A Method of Enumeration of Individual Marrow Elements for Research Purposes

Observations on the Behaviour of Erythroblasts Cultured in Normal and "Pernicious Anaemia" Sera

“…A preliminary step was to devise a method of counting one element of a bone-marrow suspension with a known degree of accuracy; the method adopted is dscribed elsewhere (Sharp, Feinmann, and Wilkinson, 1952), together with a suggested modification, use of which will lessen counting labour. Briefly, an estimate of known accuracy of the count of the total nucleated cells per unit volume is made; the estimate of the proportion that the marrow element forms of the total nucleated cells, also of known accuracy; is derived from differential counts on a series of cover-slip smears.…”