A method for the analysis of 121 multi-class environmental chemicals in urine by high-performance liquid chromatography-tandem mass spectrometry

Zhu, Hongkai; Chinthakindi, Sridhar; Kannan, Kurunthachalam

doi:10.1016/j.chroma.2021.462146

Cited by 27 publications

(19 citation statements)

References 40 publications

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“…In general, the determined values were in good agreement with the reference values of both SRMs especially given the extremely broad scope of the new workflow with recoveries ranging from 74% to 124% in SRM1950 and recoveries between 81% and 115% in SRM3672 (Figure 3C). These recoveries are similar to values reported in literature for SRM3672 by Karthikraj et al (2020) (80.5% -105%) and Zhu et al (2021) (80 -111%). In the reference materials, several additional metabolites and xenobiotics were quantified (Figure S3, Table S16).…”

Section: Limitationssupporting

confidence: 90%

Integrated exposomics/metabolomics for rapid exposure and effect analyses

Flasch

Fitz

Rampler

et al. 2022

Preprint

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Environmental drivers of disease susceptibility, referred to as the exposome in its totality, are poorly understood. Measuring the myriad of chemicals that humans are exposed to is immensely challenging and identifying disrupted metabolic pathways is an even more complex task. Here, we present a novel technological approach for the comprehensive, rapid and integrated analysis of the endogenous human metabolome and the chemical exposome. By combining reverse-phase and hydrophilic interaction liquid chromatography and fast polarity switching, molecules with highly diverse chemical structures can be analyzed in 15 minutes with a single analytical run. Standard reference materials and authentic standards were evaluated to critically benchmark performance. Highly sensitive median limits of detection with 0.04 µM for >140 quantitatively assessed endogenous metabolites and 0.08 ng/mL for the >100 model xenobiotics were obtained. To prove the dual-column approach’s applicability, real-life samples from sub-Saharan Africa (high exposure scenario) and Europe (low exposure scenario) were assessed in a targeted and non-targeted manner. Our LC-HRMS approach demonstrates the feasibility to quantitatively and simultaneously assess the endogenous metabolome and the chemical exposome for the high-throughput measurement of environmental drivers of disease.

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Section: Limitationssupporting

confidence: 90%

Integrated exposomics/metabolomics for rapid exposure and effect analyses

Flasch

Fitz

Rampler

et al. 2022

Preprint

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“… 13 This analytical method is consistent with Centers for Disease Control and Prevention (CDC) methods and was previously validated and applied in a convenience sample of 21 adult nonpregnant volunteers from Albany, NY. 14 − 16 Ours is the first population-based study to apply this method and the first among a diverse pregnant population. Chemical groups, parent compounds, CAS registry numbers, and environmental transformation of included analytes are provided in Data File S1 , Supporting Information 2.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, urine samples were analyzed at WC-HHEAR using solid-phase extraction (SPE) coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) 14 Urine samples (0.5 mL) were incubated with β-glucuronidase/arylsulfatase (2000 units) and subjected to ABS Elut NEXUS SPE (Agilent, Santa Clara, CA) prior to analysis by HPLC-MS/MS. A Sciex HPLC system (SCIEX, Redwood City, CA) interfaced with an ABSCIEX QTRAP 5500+ triple quadrupole mass spectrometer (Applied Biosystems, Foster City, CA) with an electrospray ionization source was used in the analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The optimal LC-MS/MS conditions and quality assurance protocols are described in detail elsewhere. 14 Limits of detection (LODs) are presented in Data File S2 , Supporting Information 2. Due to insufficient resolution to quantify 24 chemicals individually, we quantified 10 composites of multiple chemicals (e.g., we quantified a composite of 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, 3-hydroxyphenanthrene, 4-hydroxyphenanthrene, and 9-hydroxyphenanthrene as a single analyte).…”

Section: Methodsmentioning

confidence: 99%

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Exposure to Contemporary and Emerging Chemicals in Commerce among Pregnant Women in the United States: The Environmental influences on Child Health Outcome (ECHO) Program

Buckley

Kuiper

Bennett

et al. 2022

Environ. Sci. Technol.

Self Cite

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Prenatal chemical exposures can influence maternal and child health; however, few industrial chemicals are routinely biomonitored. We assessed an extensive panel of contemporary and emerging chemicals in 171 pregnant women across the United States (U.S.) and Puerto Rico in the Environmental influences on Child Health Outcomes (ECHO) Program. We simultaneously measured urinary concentrations of 89 analytes (103 total chemicals representing 73 parent compounds) in nine chemical groups: bactericides, benzophenones, bisphenols, fungicides and herbicides, insecticides, organophosphate esters (OPEs), parabens, phthalates/alternative plasticizers, and polycyclic aromatic hydrocarbons (PAHs). We estimated associations of creatinine-adjusted concentrations with sociodemographic and specimen characteristics. Among our diverse prenatal population (60% non-Hispanic Black or Hispanic), we detected 73 of 89 analytes in ≥1 participant and 36 in >50% of participants. Five analytes not currently included in the U.S. biomonitoring were detected in ≥90% of samples: benzophenone-1, thiamethoxam, mono-2-(propyl-6-carboxy-hexyl) phthalate, monocarboxy isooctyl phthalate, and monohydroxy-iso-decyl phthalate. Many analyte concentrations were higher among women of Hispanic ethnicity compared to those of non-Hispanic White women. Concentrations of certain chemicals decreased with the calendar year, whereas concentrations of their replacements increased. Our largest study to date identified widespread exposures to prevalent and understudied chemicals in a diverse sample of pregnant women in the U.S.

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“…The determination of PBs, BPA and its analogs, TCS and BP-3, in human urine have been analyzed by sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) followed by various sample pretreatment approaches [30][31][32][33][34]. However, the elimination of the matrix effect during ionization, an unavoidable phenomenon, using any set of sample pretreatment protocols or chromatographic systems is complex and may cause bias in quantitative data [35,36].…”

Section: Introductionmentioning

confidence: 99%

Determination of Parabens, Bisphenol A and Its Analogs, Triclosan, and Benzophenone-3 Levels in Human Urine by Isotope-Dilution-UPLC-MS/MS Method Followed by Supported Liquid Extraction

Chen

Chang

Sun

et al. 2022

Toxics

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The development of a rapid analytical approach for determining levels of antibacterial agents, plasticizers, and ultraviolet filters in biosamples is crucial for individual exposure assessment. We developed an analytical method to determine the levels of four parabens—bisphenols A (BPA) and its analogs, triclosan (TCS), triclocarban, and benzophenone-3 (BP-3)—in human urine. We further measured the levels of these chemicals in children and adolescents. We used a supported liquid extraction (SLE) technique coupled with an isotope-dilution ultraperformance liquid chromatography-tandem mass spectrometry (ID-UPLC-MS/MS) method to assess the detection performance for these chemicals. Forty-one urine samples from 13 children and 28 adolescents were assessed to demonstrate the capability and feasibility of our method. An acceptable recovery (75.6–102.4%) and matrix effect (precision < 14.2%) in the three-level spiked artificial urine samples were achieved, and good performance of the validated ID-UPLC-MS/MS method regarding linearity, limits of detection, and quantitation was achieved. The within-run and between-run accuracy and precision also demonstrated the sensitivity and stability of this analytical method, applied after SLE. We concluded that the ID-UPLC-MS/MS method with SLE pretreatment is a valuable analytical method for the investigation of urinary antibacterial agents, plasticizers, and ultraviolet filters in humans, useful for human biomonitoring.

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A method for the analysis of 121 multi-class environmental chemicals in urine by high-performance liquid chromatography-tandem mass spectrometry

Cited by 27 publications

References 40 publications

Integrated exposomics/metabolomics for rapid exposure and effect analyses

Integrated exposomics/metabolomics for rapid exposure and effect analyses

Exposure to Contemporary and Emerging Chemicals in Commerce among Pregnant Women in the United States: The Environmental influences on Child Health Outcome (ECHO) Program

Determination of Parabens, Bisphenol A and Its Analogs, Triclosan, and Benzophenone-3 Levels in Human Urine by Isotope-Dilution-UPLC-MS/MS Method Followed by Supported Liquid Extraction

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