A method for the isolation of the caribbean palytoxin (C-PTX) from the coelenterate (zooanthid) Palythoa caribaeorum

Béress, L.; Zwick, Joachim; Kolkenbrock, Hansjörg; Kaul, Pushkar N.; Wassermann, O.

doi:10.1016/0041-0101(83)90013-2

Cited by 55 publications

(23 citation statements)

References 20 publications

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“…The hypothesis of a symbiotic bacterial origin for this group of toxins has been explored in some recent works [177,178]. Although the palytoxin-containing zoanthid organisms are located in tropical waters, the dinoflagellates of the genus Ostreopsis have a worldwide distribution and toxin producing species have been described not only in tropical and subtropical waters, but also in the Mediterranean Sea [179-182]. Humans show symptoms of intoxication by palytoxin following ingestion of contaminated seafood or exposure to seawater aerosol in bathing areas.…”

Section: Palytoxins and Ostreocinsmentioning

confidence: 99%

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

Vilariño

Fonfría

Louzao

et al. 2009

Sensors

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Marine toxins are currently monitored by means of a bioassay that requires the use of many mice, which poses a technical and ethical problem in many countries. With the exception of domoic acid, there is a legal requirement for the presence of other toxins (yessotoxin, saxitoxin and analogs, okadaic acid and analogs, pectenotoxins and azaspiracids) in seafood to be controlled by bioassay, but other toxins, such as palytoxin, cyclic imines, ciguatera and tetrodotoxin are potentially present in European food and there are no legal requirements or technical approaches available to identify their presence. The need for alternative methods to the bioassay is clearly important, and biosensors have become in recent years a feasible alternative to animal sacrifice. This review will discuss the advantages and disadvantages of using biosensors as alternatives to animal assays for marine toxins, with particular focus on surface plasmon resonance (SPR) technology.

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Section: Palytoxins and Ostreocinsmentioning

confidence: 99%

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

Vilariño

Fonfría

Louzao

et al. 2009

Sensors

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“…The hypothesis of a symbiotic bacterial origin for this group of toxins has been explored in some recent works [177,178]. Although the palytoxin-containing zoanthid organisms are located in tropical waters, the dinoflagellates of the genus Ostreopsis have a worldwide distribution and toxin producing species have been described not only in tropical and subtropical waters, but also in the Mediterranean Sea [179][180][181][182]. Humans show symptoms of intoxication by palytoxin following ingestion of contaminated seafood or exposure to seawater aerosol in bathing areas.…”

Section: Palytoxins and Ostreocinsmentioning

confidence: 99%

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

Botana¹,

Vilariño²,

Alfonso³

et al. 2012

Springer Protocols Handbooks

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“…Cells were grown in 25 cm 2 tissue culture flasks at 37°C in a humidified atmosphere containing 5% CO 2 , and were subcultured by transferring cells released by the application of 0.1% trypsin-EDTA [52,53].…”

Section: Cell Line Culturementioning

confidence: 99%

“…Palytoxin is one of the largest and highly potent marine toxins first isolated from zooanthids of the genus Palythoa [1][2][3]. It has been also found in sea anemones, polychaete worms, crabs and herbivorous fishes [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Production of Functionally Active Palytoxin-like Compounds by Mediterranean <i>Ostreopsis cf. siamensis</i>

Cagide

Louzao²,

Espiña³

et al. 2009

Cell Physiol Biochem

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Background and purpose: Dinoflagellates from the genus Ostreopsis have been related to the production of palytoxin and analogues. Based on that, this paper describes functional studies of crude extracts from Ostreopsis cf. siamensis collected in the Mediterranean Sea in order to biochemically characterize their toxic compounds. Methods: We compared the effects of 5 crude dinoflagellates extracts with a commercially available palytoxin and a purified Ostreopsis ovata extract on metabolic activity, membrane potential, and cytosolic calcium levels by using fluorescent dyes. Results: All the extracts resulted to be neurotoxic. In addition, all of them induced a membrane depolarization and a calcium increment that were abolished when preincubating with ouabain, an inhibitor of the Na⁺/K⁺ pump. Conclusion: The effects observed were quite close to those induced by palytoxin and the Ostreopsis ovata extract as well, suggesting that Ostreopsis cf. siamensis is actually producing palytoxin-like compounds that are highly toxic and functionally active.

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A method for the isolation of the caribbean palytoxin (C-PTX) from the coelenterate (zooanthid) Palythoa caribaeorum

Cited by 55 publications

References 20 publications

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

Production of Functionally Active Palytoxin-like Compounds by Mediterranean <i>Ostreopsis cf. siamensis</i>

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