A method of packaging molecule/cell-patterns in an open space into a glass microfluidic channel by combining pressure-based low/room temperature bonding and fluorosilane patterning

Funano, Shun‐ichi; Ota, Noriyasu; Sato, Asako; Tanaka, Yo

doi:10.1039/c7cc04744d

Cited by 15 publications

(18 citation statements)

References 38 publications

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“…The glass microfluidic device was fabricated by the previously described method [39] with slight modifications. In short, the glass plates were cleaned with H 2 SO 4 /H 2 O 2 solution for 15 min to subsequently deposit Cr and Au layers on the glass surface by sputtering (EIS-220, Elionix, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Enhancement in acoustic focusing of micro and nanoparticles by thinning a microfluidic device

et al. 2019

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The manipulation of micro/nanoparticles has become increasingly important in biological and industrial fields. As a non-contact method for particle manipulation, acoustic focusing has been applied in sorting, enrichment and analysis of particles with microfluidic devices. Although the frequency and amplitude of acoustic waves and the dimensions of microchannels have been recognized as important parameters for acoustic focusing, the thickness of microfluidic devices has not been considered so far. Here, we report that thin glass microfluidic devices enhance acoustic focusing of micro/nanoparticles. It was found that the thickness of a microfluidic device strongly influences its ability to focus particles via acoustic radiation, because the energy propagation of acoustic waves is affected by the total mass of the device. Acoustic focusing of submicrometre polystyrene beads and Escherichia coli as well as enrichment of polystyrene beads were achieved in glass microfluidic devices as thin as 0.4 mm. Modifying the thickness of a microfluidic device can thus serve as a critical parameter for acoustic focusing when conventional parameters to achieve this effect are kept unchanged. Thus, our findings enable new approaches to the design of novel microfluidic devices.

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Section: Methodsmentioning

confidence: 99%

Enhancement in acoustic focusing of micro and nanoparticles by thinning a microfluidic device

et al. 2019

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“…The microchip components were aligned and stacked into the desired spatial configuration. All components were bonded with vacuum oxygen plasma 29 at an intensity of 15 W and an oxygen flow rate of 5 mL/min for 0.5 min in the chamber of a compact etcher (FA-1, SAMCO, Kyoto, Japan) ( Fig. 2a).…”

Section: Preparation Of Hmdsmentioning

confidence: 99%

Establishment of a heart-on-a-chip microdevice based on human iPS cells for the evaluation of human heart tissue function

Abulaiti

Yalikun

Murata

et al. 2020

Sci Rep

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Human iPS cell (iPSC)-derived cardiomyocytes (CMs) hold promise for drug discovery for heart diseases and cardiac toxicity tests. To utilize human iPSC-derived CMs, the establishment of three-dimensional (3D) heart tissues from iPSC-derived CMs and other heart cells, and a sensitive bioassay system to depict physiological heart function are anticipated. We have developed a heart-on-a-chip microdevice (HMD) as a novel system consisting of dynamic culture-based 3D cardiac microtissues derived from human iPSCs and microelectromechanical system (MEMS)-based microfluidic chips. The HMDs could visualize the kinetics of cardiac microtissue pulsations by monitoring particle displacement, which enabled us to quantify the physiological parameters, including fluidic output, pressure, and force. The HMDs demonstrated a strong correlation between particle displacement and the frequency of external electrical stimulation. The transition patterns were validated by a previously reported versatile video-based system to evaluate contractile function. The patterns are also consistent with oscillations of intracellular calcium ion concentration of CMs, which is a fundamental biological component of CM contraction. The HMDs showed a pharmacological response to isoproterenol, a β-adrenoceptor agonist, that resulted in a strong correlation between beating rate and particle displacement. Thus, we have validated the basic performance of HMDs as a resource for human iPSC-based pharmacological investigations.

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“…To address these issues, a bonding method was developed in which two glass plates with grooves were bonded together after multiple types of substances, proteins or cells were immobilized on fine grooves (Funano, et al ). The bonding of glass plates was realized only by cleaning the surface and pressing the glass plates at room temperature combined with the fluoro‐functional group patterning method described in the previous chapter.…”

Section: Molecule and Cell Patterning Into A Glass Microchannelmentioning

confidence: 99%

“…White and black bars indicate 1 cm and 500 μm, respectively. All panels are adapted from figures presented in Funano, Ota, et al ()…”

Section: Molecule and Cell Patterning Into A Glass Microchannelmentioning

confidence: 99%

User‐friendly cell patterning methods using a polydimethylsiloxane mold with microchannels

Funano

Tanaka

2019

Dev Growth Differ

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Techniques for partitioning cell adhesion are useful tools in biological and medical experiments. However, conventional cell patterning methods require special apparatus, special materials or high‐level skills. Therefore, we have developed a new cell patterning methodology which can be easily carried out in biological laboratories. Non‐cell adhesive material including hydrogel or gas patterns to restrict cell adhesion on a culture dish or glass substrates can be constructed by exploiting a polydimethylsiloxane (PDMS) mold with microchannels. The PDMS molds suck non‐adhesive materials into microchannels from the inlet of the microchannels and the materials are immobilized onto the substrates with a desired pattern. High resolution under a few micrometers and long‐term stability can be realized. This method has been used for analysis of stem cells, muscle cells, neuron development and other cells in collaboration with many biological researchers. Several examples to use this technique are introduced in this review.

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A method of packaging molecule/cell-patterns in an open space into a glass microfluidic channel by combining pressure-based low/room temperature bonding and fluorosilane patterning

Cited by 15 publications

References 38 publications

Enhancement in acoustic focusing of micro and nanoparticles by thinning a microfluidic device

Enhancement in acoustic focusing of micro and nanoparticles by thinning a microfluidic device

Establishment of a heart-on-a-chip microdevice based on human iPS cells for the evaluation of human heart tissue function

User‐friendly cell patterning methods using a polydimethylsiloxane mold with microchannels

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