A method for the determination of carnitine in the picomole range

Mitochondrial and peroxisomal fatty acid oxidation capacities increase in the skeletal muscles of young pigs during early postnatal development but are not affected by cold stress

“…Briefly, the sonicated homogenates were mixed with 0.45 N HClO 4 and centrifuged at 1500 × g for 10 min at 4 o C. Acid-soluble carnitine was recovered in the supernatant, while long-chain acylcarnitine stayed in the pellet. Measurement of acid-soluble carnitine was performed with the radiochemical method of Cederblad and Lindstedt [16] using 21 µM [1-14 C]acetyl-CoA (specific radioactivity, 1.02 µCi·µmol -1 ) as the substrate. The reaction was initiated by adding 2 units of carnitine acetyl-transferase.…”

Effects of Short-Term Leptin Exposure on Triglyceride Deposition in Rat Liver

“…For the determination of free and short-chain acylcarnitine a perchloric acid extract of a part of the frozen livers was used. The carnitine esters were saponified and assayed as free carnitine by a radioenzymatic method, 20 with the following modifications: HEPES buffer was used instead of Tris buffer and N-ethylmaleimide was used instead of tetrathionate.…”

Carnitine and/or GABA Supplementation Increases Immune Function and Changes Lipid Profiles and Some Lipid Soluble Vitamins in Mice Chronically Administered Alcohol

“…Non-collagen protein (NCP) was determined using a Bio-Rad protein assay (BIO-RAD Co., USA), based on the method of Bradford (23). Non esterified carnitine (NEC), acid-soluble acylcarnitine (ASAC), and acid-insoluble acylcarnitine (AIAC) in serum and tissues, were determined by the radio-enzymatic procedure of Cederblad and Lindstedt (24), as modified by Sachan et al (25). In this method, AIAC was precipitated with perchloric acid and centrifugation leaving the ASAC and NEC in the supernatant.…”