A Method for Visualization of Kidney Blood Vessels Applied to Studies of the Crush Syndrome.

Schlegel, J.U.; Moses, Jay B.

doi:10.3181/00379727-74-18061

Cited by 28 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9 A fluorometric method was chosen in order to compare perfusion directly with NADH fluorescence in the same volume of cortex. Since both NADH and Thioflavin S absorb light at 366 nm and fluoresce at 450 nm, the cortex was illuminated with ultraviolet light (366 nm) and the 450 nm fluorescence was recorded continuously with a television camera fitted with an image-intensifier.…”

Section: Methodsmentioning

confidence: 99%

Factors limiting regeneration of ATP following temporary ischemia in cat brain.

Welsh¹,

O’Connor²,

Marcy³

et al. 1982

Stroke

View full text Add to dashboard Cite

SUMMARY Cerebral ischemia was induced in cats using bilateral carotid artery occlusion coupled with hemorrhagic hypotension. Thirty minutes of ischemia, which depleted levels of ATP and phosphocreatine throughout the cerebral cortex, was followed by 2-4 hours of recirculation. During the recovery period, cortical perfusion and NADH fluorescence were monitored through a cranial window. Postischemic perfusion, as indicated by transit time, was initially higher than control, but declined to subnormal levels by 60 minutes. NADH fluorescence transients, induced by brief anoxia, also decreased steadily during recirculation, indicating a failure of oxidation-reduction capability. The disappearance of anoxic-NADH transients usually preceded the decline of flow, suggesting that 0 2 delivery was not the factor limiting redox reactions. Furthermore, tissue levels of NADH, which were nearly normal after 2-4 hours of recirculation, did not indicate tissue hypoxia. In spite of normalization of NADH, resynthesis of high energy phosphates was severely impaired. The degree of ATP recovery varied widely in different cortical regions; however, there were two general groups of ATP values -one at 5% and the other at 70% of control levels. In the energy-depleted areas, NADH levels were normal, but the total pool of NAD (NADH + NAD + ) and the tissue content of K + were 43% lower than control. In contrast, the NAD pool and K + content were only slightly diminished in the regions with greater ATP restitution. The results suggest that postischemic resynthesis of ATP may be limited not by inadequate delivery of 0 2 , but rather by defective production of NADH.

show abstract

Section: Methodsmentioning

confidence: 99%

Factors limiting regeneration of ATP following temporary ischemia in cat brain.

Welsh¹,

O’Connor²,

Marcy³

et al. 1982

Stroke

View full text Add to dashboard Cite

show abstract

“…Schlegel and Moses (21) have shown that the fluorescent dye, thioflavin S, when injected intravenously in small amount, diffuses into the wall of the arteries and arterioles and glomeruli and is there retained for a sufficient time so that if the kidney is promptly removed and viewed at low magnification under ultra-violet light all the glomeruli and vessels which had been receiving blood containing the dye stand out in brilliant secondary fluorescence against the darker background of the renal tissue. We shall use this technique at a later point in our investigation and will at that time describe more fully the technical details.…”

Section: Fig 4 Di-ethylene Glycol Poisoning-biopsy From the Cortex mentioning

confidence: 99%

“…In the medulla a brighter zone of primary tissuefluorescence is present in which lie more brilliant efferent capillaries from the cortex. For a more detailed description of this functional-anatomical presentation of the renal circulation the reader is referred to the studies of Schlegel and Moses (21).5…”

Section: Fig 4 Di-ethylene Glycol Poisoning-biopsy From the Cortex mentioning

confidence: 99%

“…Its solution requires experimental examination and will therefore be delayed for later consideration. At Our material consisted of 21 human kidneys from instances of definite clinical poisoning. These were supplemented by the kidneys of 29 experimental animals, dogs and rabbits, which were used in our study of the relation of renal ischemia to toxic renal injury which will be described later.…”

Section: Nephrotoxic Tubular Damagementioning

confidence: 99%

See 1 more Smart Citation

The Pathogenesis of Acute Renal Failure Associated With Traumatic and Toxic Injury. Renal Ischemia, Nephrotoxic Damage and the Ischemuric Episode 1

Oliver¹,

MacDowell²,

Tracy³

1951

J. Clin. Invest.

578

126

View full text Add to dashboard Cite

“…The dye thioflavine S was used in this study because it has the advantage of rapidly penetrating the capillary wall and maintaining the stain in its original position even if the vessels would collapse (4,28,29). Rapid staining by thioflavine S was indicated by the fact that even in experiments with shortest times of dye exposure labeling by this dye was already very intense.…”

Section: Series 2: Estimation Of Functional Capillary Density In the mentioning

confidence: 99%

Effects of isoprenaline on functional capillary density in the subendocardial and subepicardial layer of the rat myocardium

Vetterlein

Schmidt

1980

Basic Res Cardiol

View full text Add to dashboard Cite

The functional capillary density in subepicardial and subendocardial layers of rat heart was measured during rest and during isoprenaline-induced (5.0 microgram X kg-1 X min-1, i.v. over 3 minutes) cardiac stimulation. For determination of the number of perfused capillaries, a fluorescent dye (thioflavine S) was infused into the left atrium; 1, 3, 5 and 10 sec, respectively, after starting dye application, hearts were excised and rapidly cooled down to -50 degrees C. In histological sections capillaries which had been perfused during the dye infusion could be identified and counted. An increase in the number of stained vessels was found in both layers of the myocardium when the time of dye exposure was prolonged. Under these conditions the rise was much smaller in isoprenaline-treated animals, this effect being most marked in the subendocardial layer (3560 +/- 199 cap./mm2, control group; 2190 +/- 30 cap./mm2, isoprenaline-treated group; dye exposure 10 sec). Isoprenaline - at the dose used - induced an increase in total blood flow (3.7 +/- 0.6 ml X min-1 X g-1, control group; 6.8 +/- 0.7 ml X min-1 X g-1, isoprenaline-treated group), however, with a relatively less pronounced increase in the subendocardial blood flow (subendocardial/subepicardial flows: 1.08 +/- 0.13, control group; 0.66 +/- 0.01, isoprenaline-treated group). These results favour the view that isoprenaline-induced relative reduction in the subendocardial blood flow is due to disturbance of perfusion pressure and extravascular compression rather than to exhaustion of the myocardial capillary reserve.

show abstract

A Method for Visualization of Kidney Blood Vessels Applied to Studies of the Crush Syndrome.

Cited by 28 publications

References 0 publications

Factors limiting regeneration of ATP following temporary ischemia in cat brain.

Factors limiting regeneration of ATP following temporary ischemia in cat brain.

The Pathogenesis of Acute Renal Failure Associated With Traumatic and Toxic Injury. Renal Ischemia, Nephrotoxic Damage and the Ischemuric Episode 1

Effects of isoprenaline on functional capillary density in the subendocardial and subepicardial layer of the rat myocardium

Contact Info

Product

Resources

About