A method of preparing highly vacuolated, senescent, or damaged plant tissue for ultrastructural study

Mohr, W.; Cocking, E. C.

doi:10.1016/s0022-5320(67)80089-3

Cited by 58 publications

(13 citation statements)

References 6 publications

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“…Although gross anatomical features of a tissue rather than the fine structural components of its cells probably account for major textural properties, the possible role played by cellular membranes and other subcellular structures cannot be properly assessed until more work at the ultrastructural level has been carried out in this applied area of research. The relatively recent introduction of improved fixatives (Sabatini el al., 1963 ;Gunning, 1965) and of embedding and staining methods more suitable for preparing highly vacuolated plant tissues for electron microscopy (Mohr and Cocking, 1968 ;Spurr, 1969) now permit fine structural details of freeze-thaw damaged protoplasm to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Freeze‐thaw Damage to Protoplasmic Structure in High Moisture, Edible Plant Tissues

Mohr

1971

Journal of Texture Studies

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Three high moisture plant tissues, whose textural response to freeze-thawing differs noticeably, were studied ultrastructurally before and after controlled freeze-thaw regimes. Fine structural components of the protoplasm were usually disrupted extensively, although to varying degrees, depending on tissue type and freeze-thaw conditions. Possible reasons for the apparent protection in some of the material studied are discussed. It is concluded that protoplasmic structure probably plays an insignificant role in influencing the textural properties of high moisture, frozen-thawed plant tissues.

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Section: Introductionmentioning

confidence: 99%

Freeze‐thaw Damage to Protoplasmic Structure in High Moisture, Edible Plant Tissues

Mohr

1971

Journal of Texture Studies

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“…Tn the electron microscopic studies the method of MOHR et al (1968) was followed with slight modifications. An LKB ultramicrotom and a Japan Electron Optics Laboratory ultra microscope (Jem T7) was used to examine healthy and diseased tomato fruits (diameter I-2 cm).…”

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confidence: 99%

Investigations upon Ca related Physiological Disorders

Bangerth

1973

J Phytopathol

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“…Frond tissue was fixed witb glutaraldebye-osmium and embedded in plastic, either as previously described (Peters and Mayne, 1974a;Peters, 1976) or according to tbe procedure of Mohr and Cocking (1968). Sections were stained for various times witb 0.5% Toluidine Blue 0 in 1% sodium borate, and pbotograpbed as above or witb a Leitz Ortbolux II.…”

Section: Light Microscopy-sectioned Tissuementioning

confidence: 99%

THE AZOLLA‐ANABAENA AZOLLAE RELATIONSHIP

et al. 1978

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SUMMARYThe nitrogen-fixing blue-green alga, Anabaena azollae, occurs as a symbiont in leaf cavities of the water fern, Azolla. Organization and morphological aspects of tbe association, with empbasis upon the cavity area, were studied by light, transmission, and scanning electron microscopy. Cleared whole mounts, sectioned, and enzymatically digested materials were employed. Leaf cavity formation, including the association of the symbiont and epidermal hairs, is shown during leaf development in A. caroliniana Willd. Enzymatic digestion of the fern leaf tissue provided preparations of algal packets whicb correspond to the leaf cavity area. The digestion process complemented otber approaches in providing insigbt into the organization of the leaf cavity. Scanning electron microscopy allowed visualization of these packets in three dimensions. The packets are surrounded by a filmy, limiting envelope of unknown composition. The envelope was isolated and sbown to remain associated with hairs which protrude into the cavity area. The hairs are multicellular and may be branched or unbranched. When nitrogen fixation by the symbiont is the sole nitrogen source, sections through some cells of hairs in mature leaf cavities exhibit the ultrastructural characteristics of transfer cells. These characteristics are not observed, however, in sections through cells of hairs associated with the early stages of leaf cavity formation in tbe shoot tip. The potential significance of the envelope and hair cells in the metabolic interaction of the host and symbiont are discussed.

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A method of preparing highly vacuolated, senescent, or damaged plant tissue for ultrastructural study

Cited by 58 publications

References 6 publications

Freeze‐thaw Damage to Protoplasmic Structure in High Moisture, Edible Plant Tissues

Freeze‐thaw Damage to Protoplasmic Structure in High Moisture, Edible Plant Tissues

Investigations upon Ca related Physiological Disorders

THE AZOLLA‐ANABAENA AZOLLAE RELATIONSHIP

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