A method for the quantification of the pressure dependent 3D collagen configuration in the arterial adventitia

Schrauwen, Jelle T. C.; Vilanova, Anna; Rezakhaniha, Rana; Stergiopulos, Nikolaos; Vosse, F.N. van de; Bovendeerd, P.H.M.

doi:10.1016/j.jsb.2012.06.007

Cited by 55 publications

(63 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The multiphoton imaging modality was well documented in previous studies [51,39,49], should additional information be needed. The imaging resolution of the microscope was set to 0.5 µm in all directions [23,44] Fig. 1 Experimental tension-inflation setup showing (a) a sample cannulated on the needles and loaded, comprising of (b) the tensile machine, (c) the syringe pump, (d) imaging modalities (alternatively optical camera and multiphoton microscope); (e) a schematic representation of the tension-inflation setup, showing the tensile machine, the PBS bath in blue transparency, needle stiffening casings in black transparency, inlet (pink) and outlet (green) needles, the multiphoton microscopes objective in grey (center); and (f) a section sketch of the cannulated arterial sample for tension-inflation testing, presenting the sealing method.…”

Section: Multiphoton Microscopymentioning

confidence: 99%

“…3 (a1) and (a2)), revealing optimal in-plan morphology. In fact, previous studies have investigated the transmural angle (radial direction) of the fibers and showed that it is negligible in comparison to the in-plane angle [42,41,44]. As a result, although the negligible transmurality information is lost, the fibers are represented with maximal effective length, strongly improving the interpretation of their in-plane orientation.…”

Section: Image Analysis and Characterization Of Fiber Kinematicsmentioning

confidence: 99%

“…In particular, the non-linear mechanical behavior of the arterial tissue is often associated with the progressive rearrangements of these different fiber networks, at the 10 to 100 µm scale. In the present contribution, we chose to focus on the adventitial collagen, since it was recently shown to be the fiber network the most prone to mechanically-induced rearrangements [44,12,49,31]. In particular, this network, composed of thick bundles of type I collagen fibers [15], is the major structural component of the adventitia, due to its important load-bearing function preventing over-distensions [26].…”

Section: Introductionmentioning

confidence: 99%

“…Upon mechanical loading, the collagen bundles progressively unfold [8,21]. The measurement of fiber waviness [11,44,53,49] revealed in particular that recruitment, or engagement of collagen fibers, is gradual (unsynchronized among fibers) and starts at a finite strain [23,13]. The process can differ whether the observed vascular region is close or remote from the heart (distal regions vs. proximal regions) [55].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Kinematics of collagen fibers in carotid arteries under tension-inflation loading

Krasny

Magoariec

Morin

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

View full text Add to dashboard Cite

Biomechanics of the extracellular matrix in arteries determines their macroscopic mechanical behavior. In particular, the distribution of collagen fibers and bundles plays a significant role. Experimental data showed that in most arterial walls there are preferred fiber directions. However, the realignment of collagen fibers during tissue deformation is still controversial: whilst authors claim that fibers should undergo affine deformations, others showed the contrary. In order to have an insight about this important question of affine deformations at the microscopic scale, we measured the realignment of collagen fibers in the adventitia layer of carotid arteries using multiphoton microscopy combined with an unprecedented Fourier based method. We compared the realignment for two types of macroscopic loading applied on arterial segments: axial tension under constant pressure (scenario 1) and inflation under constant axial length (scenario 2). Results showed that, although the tissue underwent macroscopic stretches beyond 1.5 in the circumferential direction, fiber directions remained unchanged during scenario 2 loading. Conversely, fibers strongly realigned along the axis direction for scenario 1 loading. In both cases, the motion of collagen fibers did not satisfy affine deformations, with a significant difference between both cases: affine predictions strongly under-estimated fiber reorientations in uniaxial tension and over-estimated fiber reorientations during inflation at constant length. Finally, we explained this specific kinematics of collagen fibers by the complex tension-compression interactions between very stiff collagen fibers and compliant surrounding proteins. A tensegrity representation of the extracellular matrix in the adventitia taking into account these interactions was proposed to model the motion of collagen fibers during tissue deformation.

show abstract

Section: Multiphoton Microscopymentioning

confidence: 99%

Section: Image Analysis and Characterization Of Fiber Kinematicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Kinematics of collagen fibers in carotid arteries under tension-inflation loading

Krasny

Magoariec

Morin

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

View full text Add to dashboard Cite

show abstract

“…However, over 50 years since the work of Wolinsky and Glagov on luminal pressure, arterial morphology is still routinely characterised from 2D cross-sections of unpressurised vessels. A handful of studies have attempted to characterise the internal 3D structure of the pressurised vessel wall, but the methods used, such as confocal microscopy, second-harmonic generation imaging and serial blockface electron microscopy, are all limited to the imaging of small tissue volumes and/or limited tissue components (such as fibrillar collagen) (O'Connell et al, 2008;Schrauwen et al, 2012;Schriefl et al, 2013). In our recent study, we therefore aimed to optimise and develop methods for specimen preparation, microCT imaging and image processing that would allow us to visualise, segment and measure the effects of physiological luminal pressure on the major structures of the arterial wall (Walton et al, 2015).…”

Section: Optical and Electron Microscopy In Three Dimensionsmentioning

confidence: 99%

Three-dimensional visualisation of soft biological structures by X-ray computed micro-tomography

Shearer

Bradley

Hidalgo‐Bastida

et al. 2016

Journal of Cell Science

View full text Add to dashboard Cite

Whereas the two-dimensional (2D) visualisation of biological samples is routine, three-dimensional (3D) imaging remains a time-consuming and relatively specialised pursuit. Current commonly adopted techniques for characterising the 3D structure of non-calcified tissues and biomaterials include optical and electron microscopy of serial sections and sectioned block faces, and the visualisation of intact samples by confocal microscopy or electron tomography. As an alternative to these approaches, X-ray computed micro-tomography (microCT) can both rapidly image the internal 3D structure of macroscopic volumes at sub-micron resolutions and visualise dynamic changes in living tissues at a microsecond scale. In this Commentary, we discuss the history and current capabilities of microCT. To that end, we present four case studies to illustrate the ability of microCT to visualise and quantify: (1) pressure-induced changes in the internal structure of unstained rat arteries, (2) the differential morphology of stained collagen fascicles in tendon and ligament, (3) the development of Vanessa cardui chrysalises, and (4) the distribution of cells within a tissue-engineering construct. Future developments in detector design and the use of synchrotron X-ray sources might enable real-time 3D imaging of dynamically remodelling biological samples.

show abstract

A quarter of a century biomechanical rupture risk assessment of abdominal aortic aneurysms. Achievements, clinical relevance, and ongoing developments

Gasser

Miller

Polzer

et al. 2022

Numer Methods Biomed Eng

View full text Add to dashboard Cite

Abdominal aortic aneurysm (AAA) disease, the local enlargement of the infrarenal aorta, is a serious condition that causes many deaths, especially in men exceeding 65 years of age. Over the past quarter of a century, computational biomechanical models have been developed towards the assessment of AAA risk of rupture, technology that is now on the verge of being integrated within the clinical decision-making process. The modeling of AAA requires a holistic understanding of the clinical problem, in order to set appropriate modeling assumptions and to draw sound conclusions from the simulation results. In this article we summarize and critically discuss the proposed modeling approaches and report the outcome of clinical validation studies for a number of biomechanics-based rupture risk indices. Whilst most of the aspects concerning computational mechanics have already been settled, it is the exploration of the failure properties of the AAA wall and the acquisition of robust input data for simulations that has the greatest potential for the further improvement of this technology. K E Y W O R D Sabdominal aortic aneurysm, modeling, ruptuer risk assessment, vascular biomechanics | WHAT IS AAAWhilst an aneurysm, a permanent local enlargement of an artery by more than 50% of its diameter, may affect all arteries, the infrarenal aorta is most vulnerable to this disease and often leads to the development of an abdominal aortic aneurysm (AAA). The formation of AAAs is promoted by factors such as old age, male gender, smoking, and high mean arterial pressure (MAP). Furthermore, it is over-represented in persons with a family history of AAA and patients with coronary heart disease and Chronic Obstructive Pulmonary Disease. Aortic aneurysms can also present in patients with rare genetic diseases, such as Ehlers-Danlos syndrome type IV, Marfan syndrome, Loeys-Dietz syndrome, and fibromuscular dysplasia. Most AAAs remain small and therefore do not necessitate clinical intervention. However, an aneurysm may grow to a size that could result in aortic rupture, a life-threatening event. In urban areas only 50% of

show abstract

A method for the quantification of the pressure dependent 3D collagen configuration in the arterial adventitia

Cited by 55 publications

References 30 publications

Kinematics of collagen fibers in carotid arteries under tension-inflation loading

Kinematics of collagen fibers in carotid arteries under tension-inflation loading

Three-dimensional visualisation of soft biological structures by X-ray computed micro-tomography

A quarter of a century biomechanical rupture risk assessment of abdominal aortic aneurysms. Achievements, clinical relevance, and ongoing developments

Contact Info

Product

Resources

About