A method for studying oxygen diffusion barrier in erythrocytes: effects of haemoglobin content and membrane cholesterol.

Kon, Kazunori; Maeda, Nobuji; Sekiya, Misuzu; Shiga, Tohru; Suda, Takeo

doi:10.1113/jphysiol.1980.sp013528

Cited by 18 publications

(10 citation statements)

References 44 publications

(51 reference statements)

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“…Kon et al 10 studied the kinetics of O 2 egress from human RBC in an apparatus combining a spectrophotometer with a rapid‐response O 2 electrode. They defined a parameter (r) as the ratio of two velocities: V egress (the velocity of O 2 egress into the medium, which was Baker’s yeast) and V diss (the velocity of O 2 dissociation from Hb).…”

Section: Early Studies and Related Reportsmentioning

confidence: 99%

Effect Of Plasma Cholesterol On Red Blood Cell Oxygen Transport

Buchwald

O'Dea

Menchaca

et al. 2000

Clin Exp Pharma Physio

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SUMMARY1. Oxygen (O2) transfer from the blood to tissues is a function of the red blood cell (RBC) O2 saturation (SO2), the plasma O2 content being negligible. Under conditions of increased tissue O2 demand, the SO2 of arterial blood does not change appreciably (97%); however, the SO2 of mixed venous blood, equal to that of the perfused tissues, can go as low as 20%.2. Tissue O2 availability is limited by the exposure time to a RBC, which decreases under conditions of maximum stress (< 1 s). If the O2 unloading time was to increase significantly, because of a decrease in the RBC diffusion constant or an increase in the RBC membrane thickness, the RBC O2 unloading time would exceed tissue (e.g. cardiac) transit time and O2 transfer would be impaired.3. Cholesterol constitutes the non-polar, hydrophobic lipid of the enveloping layer of the RBC membrane. As the cholesterol content of the RBC increases, the fluidity of the membrane decreases and the lipid shell stiffens.4. Early studies demonstrated that high blood cholesterol concentrations were associated with reduced blood O2 transport; in essence, the haemoglobin dissociation curve was shifted to the left. 5. Current investigations have shown that the cholesterol RBC membrane barrier to O2 diffusion delayed O2 entry into the RBC during saturation and delayed O2 release from the RBC during desaturation. In an analysis of 93 patients divided by their cholesterol concentration into five groups, the percentage change in blood O2 diffusion was inversely proportional to the cholesterol concentration.6. The RBC membrane cholesterol is in equilibrium with the plasma cholesterol concentration. It stands to reason that as the plasma cholesterol increases, the RBC membrane becomes impaired and O2 transport is reduced.7. The implications of this new perspective on O2 transport include the ability to increase tissue oxygenation by lowering plasma cholesterol.

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Section: Early Studies and Related Reportsmentioning

confidence: 99%

Effect Of Plasma Cholesterol On Red Blood Cell Oxygen Transport

Buchwald

O'Dea

Menchaca

et al. 2000

Clin Exp Pharma Physio

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“…Phosphate buffers, isosmotic with plasma, or phosphate-buffered saline containing a substantial proportion of phosphate, were often used and are still recommended as suspending media for red cells in studies of hemoglobin function [see, for example, 21,46,49,58,62]. Bookchin et al [4] recently showed that this treatment of the cells results in altered cell properties which were neither intended nor are appropriate for the attempted studies.…”

Section: Formulation Of the Problemmentioning

confidence: 99%

Volume, pH, and ion-content regulation in human red cells: Analysis of transient behavior with an integrated model

1986

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A basic mathematical model of human red cells is presented which integrates the charge and nonideal osmotic behavior of hemoglobin and of other impermeant cell solutes with the ion transport properties of the red cell membrane. The computing strategy was designed to predict the behavior of all measurable variables in time in ways that optimize comparison with experimentally determined behavior. The need and applications of such a model are illustrated in three separate examples covering different areas of experimentation in the physiology and pathophysiology of red cells.

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“…First in (b), the cell volume and the intracellular haemoglobin concentration are known to modify the intracellular oxygen diffusion by altering the diffusion distance and the intracellular viscosity, respectively (Carlsen & Comroe, 1958;Sirs, 1963;Harrington, Elbaum, Bookchin, Wittenberg & Nagel, 1977;Kon et al 1980). Both these factors were found to be similar in the transformed cells and in the normal discocytes.…”

Section: Discussionmentioning

confidence: 99%

“…The light scattering of the erythrocyte suspension was cancelled by dim,-ing the reference beam as described previously (Kon, Maeda, Sekiya, Shiga & Suda, 1980). The reliability for determining the degree of deoxygenation was ascertained as follows.…”

Section: Erythrocyte Deformation and Oxygen Releasementioning

confidence: 99%

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The influence of deformation of transformed erythrocytes during flow on the rate of oxygen release.

Kon¹,

Maeda²,

Shiga³

1983

The Journal of Physiology

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SUMMARY1. The deoxygenation rates of transformed erythrocytes were compared with those ofnormal discocytes by both stopped-flow and continuous-flow methods. Echinocytic and spherostomatocytic transformations were induced by various anionic and cationic drugs, respectively, without altering the oxygen affinity of haemoglobin, the cell volume or the membrane fluidity.2. The echinocytic transformation reduced the deoxygenation rate at slow-flow velocities (50 cm/sec), as detected by the continuous-flow method. However, at higher flow velocities (150 cm/sec) the rate was similar to that seen in normal discocytes. A close correlation between the degree of echinocytosis, the retardation of deoxygenation rate and the increase of suspension viscosity were observed.3. Microscopic observation of flowing erythrocytes revealed that the echinocytes scarcely deformed at the slower flow velocity, but clearly deformed at the higher flow velocity to various shapes resembling the flowing discocytes.4. Transformation to spherostomatocytes had no effect on the deoxygenation rate, which was comparable with that of the discocytes, and even the higher flow force did not induce any deformation.5. The retarded deoxygenation and the increased viscosity of echinocytes was probably due to an augmented stagnant layer around the cells (i.e. an increase of the hydrodynamic effective volume); this layer was reduced when the echinocytes were deformed with increasing flow force.

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A method for studying oxygen diffusion barrier in erythrocytes: effects of haemoglobin content and membrane cholesterol.

Cited by 18 publications

References 44 publications

Effect Of Plasma Cholesterol On Red Blood Cell Oxygen Transport

Effect Of Plasma Cholesterol On Red Blood Cell Oxygen Transport

Volume, pH, and ion-content regulation in human red cells: Analysis of transient behavior with an integrated model

The influence of deformation of transformed erythrocytes during flow on the rate of oxygen release.

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