A method for the preferential isolation of actinomycetes from soils

Agate, A. D.; Bhat, Jayashree S.

doi:10.1007/bf02046072

Cited by 17 publications

(10 citation statements)

References 4 publications

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“…So, the suitability of some media for enumeration and isolation of desert actinomycetes was investigated by inoculating a series of dilutions of soils onto three different media to select the most appropriate medium for the development of actinomycetes. The media used were glucose-yeast extract (GYE), which was recommended by many investigators to be the most suitable medium that gives a high ratio of actinomycetes and also has the advantage that it can be easily prepared (Agate and Bhat 1963), soil extract and a new minimal medium (MM). The later two media were selected as low-nutrient media which resemble the poor environments in desert soils that may reveal novel actinomycete strains.…”

Section: Resultsmentioning

confidence: 99%

A new preferential medium for enumeration and isolation of desert actinomycetes

Hozzein

Ali

Rabie

2007

World J Microbiol Biotechnol

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In order to facilitate the discovery of novel actinomycetes from the Egyptian deserts, which can be useful as new sources for bioactive metabolites, different media for enumeration and isolation of desert actinomycetes have been tested. For this purpose, 30 soil samples from different six sites representing the Western and Eastern deserts of Egypt were collected. The two deserts are considered hyper-arid and the soil characteristics were determined. The media used were glucose-yeast extract agar, soil extract agar and a new minimal medium (MM) containing glucose, yeast extract and mineral salts. The effects of the soil characteristics on the total viable actinomycete counts on the three media were evaluated. The results showed that the highest actinomycete count in samples from five out of six sites was obtained on MM. Also MM was more selective for actinomycetes and significantly decreased the number of fungal colonies and to a lower extent the number of bacterial colonies. Moreover, it supported the development of different and diverse groups of actinomycetes. From the results obtained in this study, MM is a new useful medium for enumeration and selective isolation of actinomycetes from the desert soils.

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Section: Resultsmentioning

confidence: 99%

A new preferential medium for enumeration and isolation of desert actinomycetes

Hozzein

Ali

Rabie

2007

World J Microbiol Biotechnol

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“…A serial dilution was performed by adding 1 g of soil to 99 ml of sterile distilled water and diluted serially to 10 −6 . Bacterial population was determined by method adopted by Paul and Clark (1998), fungi by the method described by Harrigan and McCance (1990) and actinomycetes by the procedure of Agates and Bhat (1963).…”

Section: Soil Microbial Analysismentioning

confidence: 99%

Post tsunami changes in soil properties of Andaman Islands, India

Nayak

Damodaran

Singh

et al. 2009

Environ Monit Assess

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A post tsunami study was conducted to assess the changes in soil properties in the Andaman Island, in India. The present study reported tsunami led conversion of acid soils to saline acid soils and acid sodic soils to acid saline sodic soils in the areas South Andaman inundated during tsunami and permanently receded later and in the low-lying area submerged during high tides. Upon intense leaching acid saline soils and acid saline sodic may further develop typical characteristics of acidic soils and acidic sodic soil, respectively. The soil at Guptapara inundated almost due to tsunami with minimal pyrite oxidation has potential to develop into highly acidic soils upon drainage. The tsunami by and large has modified some depositional layer affecting the salt accumulation to a greater extent and iron to a lesser extent and least to sodicity.

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“…Tsao, Leben & Keitt (1960) reported increased selective development of actinomycetes when air-dried soil was re-moistened, mixed with calcium carbonate and incubated at 28". Agate & Bhat (1963) attempted suppression of bacteria and fungi by pre-incubation of soil at 110' for 10 min. and the use of dried plates.…”

Section: Introductionmentioning

confidence: 99%

Use of Antibiotics for Selective Isolation and Enumeration of Actinomycetes in Soil

Williams

Davies

1965

Journal of General Microbiology

191

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SuMlMARYSeveral antibiotics were tested against a range of actinomycetes, bacteria and fungi representing types found in soil. From these tests four antibiotics, nystatin (50 pg./ml.), actidione (50 pg./ml.), polymyxin B sulphate (5.0 pg./ml.) and sodium penicillin (1.0 ,ug./ml.), were selected for incorporation into a starch +casein medium to achieve selective growth of actinomycetes on soil dilution plates. This mixture of antibiotics was tested with a number of soils and its efficiency compared with several other methods for selective development of actinomycete colonies. The most suitable mixture for the enumeration of soil actinomycete colonies was starch + casein medium with the two antifungal antibiotics (nystatin, actidione) ; for isolation of actinomycete colonies the same medium with all four antibiotics was the most satisfactory.

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A method for the preferential isolation of actinomycetes from soils

Cited by 17 publications

References 4 publications

A new preferential medium for enumeration and isolation of desert actinomycetes

A new preferential medium for enumeration and isolation of desert actinomycetes

Post tsunami changes in soil properties of Andaman Islands, India

Use of Antibiotics for Selective Isolation and Enumeration of Actinomycetes in Soil

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