage in amputated human specimen [30][31][32]. The use of hemoglobin was thereafter abandoned and extremities were washed out [33,34], stored [35][36][37], perfused intermittently [34,38] or continuously [38][39][40] with fluids of different compositions, including physiological saline [41], Ringer Lactate [39], compositions using mannitol [42], and the standard organ preservation solutions University of Wisconsin [34,35,40,[43][44][45] and Euro Collins [33,39,43,45].…”