A Method for the Determination of Desoxyribonucleic Acid, Ribonucleic Acid, and Phosphoproteins in Animal Tissues

Schmidt, Gerhard; Thannhauser, S. J.

doi:10.1016/s0021-9258(17)41524-9

Cited by 2,417 publications

(53 citation statements)

References 6 publications

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“…DNA and RNA extraction was performed by a modification of the method of Schmidt & Thanhauser (1945). To the cerebellum stored in liquid nitrogen, ten times the volume of icecold 10% trichloroacetic acid (by mass) was added and homogenized in Potter homogenizer of 0.5 ml and centrifuged at 3000 rev min-1 in a Sorval centrifuge.…”

Section: D) Biochemical Studiesmentioning

confidence: 99%

Anatomical, Physiological and biochemical studies of the cerebellum fromreelermutant mouse

Mariani

Crépel

Mikoshiba

et al. 1977

Phil. Trans. R. Soc. Lond. B

381

122

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The cerebellum of the homozygous reeler mouse shows a marked reduction in size and in the number of fissures, its dry mass and DNA content are respectively and j—J of those of normal animals. Its high content in the P400 protein, which is abundant in the Purkinje cell, indicates that the decrease in cell number associated with the fall in DNA affects primarily the granular cells. The anatomy of the reeler cerebellum is rather unique: a thin cortex with almost normal molecular, granular and Purkinje cell layers embracing a central mass of closely packed large neurons, mostly Purkinje cells. Purkinje cells may therefore be found in four different cellular environments: (1) at their normal position in the superficial cortical structure; (2) within the granular layer; (3) intermingled with white matter in the central mass; (4) overlapping with neurons of the deep cerebellar nuclei. The reeler cerebellum therefore offers a model to study to what extent local cellular interactions are required to achieve the planar organization of the Purkinje cell dendrites and the normal synaptic investment of these cells. Concerning the three-dimensional shape of Purkinje cells, only the rare ones located at their normal position and receiving a normal ratio of all their synaptic afferences succeed to develop a characteristic dendritic pattern. Purkinje cells within the granular layer show three distinct patterns of dendritic arrangements. The variation in shape of the Purkinje cell dendrites located in the central agranular mass mimics that described in other agranular cerebella: in particular they show randomly oriented dendrites devoid of spiny branchlets. Concerning the cerebellar circuitry, the specificity of most of the synaptic connections is preserved, despite important disorders in Purkinje cell distribution.Several important differences with the normal cerebellum have, however, been observed at the level of the Purkinje cell: (i) The density of climbing fibre varicosities increases in the central cerebellar mass, where Purkinje cells are deprived of parallel fibre afferences. In addition, electrophysiological studies reveal that, at this position, the response of the Purkinje cells to climbing fibre stimulation is graded by steps as a function of stimulus intensity instead of being all-or-none as found in the superficial cortex or in normal cerebellum. These deep Purkinje cells receive therefore several climbing fibres instead of only one as in normal adult cerebellum, (ii) Ectopic synapses (somato-dendritic and dendro-dendritic) between the soma and/or the dendrite of the granule cell as presynaptic element and mainly the Purkinje cell dendrites as postsynaptic element may form, (iii) Heterologous synapses between mossy fibres and Purkinje cell spines are found in the granular layer and within the central mass. The electrophysiological studies show that these synapses are functional.

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Section: D) Biochemical Studiesmentioning

confidence: 99%

Anatomical, Physiological and biochemical studies of the cerebellum fromreelermutant mouse

Mariani

Crépel

Mikoshiba

et al. 1977

Phil. Trans. R. Soc. Lond. B

381

122

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“…RNA and DNA and DNA were determined by the method of (Schmidt and Thannhauser, 1945). For the extraction, 0.5 gm plant material was crushed in mortar with 0.5 PCA (chilled) and kept for 30 minutes at room temperature and then centrifuged at 1000 rpm for 5 minutes.…”

Section: Extraction and Estimation Of Nucleic Acidmentioning

confidence: 99%

Topsin-M application affected nucleic acid, amino acid and phenol contents of Cicer arietinum L

2015

Int. J. Biosci.

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The effect of elevated concentrations of fungicide Topsin-M on nucleic acid, amino acid and phenols of Cicer arietinum L. was studied. Topsin-M was applied to Cicer arietinum L. foliage at seedling and at fruiting stage. It was observed that nucleic acid (DNA and RNA) contents of the crop decreased, while amino acid and phenolic contents increased as a result of such foliar application of Topsin-M. We found that amino acids such as glycine, asparagines, alanine, methionine, phenyl alanine were present both in control and treated plants, while cystein, proline, tryptophane and valine contents were found in higher quantities in treated samples as compared to control ones. Reduction in nucleic acid, changes in amino acids and an increase in total phenolic contents were significantly higher when higher concentration of fungicide was applied to the crop.

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“…Nucleic acids were fractionated after the principle of Schmidt & Thannhauser (25). DNA was determined according to Burton (9) and RNA was measured as described by Ceriotti (1 2).…”

Section: Anatytical Merhodsmentioning

confidence: 99%

Macrophages and the Synthesis of Connective Tissue Components

Kulonen

Potila

1980

Acta Pathologica Microbiologica Scandinavica Section C Immunolo

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Kulonen, E. & Potila, M. Peritoneal macrophages and the synthesis of connective tissue components. Acta path. microbiol. scand. Sect. C. 88: 7-1 3, 1980. I . Macrophage homogenate, added to the incubation medium of granulation tissue slices, decreases the incorporation of labelled proline to collagen and other proteins, to a greater extent in the presence of certain prostaglandins and in oxygen atmosphere, but to a lesser degree if serotonin and cyclic AMP are added. 2. Rheumatoid synovial fluid, connective tissue active peptide (CTAP) of Castor and an extract from CClcdamaged liver stimulate collagen synthesis in the presence of macrophages. 3. Preparations from non-treated macrophages stimulate the incorporation of glucosamine to acid mucopolysaccharides, mainiy hyaluronate. 4. In a developing rat granuloma, the formation of collagen was stimulated by homogenized macrophages and by macrophage culture medium, but especially by whole macrophages in the presence of Si02.This study was inspired by the finding that SiOz releases from macrophages a nfibrogenic factor({ ' which stimulates protein synthesis in incubated granulation-tissue slices (1) and in cell-free polyso-ma1 systems (4). Since Si02 and related minerals

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A Method for the Determination of Desoxyribonucleic Acid, Ribonucleic Acid, and Phosphoproteins in Animal Tissues

Cited by 2,417 publications

References 6 publications

Anatomical, Physiological and biochemical studies of the cerebellum fromreelermutant mouse

Anatomical, Physiological and biochemical studies of the cerebellum fromreelermutant mouse

Topsin-M application affected nucleic acid, amino acid and phenol contents of Cicer arietinum L

Macrophages and the Synthesis of Connective Tissue Components

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