A method for the estimation of serum creatine kinase and its use in comparing creatine kinase and aldolase activity in normal and pathological sera

Hughes, B.R.

doi:10.1016/0009-8981(62)90137-7

Cited by 523 publications

(163 citation statements)

References 9 publications

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“…The creatine formed was estimated by the colorimetric method of Hughes. 21 The color was developed by addition of 100 mL 2% a-naphtol and 100 mL 0.05% diacetyl in a final volume of 1 mL and read spectrophotometrically after 20 min at 540 nm. Results were expressed as units/min x mg protein.…”

Section: Activity Of Creatine Kinasementioning

confidence: 99%

Fluvoxamine alters the activity of energy metabolism enzymes in the brain

Ferreira

Cardoso

Jeremias

et al. 2014

Rev. Bras. Psiquiatr.

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Objective: Several studies support the hypothesis that metabolism impairment is involved in the pathophysiology of depression and that some antidepressants act by modulating brain energy metabolism. Thus, we evaluated the activity of Krebs cycle enzymes, the mitochondrial respiratory chain, and creatine kinase in the brain of rats subjected to prolonged administration of fluvoxamine. Methods: Wistar rats received daily administration of fluvoxamine in saline (10, 30, and 60 mg/kg) for 14 days. Twelve hours after the last administration, rats were killed by decapitation and the prefrontal cortex, cerebral cortex, hippocampus, striatum, and cerebellum were rapidly isolated. Results: The activities of citrate synthase, malate dehydrogenase, and complexes I, II-III, and IV were decreased after prolonged administration of fluvoxamine in rats. However, the activities of complex II, succinate dehydrogenase, and creatine kinase were increased. Conclusions: Alterations in activity of energy metabolism enzymes were observed in most brain areas analyzed. Thus, we suggest that the decrease in citrate synthase, malate dehydrogenase, and complexes I, II-III, and IV can be related to adverse effects of pharmacotherapy, but long-term molecular adaptations cannot be ruled out. In addition, we demonstrated that these changes varied according to brain structure or biochemical analysis and were not dose-dependent.

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Section: Activity Of Creatine Kinasementioning

confidence: 99%

Fluvoxamine alters the activity of energy metabolism enzymes in the brain

Ferreira

Cardoso

Jeremias

et al. 2014

Rev. Bras. Psiquiatr.

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“…The creatine formed was estimated according to the colorimetric method of Hughes (1962). CK activity was assayed in the reaction mixture contained 65 mM Tris-HCl buffer, pH 7.5, 7 mM phosphocreatine, 9 mM MgSO 4 , and 1 µg of protein (cytosolic or mitochondrial-rich fraction), in a final volume of 0.1 ml.…”

Section: Ck Activitymentioning

confidence: 99%

Amyloid-β peptide absence in short term effects on kinase activity of energy metabolism in mice hippocampus and cerebral cortex

Ianiski

Rech

Nishihira

et al. 2016

An. Acad. Bras. Ciênc.

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Considering that Alzheimer's disease is a prevalent neurodegenerative disease worldwide, we investigated the activities of three key kinases: creatine kinase, pyruvate kinase and adenylate kinase in the hippocampus and cerebral cortex in Alzheimer's disease model. Male adult Swiss mice received amyloid-β or saline. One day after, mice were treated with blank nanocapsules (17 ml/kg) or meloxicam-loaded nanocapsules (5 mg/kg) or free meloxicam (5 mg/kg). Treatments were performed on alternating days, until the end of the experimental protocol. In the fourteenth day, kinases activities were performed. Amyloid-β did not change the kinases activity in the hippocampus and cerebral cortex of mice. However, free meloxicam decrease the creatine kinase activity in mitochondrial-rich fraction in the group induced by amyloid-β, but for the cytosolic fraction, it has raised in the activity of pyruvate kinase activity in cerebral cortex. Further, meloxicam-loaded nanocapsules administration reduced adenylate kinase activity in the hippocampus of mice injected by amyloid-β. In conclusion we observed absence in short-term effects in kinases activities of energy metabolism in mice hippocampus and cerebral cortex using amyloid-β peptide model. These findings established the foundation to further study the kinases in phosphoryltransfer network changes observed in the brains of patients post-mortem with Alzheimer's disease.

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“…), abdominal cavities were opened and blood was drawn from the abdominal vena cava. Serum diluted 1:25 was assayed for CK activity, according to the method of Hughes (1962) and King (1965), as per the Auto Span diagnostics kit.…”

Section: Myotoxic Activitymentioning

confidence: 99%