A method for the measurement of in line pistachio aflatoxin concentration based on the laser induced fluorescence spectroscopy

Paghaleh, Soodeh Jamali; Argani, Hassan; Marashi, Seyed Mohammad Bagher; Rahimi, Mojtaba; Bahrampour, Abbas

doi:10.1016/j.jlumin.2014.12.057

Cited by 20 publications

(7 citation statements)

References 19 publications

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“…Spectroscopy techniques have been applied for rapid and real-time analysis for mycotoxins, with little or no sample preparation, without destroying the sample [114]. Paghaleh et al [115] developed a method based on the laser induced fluorescence spectroscopy, using a UV laser (λ = 308 nm) for in line measurement of the concentration of AFs in pistachio nuts, without sample preparation, and results are in agreement with the HPLC method. Wu and Xu [116] developed a multiplexing fiber optic laser induced fluorescence spectroscopy for detection of AFB1 in pistachios, using five wavelengths between 440 and 564 nm because physical and chemical characteristics of pistachios at different positions of contaminated products are unequal or nonuniform.…”

Section: Biosensorsmentioning

confidence: 93%

Mycotoxins in Pistachios (Pistacia vera L.): Methods for Determination, Occurrence, Decontamination

Mateus

Barros

Pena

et al. 2021

Toxins

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The consumption of pistachios (Pistacia vera L.) has been increasing, given their important benefit to human health. In addition to being an excellent nutritional source, they have been associated with chemical hazards, such as mycotoxins, resulting in fungal contamination and its secondary metabolism. Aflatoxins (AFs) are the most common mycotoxins in pistachio and the most toxic to humans, with hepatotoxic effects. More mycotoxins such as ochratoxin A (OTA), fumonisins (FBs), zearalenone (ZEA) and trichothecenes (T2, HT2 and DON) and emerging mycotoxins have been involved in nuts. Because of the low levels of concentration and the complexity of the matrix, the determination techniques must be very sensitive. The present paper carries out an extensive review of the state of the art of the determination of mycotoxins in pistachios, concerning the trends in analytical methodologies for their determination and the levels detected as a result of its contamination. Screening methods based on immunoassays are useful due to their simplicity and rapid response. Liquid chromatography (LC) is the gold standard with new improvements to enhance accuracy, precision and sensitivity and a lower detection limit. The reduction of Aspergillus’ and aflatoxins’ contamination is important to minimize the public health risks. While prevention, mostly in pre-harvest, is the most effective and preferable measure to avoid mycotoxin contamination, there is an increased number of decontamination processes which will also be addressed in this review.

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Section: Biosensorsmentioning

confidence: 93%

Mycotoxins in Pistachios (Pistacia vera L.): Methods for Determination, Occurrence, Decontamination

Mateus

Barros

Pena

et al. 2021

Toxins

View full text Add to dashboard Cite

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“…LIF spectroscopy has shown interesting results in the detection of AFB 1 in cereal grains and nuts because of its intense light source and narrow‐medium bandwidth. While most studies detected samples at the single‐kernel level (Paghaleh et al., 2015; Smeesters et al., 2015, 2016; Wu & Xu, 2019, 2020), Wang et al. (2022) attempted to detect rice samples at the bulk level.…”

Section: Analysis Related To Lif and Led‐if Techniques At Laboratory ...mentioning

confidence: 99%

“…While most studies detected samples at the single-kernel level (Paghaleh et al, 2015;Smeesters et al, 2015Smeesters et al, , 2016Wu & Xu, 2019, 2020, Wang et al (2022) attempted to detect rice samples at the bulk level. Intact rice samples were placed in quartz cuvettes for fluorescence acquisition.…”

Section: 25mentioning

confidence: 99%

Toward commercial applications of LED and laser‐induced fluorescence techniques for food identity, quality, and safety monitoring: A review

Peng

et al. 2023

Comp Rev Food Sci Food Safe

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The assessment of food safety and quality is a matter of paramount importance, especially considering the challenges posed by climate change. Convenient, eco‐friendly, and non‐destructive techniques have attracted extensive attention in the food industry because they can retain food safety and quality. Fluorescence radiation, the process by which fluorophore emits light upon the absorption of ultraviolet or visible light, offers the advantages of high sensitivity and selectivity. The use of excitation–emission matrix (EEM) has been extensively explored in the food industry, but on‐site detection of EEMs remain a challenge. To address this limitation, laser‐induced fluorescence (LIF) and light emitting diode‐induced fluorescence (LED‐IF) have been implemented in many cases to facilitate the transition of fluorescence measurements from the laboratory to commercial applications. This review provides an overview of the application of commercially available LIF/LED‐IF devices for non‐destructive food measurement and recent studies that focus on the development of LIF/LED‐IF devices for commercial applications. These studies were categorized into two stages: the preliminary exploration stage, which emphasizes the selection of an appropriate excitation wavelength based on the combination of EEM and chemometrics, and the pre‐application stage, where experiments were conducted on scouting with specific excitation wavelength. Although commercially available devices have emerged in many research fields, only a limited number have been reported for use in the food industry. Future studies should focus on enhancing the diversity of test samples and parameters that can be measured by a single device, exploring the application of LIF techniques for detecting low‐concentration substances in food, investigating more quantitative approaches, and developing embedded computing devices.

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“…Fluorescence emission from samples can also be excited with laser light. Paghaleh et al (2015) [ 24 ] used laser (308 nm)-induced UV fluorescence spectroscopy for aflatoxin detection in fungus-infected pistachio nuts.…”

Section: Introductionmentioning

confidence: 99%

A Low-Cost, Portable Device for Detecting and Sorting Aflatoxin-Contaminated Maize Kernels

Yao

Zhu

Kincaid

et al. 2023

Toxins

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Aflatoxin contamination of maize is a major food safety issue worldwide. The problem is of special significance in African countries because maize is a staple food. This manuscript describes a low-cost, portable, non-invasive device for detecting and sorting aflatoxin-contaminated maize kernels. We developed a prototype employing a modified, normalized difference fluorescence index (NDFI) detection method to identify potentially aflatoxin-contaminated maize kernels. Once identified, these contaminated kernels can be manually removed by the user. The device consists of a fluorescence excitation light source, a tablet for image acquisition, and detection/visualization software. Two experiments using maize kernels artificially infected with toxigenic Aspergillus flavus were implemented to evaluate the performance and efficiency of the device. The first experiment utilized highly contaminated kernels (71.18 ppb), while mildly contaminated kernels (1.22 ppb) were used for the second experiment. Evidently, the combined approach of detection and sorting was effective in reducing aflatoxin levels in maize kernels. With a maize rejection rate of 1.02% and 1.34% in the two experiments, aflatoxin reduction was achieved at 99.3% and 40.7%, respectively. This study demonstrated the potential of using this low-cost and non-invasive fluorescence detection technology, followed by manual sorting, to significantly reduce aflatoxin levels in maize samples. This technology would be beneficial to village farmers and consumers in developing countries by enabling safer foods that are free of potentially lethal levels of aflatoxins.

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A method for the measurement of in line pistachio aflatoxin concentration based on the laser induced fluorescence spectroscopy

Cited by 20 publications

References 19 publications

Mycotoxins in Pistachios (Pistacia vera L.): Methods for Determination, Occurrence, Decontamination

Mycotoxins in Pistachios (Pistacia vera L.): Methods for Determination, Occurrence, Decontamination

Toward commercial applications of LED and laser‐induced fluorescence techniques for food identity, quality, and safety monitoring: A review

A Low-Cost, Portable Device for Detecting and Sorting Aflatoxin-Contaminated Maize Kernels

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