A method for the determination of aldosterone, cortisol and corticosterone in biological extracts, particularly applied to human urine

Ayres, P. J.; Garrod, Oliver; Simpson, Sylvia A.; Tait, John

doi:10.1042/bj0650639

Cited by 99 publications

(17 citation statements)

References 20 publications

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“…This can be explained at least in part by the greater yield obtained by the longer period of hydrolysis employed in the method presented. The normal range obtained is in close agreement with that obtained by Ayres and coworkers (1). This most likely is coincidental, since they used hydrolysis for only twenty-four hours, but "partially corrected' 7 for overall losses in the method.…”

Section: Normal Valuessupporting

confidence: 86%

“…The normal values obtained in this study are somewhat higher than those reported by other workers who employed methods yielding similar recoveries (1,2,14). This can be explained at least in part by the greater yield obtained by the longer period of hydrolysis employed in the method presented.…”

Section: Normal Valuescontrasting

confidence: 81%

“…Of the published physico-chemical procedures, at least 2 appear to have the necessary specificity and accuracy, namely, the method of Ayres, Garrod, Simpson and Tait (1) and that of Nowaczynski, Koiw and Genest (2). The method of Nowaczynski and coworkers was set up in our laboratories for routine analyses.…”

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confidence: 99%

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Chemical Determination of Aldosterone in Urine

Sobel

Henry

Golub

et al. 1959

The Journal of Clinical Endocrinology & Metabolism

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A method for the determination of aldosterone in urine is presented, which includes the following steps: acid hydrolysis and extraction, column chromatography on silica gel, 2 paper chromatographic systems, acetylation, a third paper chromatographic system, and fluorometry directly on the paper using alkaline fluorescence. Data are presented on precision, recovery, duration of acid hydrolysis, acid versus enzymatic hydrolysis, stability of urine samples and normal values.T HE importance of aldosterone in the regulation of water and salt metabolism has made highly desirable the availability of a specific chemical method of reasonable accuracy for its determination. Of the published physico-chemical procedures, at least 2 appear to have the necessary specificity and accuracy, namely, the method of Ayres, Garrod, Simpson and Tait (1) and that of Nowaczynski, Koiw and Genest (2). The method of Nowaczynski and coworkers was set up in our laboratories for routine analyses. One urine specimen was encountered, however, in which substitution of acetylation and the Bush B3 system for the Bush B5 system employed as the third paper in Nowaczynski's method yielded considerably lower results. This led to a study of the method, and the introduction of some modifications of the technic of Nowaczynski which are believed to increase the yield and simplify the quantitation in the final step. Essentially, these modifications consist of (a) an increase in the time of hydrolysis, (b) acetylation and substitution of the Bush B3 system for the Bush B5 system used by Nowaczynski for the final separation of aldosterone on paper, and (c) fluorometric estimation by carrying out the Bush alkaline fluorescence reaction directly on paper, a procedure used by Ayres, Simpson and Tait (3). A detailed description is given of the technics used, with data on reproducibility, effect of varying the time of hydrolysis, recovery of known standards, normal values, and stability of urine samples.

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Section: Normal Valuessupporting

confidence: 86%

Section: Normal Valuescontrasting

confidence: 81%

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Chemical Determination of Aldosterone in Urine

Sobel

Henry

Golub

et al. 1959

The Journal of Clinical Endocrinology & Metabolism

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“…The eluate was acetylated and re-run in the system methanol : water, 85:15, as the stationary phase, and petroleum ether : toluene, 2:1 as the mobile phase. Aldosterone was then measured on the paper by the soda fluorescence reaction using a specially constructed fluorimeter (Ayres, Garrod, Simpson & Tait, 1957).…”

Section: Methodsmentioning

confidence: 99%

Effect of Sodium Restriction and Experimental Nephrosis on Tissue Spaces and Tissue Sodium Distribution in the Rat

Leonard¹

1962

Journal of Endocrinology

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Aldosterone secretion is increased in rats on a diet low in sodium content or in rats with nephrosis induced by administration of an aminonucleoside. Distributions of water and sodium in heart, liver, adrenals and abdominal muscle were compared in nephrotic rats, sodium-restricted rats and rats maintained on a normal diet. In nephrotic animals total tissue sodium increased significantly in liver, heart and abdominal muscle and was associated with increased intracellular sodium (both absolute amount and concentration) in these tissues as well as in the adrenals; extracellular sodium decreased in heart and adrenals and increased in abdominal muscle. In nephrotic rats increased total tissue water in liver and abdominal muscle was associated with increased extracellular water while increased total water in adrenals was accompanied by increased intracellular water. The results in sodium-restricted rats were similar to those in nephrotic rats except that no changes were found in abdominal muscle and that water content of liver was unchanged.

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“…(ii) 20 The adrenal glands were first described in 1563, when at that time Bartholomaeus Eustachius noted them as 11 Glandulae renibus incumbentes 11 • Since then, they have gone under several names; ncapsulae renales 11 , 11 glandulae renales", 11 capsulae atrabilariae 11 and the more familiar name of suprarenal capsules. In the 17th century the prevalent idea was that the adrenals were functional only in foetal life.…”

Section: Table Of Contentsmentioning

confidence: 99%