A method for the determination of N‐nitrosodiethanolamine in personal care products – collaboratively evaluated by the CTPA Nitrosamines Working Group

Abstract

A procedure for the determination of N-nitrosodiethanolamine (NDELA) in personal care products was evaluated in collaborative studies by member organizations of the United Kingdom's Cosmetic Toiletry and Perfumery Association (CTPA) and LGC Limited, formerly known as the Laboratory of the Government Chemist (LGC). Samples were prepared depending on the matrix of the cosmetic product: aqueous samples were prepared by diluting in water followed by solid-phase extraction; emulsions, oils and solid materials were dissolved in dichloromethane and extracted with water. NDELA was separated from the sample matrix using reverse-phase liquid chromatography. The N-nitroso bond was cleaved by photolysis to give nitrite, which was colorimetrically quantified. The nitrite functional group reacted with sulphanilamide in an acid medium to form a diazonium ion which was then coupled with N-(1-naphthyl)ethylenediamine dihydrochloride according to the Griess reaction to give a purple-coloured azo dye that absorbed at 540 nm. Compared with other published methods for NDELA, the method described here is quick and easy to use. It has the required sensitivity and specificity, and can accurately and reliably quantify NDELA in a wide range of personal care product matrices.

Fully automated determination of N‐nitrosamines in environmental waters by headspace solid‐phase microextraction followed by GC–MS–MS

“…N ‐Nitrosamines, especially N ‐nitrosodimethylamine (NDMA), are a group of compounds derived from amines that can have mutagenic, teratogenic, and carcinogenic effects in humans and a wide variety of animals 1. They have been found in many food products 2, beer 3, cosmetics 4, 5, tobacco smoke 6, soils 7, water from chlorinated swimming pools 8, wastewater, treated wastewater, groundwater, and drinking water 8, 9. Polymers, plasticisers, batteries, rocket fuel (incomplete oxidation of hydrazines), and other industrial products are the main anthropogenic sources of the N ‐nitrosamines 10.…”

Evaluation of Level of Precursors of N-Nitrosamine in Vitro in Wistar Rats Fed Different Levels of Dietary Protein

“…23 They can be found in contaminated food, feeding stuff (where they create the highest risk for health), drugs, cosmetics products and pesticides. 24,25 Nitrosamines are absorbed by skin, airways and the alimentary tract.…”

Determination of N‐nitrosamines in cosmetic products by vortex‐assisted reversed‐phase dispersive liquid–liquid microextraction and liquid chromatography with mass spectrometry

“…Most of them are dedicated to the determination of N‐ nitrosodiethanolamine (NDELA), which is the most frequently detected . LC is the most chosen analytical technique, either coupled with thermal energy analyzer (TEA) , UV–Vis , MS/MS , or differential pulse polarography . Besides, GC either coupled with TEA , MS , electrolytic conductivity detector , or electron capture detector has also been employed for its determination.…”