A Method for Simultaneous Determination of 14 Carbonyl-Steroid Hormones in Human Serum by Ultra High Performance Liquid Chromatography–Tandem Mass Spectrometry

Bai, Ya-Li; Hong, Zhidan; Zhang, Tianyi; Cai, Bingna; Zhang, Yuanzhen; Feng, Yu‐Qi

doi:10.1007/s41664-020-00120-5

Cited by 16 publications

(5 citation statements)

References 26 publications

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“…It also shows higher sensitivity in full scan mode and a higher intensity range than triple quadrupole mass spectrometry and time-of-flight mass spectrometry (TOF MS). 15 − 18 Finally, a rapid and sensitive UHPLC–MS method was considered as one of the most available detection techniques for determination of CGAs.…”

Section: Introductionmentioning

confidence: 99%

“…With the recent progress of mass spectrometry and separation methods, liquid chromatography coupled with tandem mass spectrometry, particularly, UHPLC-Q-exactive Orbitrap mass spectrometry, has acquired considerable attraction for the qualitative and quantitative analysis of phenolic acid compounds, which reveals its remarkable high resolution and separation capability in chemical characterization and affords accurate mass measurement (<5 ppm) for providing evidence in trace analytes in complex matrices and detailed mass spectral information. It also shows higher sensitivity in full scan mode and a higher intensity range than triple quadrupole mass spectrometry and time-of-flight mass spectrometry (TOF MS). − Finally, a rapid and sensitive UHPLC–MS method was considered as one of the most available detection techniques for determination of CGAs.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Quantification of Chlorogenic Acids from the Root Bark of Acanthopanax gracilistylus by UHPLC-Q-Exactive Orbitrap Mass Spectrometry

et al. 2022

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The purpose of this study is to identify and quantify the chlorogenic acids (CGAs) from the root bark of Acanthopanax gracilistylus , which is conventionally regarded as a tonic in folk Chinese Traditional medicine. The effective methods for identification and quantification analysis of CGAs were developed based on ultra high performance liquid chromatography-Q-exactive orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) in parallel reaction monitoring (PRM) and selected reaction monitoring (SIM), which showed high sensitivity and resolution for screening and quantifying compounds. The root bark of A. gracilistylus was extracted under ultrasonication with 70% methanol. Ultimately, a for total of 70 CGAs, 64 of these were tentatively identified for the first time. Moreover, a methodological study of seven kinds of CGAs was carried out. The proposed procedure was optimized and validated in terms of selectivity, linearity of analytical curves ( r 2 > 0.990), accuracy (recovery range from 96.7 to 105%), and repeatability (relative standard deviation <5%). Then it was applied to determine the content of the CGAs in A. gracilistylus roots from 66 of different batches. The total CGAs was quantified in a range between 2.150 and 33.51 mg/g, which could be considered as excellent source of natural bioactive compound. The result was extremely useful for understanding the bioactive substance and quality control of A. gracilistylus in depth.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of Chlorogenic Acids from the Root Bark of Acanthopanax gracilistylus by UHPLC-Q-Exactive Orbitrap Mass Spectrometry

et al. 2022

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“…To determine 14 carbonyl-steroid hormones in human serum samples after protein precipitation, 2-hydrazinopyridine was used as a derivatization reagent. The correlation coefficient (R 2 ) of the developed method was in the range of 0.9885 to 0.9998, and LOD was in the range of 0.07 to 65.25 ng/ml [45]. Doping analysis has been recognized as a challenge for testing laboratories.…”

Section: Protein Precipitation (Pp)mentioning

confidence: 99%

Estimation of Steroid Hormones in Biological Samples Using Micro Extraction and Advanced Chromatography Techniques

Srivastava¹,

Godbole²,

Shrivastava³

2022

austinjanalpharmchem

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Steroid profiling plays an important role in the clinical setting and also in the diagnosis of various physiological disorders. Steroids in the research laboratory and patient care has been routinely measured by immunoassay. Limitations of immunoassay in quantifying steroids have been well documented and the advent of advanced mass spectrometry is offering a viable alternative to measure multiple steroids in single reaction. Analytes ranging from the steroid and their metabolites that are present in the body carry out various important functions and are relevant to maintaining homeostasis. Blood plasma and urine samples are the clinical material for such analysis, however, due to the difference in polarity of the target steroid, a major challenge exists as to how faithfully analyze different steroids in the given sample type. These steroids are usually present at very low concentrations in the body and are present in several forms leading to increased complexity. Achieving an excellent chromatographic separation for the analysis of steroids requires an effective sample preparation and analysis procedure. Hence various methods are developed to analyze the presence of steroids and to know their exact concentration in the body. This review covers various analytical methodologies such as chromatographic procedures and downstream mass spectrometry for the identification and measurement of the levels of steroid hormones. At the same time, this review also covers the most recent developments that have taken place in the recent past to cover the enhanced understanding of steroid hormone analysis.

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“…Such approaches, including the conversion of carbonyl groups by reaction with hydroxylamine or 2-hydrazinopyridine, were proposed for the highly sensitive determination of endosteroids by HPLC/ESI-MS and UHPLC/ESI-MS [ 101 ], respectively. We can also note a preliminary modification of carbonyl groups with the introduction of a fixed charge (Girard’s reagent), carried out to detect and determine off-target steroid metabolites by a combined method of LC with high-resolution MS (LTQ-Orbitrap) [ 102 ]. In another case, the principles of steroidomics based on UHPLC in combination with high-resolution MS (QTOF) were involved in the process of simultaneous identification and profiling of large arrays of “exogenous” metabolites formed from anabolic steroids as a method of doping control [ 103 ].…”

Section: Mass Spectrometry In Omics Life Sciencesmentioning

confidence: 99%

Mass Spectrometry as a Crucial Analytical Basis for Omics Sciences

Заикин

Борисов

2021

J Anal Chem

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This review is devoted to the consideration of mass spectrometric platforms as applied to omics sciences. The most significant attention is paid to omics related to life sciences (genomics, proteomics, meta-bolomics, lipidomics, glycomics, plantomics, etc.). Mass spectrometric approaches to solving the problems of petroleomics, polymeromics, foodomics, humeomics, and exosomics, related to inorganic sciences, are also discussed. The review comparatively presents the advantages of various principles of separation and mass spectral techniques, complementary derivatization, used to obtain large arrays of various structural and quantitative information in the mentioned omics sciences.

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A Method for Simultaneous Determination of 14 Carbonyl-Steroid Hormones in Human Serum by Ultra High Performance Liquid Chromatography–Tandem Mass Spectrometry

Cited by 16 publications

References 26 publications

Identification and Quantification of Chlorogenic Acids from the Root Bark of Acanthopanax gracilistylus by UHPLC-Q-Exactive Orbitrap Mass Spectrometry

Identification and Quantification of Chlorogenic Acids from the Root Bark of Acanthopanax gracilistylus by UHPLC-Q-Exactive Orbitrap Mass Spectrometry

Estimation of Steroid Hormones in Biological Samples Using Micro Extraction and Advanced Chromatography Techniques

Mass Spectrometry as a Crucial Analytical Basis for Omics Sciences

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