A method for the assay of insulin by paper chromatography

Development of an aerosol dosage form containing insulin

“…Methods for the in uiuo detection of insulin have been described in the USP and by various workers (7-9). Several chromatographic methods also were reported (10)(11)(12)(13)(14)(15)(16).…”

Insulin Labelled With Radioactive Iodine: Preparation, Purification and Hormonal Properties

“…Plate 2 shows the Chromatographie migration and the corresponding autoradiograph of a mixture of non-radioactive crystalline insulin and [131I]insulin indicating comparable migration of the two components when run in ascending paper chromatograms using the butane-2-ol : 1 % acetic acid (1:1, v/v) solvent system of Fenton (1959). After many paper chromatograms had been run most of the [131I]-insulin samples gave the same Rp value as that of the crystalline insulin, but trailing and an appreciable deposition of radioactivity at the origin was observed on many occasions.…”

The purification of insulins in crude extracts of rat pancreas by two-dimensional chromatography and electrophoresis on paper

“…This was chromatographed overnight in butan-2-ol-aq. 1 % acetic acid (1:1, v/v), as described by Fenton (1959). Crystalline ox insulin was run alongside as a marker.…”