A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts

Shatil, Anwar S.; Matsuda, Kyle; Figley, Chase R.

doi:10.3389/fneur.2016.00208

Cited by 40 publications

(36 citation statements)

References 38 publications

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“…The richness of knowledge provided by these resources could be substantially amplified by post-mortem imaging studies, which would allow correlation between the gold standard of neuropathology and the findings of so-called virtual autopsies 370 based on advanced and tailored MRI techniques. 371,372 Finally, these precious archive resources must be networked and made widely accessible to be suitable for international collaborative research.…”

Section: Brain Banks and Lessons From Neuropathologymentioning

confidence: 99%

Traumatic brain injury: integrated approaches to improve prevention, clinical care, and research

Maas¹,

Menon²,

Adelson³

et al. 2017

The Lancet Neurology

1,769

1,261

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A concerted effort to tackle the global health problem posed by traumatic brain injury (TBI) is long overdue. TBI is a public health challenge of vast, but insufficiently recognised, proportions. Worldwide, more than 50 million people have a TBI each year, and it is estimated that about half the world's population will have one or more TBIs over their lifetime. TBI is the leading cause of mortality in young adults and a major cause of death and disability across all ages in all countries, with a disproportionate burden of disability and death occurring in low-income and middle-income countries (LMICs). It has been estimated that TBI costs the global economy approximately $US400 billion annually. Deficiencies in prevention, care, and research urgently need to be addressed to reduce the huge burden and societal costs of TBI. This Commission highlights priorities and provides expert recommendations for all stakeholders—policy makers, funders, health-care professionals, researchers, and patient representatives—on clinical and research strategies to reduce this growing public health problem and improve the lives of people with TBI.Additional co-authors: Endre Czeiter, Marek Czosnyka, Ramon Diaz-Arrastia, Jens P Dreier, Ann-Christine Duhaime, Ari Ercole, Thomas A van Essen, Valery L Feigin, Guoyi Gao, Joseph Giacino, Laura E Gonzalez-Lara, Russell L Gruen, Deepak Gupta, Jed A Hartings, Sean Hill, Ji-yao Jiang, Naomi Ketharanathan, Erwin J O Kompanje, Linda Lanyon, Steven Laureys, Fiona Lecky, Harvey Levin, Hester F Lingsma, Marc Maegele, Marek Majdan, Geoffrey Manley, Jill Marsteller, Luciana Mascia, Charles McFadyen, Stefania Mondello, Virginia Newcombe, Aarno Palotie, Paul M Parizel, Wilco Peul, James Piercy, Suzanne Polinder, Louis Puybasset, Todd E Rasmussen, Rolf Rossaint, Peter Smielewski, Jeannette Söderberg, Simon J Stanworth, Murray B Stein, Nicole von Steinbüchel, William Stewart, Ewout W Steyerberg, Nino Stocchetti, Anneliese Synnot, Braden Te Ao, Olli Tenovuo, Alice Theadom, Dick Tibboel, Walter Videtta, Kevin K W Wang, W Huw Williams, Kristine Yaffe for the InTBIR Participants and Investigator

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Section: Brain Banks and Lessons From Neuropathologymentioning

confidence: 99%

Traumatic brain injury: integrated approaches to improve prevention, clinical care, and research

Maas¹,

Menon²,

Adelson³

et al. 2017

The Lancet Neurology

1,769

1,261

View full text Add to dashboard Cite

show abstract

“…Postmortem magnetic resonance imaging (MRI) can help to overcome the obstacles encountered in the detection of structural changes in the brain with in vivo MRI, because it allows the use of higher field strengths and longer acquisition times with a better image resolution and enhanced signal-to-noise ratio [19,36,41]. Although the detection of cortical cerebral microinfarcts could be improved using postmortem 7 T MRI, artefacts still caused false positive results and some histologically verified microinfarcts remained undetectable in MRI scans even upon re-evaluation of the images [46,49].…”

Section: Introductionmentioning

confidence: 99%

Histological correlates of postmortem ultra-high-resolution single-section MRI in cortical cerebral microinfarcts

Yilmazer-Hanke

Mayer

Müller³

et al. 2020

acta neuropathol commun

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The identification of cerebral microinfarctions with magnetic resonance imaging (MRI) and histological methods remains challenging in aging and dementia. Here, we matched pathological changes in the microvasculature of cortical cerebral microinfarcts to MRI signals using single 100 μm-thick histological sections scanned with ultra-highresolution 11.7 T MRI. Histologically, microinfarcts were located in superficial or deep cortical layers or transcortically, compatible with the pattern of layer-specific arteriolar blood supply of the cerebral cortex. Contrary to acute microinfarcts, at chronic stages the core region of microinfarcts showed pallor with extracellular accumulation of lipofuscin and depletion of neurons, a dense meshwork of collagen 4-positive microvessels with numerous string vessels, CD68-positive macrophages and glial fibrillary acidic protein (GFAP)-positive astrocytes. In MRI scans, cortical microinfarcts at chronic stages, called chronic cortical microinfarcts here, gave hypointense signals in T1-weighted and hyperintense signals in T2-weighted images when thinning of the tissue and cavitation and/or prominent iron accumulation were present. Iron accumulation in chronic microinfarcts, histologically verified with Prussian blue staining, also produced strong hypointense T2*-weighted signals. In summary, the microinfarct core was occupied by a dense microvascular meshwork with string vessels, which was invaded by macrophages and astroglia and contained various degrees of iron accumulation. While postmortem ultra-high-resolution single-section imaging improved MRI-histological matching and the structural characterization of chronic cortical cerebral microinfarcts, miniscule microinfarcts without thinning or iron accumulation could not be detected with certainty in the MRI scans. Moreover, string vessels at the infarct margin indicate disturbances in the microcirculation in and around microinfarcts, which might be exploitable in the diagnostics of cortical cerebral microinfarcts with MRI in vivo.

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“…Once a brain has been cut and stained, it cannot be cut and stained again in other ways. Brain samples that are immersed in formalin for a long time suffer from acidity, dehydration (Shepherd et al 2009;Shatil et al 2016), and degradation of the proteins (D'Arceuil and de Crespigny 2007). Therefore, formalin fixation of specimens is not an ideal way to store specimens permanently for use in obtaining neuroanatomical information about the primate brain.…”

Section: Traditional Approaches and Their Limitations For Primate Commentioning

confidence: 99%

“…Its non-destructive properties make MRI an ideal method for studying postmortem (ex vivo) primate brains. Compared to in vivo scanning, ex vivo imaging allows for extremely long MRI experiments that are free of subject motion and other sources of physiological noise (Shatil et al 2016). Therefore, ex vivo MRI produces higher spatial resolution and signal-to-noise ratios than are achievable by in vivo MRI.…”

Section: Advances In Ex Vivo Mri and Their Limitations For Primate Comentioning

confidence: 99%

The Japan Monkey Centre Primates Brain Imaging Repository for comparative neuroscience: an archive of digital records including records for endangered species

et al. 2018

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Advances in magnetic resonance imaging (MRI) and computational analysis technology have enabled comparisons among various primate brains in a three-dimensional electronic format. Results from comparative studies provide information about common features across primates and species-specific features of neuroanatomy. Investigation of various species of nonhuman primates is important for understanding such features, but the majority of comparative MRI studies have been based on experimental primates, such as common marmoset, macaques, and chimpanzee. A major obstacle has been the lack of a database that includes non-experimental primates' brain MRIs. To facilitate scientific discoveries in the field of comparative neuroanatomy and brain evolution, we launched a collaborative project to develop an open-resource repository of non-human primate brain images obtained using ex vivo MRI. As an initial open resource, here we release a collection of structural MRI and diffusion tensor images obtained from 12 species: pygmy marmoset, owl monkey, white-fronted capuchin, crab-eating macaque, Japanese macaque, bonnet macaque, toque macaque, Sykes' monkey, red-tailed monkey, Schmidt's guenon, de Brazza's guenon, and lar gibbon. Sixteen postmortem brain samples from the 12 species, stored in the Japan Monkey Centre (JMC), were scanned using a 9.4-T MRI scanner and made available through the JMC collaborative research program (http:// www.j-monke y.jp/BIR/index _e.html). The expected significant contributions of the JMC Primates Brain Imaging Repository include (1) resources for comparative neuroscience research, (2) preservation of various primate brains, including those of endangered species, in a permanent digital form, (3) resources with higher resolution for identifying neuroanatomical features, compared to previous MRI atlases, (4) resources for optimizing methods of scanning large fixed brains, and (5) references for veterinary neuroradiology. User-initiated research projects beyond these contributions are also anticipated.

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A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts

Cited by 40 publications

References 38 publications

Traumatic brain injury: integrated approaches to improve prevention, clinical care, and research

Traumatic brain injury: integrated approaches to improve prevention, clinical care, and research

Histological correlates of postmortem ultra-high-resolution single-section MRI in cortical cerebral microinfarcts

The Japan Monkey Centre Primates Brain Imaging Repository for comparative neuroscience: an archive of digital records including records for endangered species

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