A Method for the Detection of Changes in Gelatin Due to Bacteria: Two Plates

Frazier, W. C.

doi:10.1093/infdis/39.4.302

Cited by 194 publications

(89 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The responses of the fish, "other animal" and human groups to elastin were very close and significantly higher than that of the water group (Table 6). usually used to determine the proteolytic activity of bacterial organisms: 1) gelatin liquefaction based upon hydrolysis of gelatin in tubed medium (COWAN and STEEL, 1965); 2) an assay which detects either gelatin, casein , elastin or milk hy drolysis in a plate medium (FRAZIER , 1926;MARTLEY et al, 1970;SCHARMANN , 1972;SCHMMACHER and SCHILL, 1972;SMITH, 1946;SOKOL et al, 1979;VON RIESEN, 1975); 3) an enzymatic assay which detects the proteolytic ac tivity in a cultured broth using casein, azocasein or hemoglobin as a substrate (CHARNEY and TOMARELLI, 1974;DRAPEAU et al, 1972;REIMERDES and KLOSTERMEYER, 1976). The gelatin liquefac tion and plate assay are usually used for qualita tive studies.…”

Section: Relationship Of Proteolytic Activitiesmentioning

confidence: 99%

Extracellular proteolytic activity of Aeromonas hydrophila complex.

1985

View full text Add to dashboard Cite

A simple quantitative plate assay was used to study the proteolytic activity of Aeromonas hydrophila complex. All the A. hydrophila complex strains hydrolyzed albumin, casein, and fibrinogen; most of the strains also digested gelatin (99.9 %), hemoglobin (94.3%), and elastin (73.2 %). None of the strains hydrolyzed collagen. The activity on each substrate varied from isolate to isolate. By using correlation analysis a close relationship was obtained among these proteolytic reactions, especially with albumin, casein, fibrinogen, gelatin, and hemoglobin hy drolysis. The elastin hydrolysis demonstrated a lower correlation with the other 5 proteolytic activities and implied a different enzymatic system. A higher casein and elastin hydrolytic re sponse was found in the strains derived from human, fish, and other animal sources than those from water environments.

show abstract

Section: Relationship Of Proteolytic Activitiesmentioning

confidence: 99%

Extracellular proteolytic activity of Aeromonas hydrophila complex.

1985

View full text Add to dashboard Cite

show abstract

“…Gelatin hydrolysis was observed on plates of yeast glucose agar with 0.40/0 gelatin (Frazier, 1926).…”

Section: Bees and Honeymentioning

confidence: 99%

Gluconic Acid-producing Bacteria from Honey Bees and Ripening Honey

Ruiz-Argüeso

Rodríguez‐Navarro

1973

Journal of General Microbiology

View full text Add to dashboard Cite

show abstract

“…Because of the difficulty of detecting milk-protein digestion in strongly alkaline liquid cultures, the method involving spot inoculation of cultures on to skim-milk agar plates (skim milk, 20 % (v/v); washed agar, 2.0 % (w/v); adjusted to pH 7.2) was also used. After 7 days of incubation the plates were flooded with HgC1, solution (Frazier, 1926), and any clear zones of hydrolysis measured.…”

Section: Pseudomonas Fluorescens 227mentioning

confidence: 99%

The Characterization of Pseudomonas fluorescens

Rhodes

1959

Journal of General Microbiology

184

View full text Add to dashboard Cite

SUMMARY: A study of phytopathogenic pseudomonads was begun, but it was found that they could not easily be differentiated from the commonly occurring soil-and water-inhabiting fluorescent pseudomonads. A collection of 169 isolates, including 24 named cultures from various collections, was studied. Both old and new kinds of diagnostic characters, cytological, physiological and biochemical, were investigated under standard conditions. Recordings were made at frequent intervals during a 28-day incubation period. Each character was investigated a t least three times during a period of at least five years in order to assess its stability.A clear differentiation between Pseudomonas hydrophila (NCTC '7810) and P. icthyosmia (NCTC 8049) and the rest of the collection was apparent; these two isolates belong to the genus A m m a s Kluyver & van Niel. The use of the vibriostatic pteridine derivative 0/129 revealed a close relationship between them and Vibrio icthyodermis PL 1.Of the 169 isolates (including a t least 20 named species) 165 had 26 common definable and stable characters; these are offered for an extended definition of the genus Pseudomonas. A further 43 characteristics were not common to all 165 isolates. No two of these characters were correlated. One isolate had 42/43 positive characters and one had only 4, and there was a very great range of variation. Among 58 selected isolates there were 21 small groups of identical isolates, but the largest number in any group was 5; the remaining 107 isolates each had a different combination of the 43 characters. Even 10 fluorescent isolates, originally obtained from a single plating of 1 loopful of river water, showed 9 different combinations of characters.A small group of 5 isolates appeared to conform with Pseudomonas aeruginosa as defined by Haynes (1951) and Gaby (1955) but many fluorescens-type isolates were closely related when considered on the basis of all the characters investigated.Several of the named phytophathogens showed a close relationship, or apparent identity, with soil and water isolates ; many phytophathogens, however, had a conspicuous number of negative characters, suggesting loss of adaptability in a more selective environment. The 134 soil-and water-type isolates had 11 positive characters, and 9 others almost always positive. On this basis, and adopting the view that all these characters merit equal emphasis (see Tanner, 1918; Sneath, 1957a, b) it is thought that further divisions into subgroups (species?) are not justified, and that these characters should be used to describe accurately P. fluorescens Migula 1894. A definition of this species is suggested.

show abstract

A Method for the Detection of Changes in Gelatin Due to Bacteria: Two Plates

Cited by 194 publications

References 0 publications

Extracellular proteolytic activity of Aeromonas hydrophila complex.

Extracellular proteolytic activity of Aeromonas hydrophila complex.

Gluconic Acid-producing Bacteria from Honey Bees and Ripening Honey

The Characterization of Pseudomonas fluorescens

Contact Info

Product

Resources

About