A method for simultaneous analysis of phytosterols and phytosterol esters in tobacco leaves using non aqueous reversed phase chromatography and atmospheric pressure chemical ionization mass spectrometry detector

Ishida, Naoyuki

doi:10.1016/j.chroma.2014.03.021

Cited by 23 publications

(22 citation statements)

References 33 publications

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“…Since deterioration of the APCI corona cone was expected to occur owing to accumulative exposure of a large amount of components derived from replicated analyses as reported (17), RSD (%) of entities obtained from consecutively or intermittently (2, 20, 60, 100) replicated analyses (three times) of a sample was weighed as shown in Figure 4.…”

Section: Methods Validation For Comprehensive Analysismentioning

confidence: 99%

“…Since tobacco leaves were extracted in the same manner and the extracts were separated by the same chromatography as reported (13,15,17), only the parameters applied to MSD for detection were optimized to obtain desirable ion intensity of solanesols (13), triacylglycerols (15) and phytosterols (17). Although chlorophylls in tobacco leaf were originally extracted by acetone (14), as the compositions of both extracts did not show a large difference, n-hexane was used for the analysis to consolidate extraction procedures.…”

Section: Methods Development For Simultaneous Detectionmentioning

confidence: 99%

“…Components for identification were purchased and prepared by separation and synthesis as described in previous reports (13)(14)(15)17).…”

Section: Methodsmentioning

confidence: 99%

“…Compared to EI and ESI, atmospheric pressure chemical ionization (APCI) has still remained a rare interface due to its specificity in low polar components (21,29). Nevertheless the usefulness of APCI-MSD has already been presented by target analysis of non-volatile components with low polarity (13)(14)(15)17) and the application to metabolomics was expected to provide another comprehensive method and new correlation of differences in components with differences in tobacco leaves. The objective of this study was therefore to develop a comprehensive analytical method using LC/APCI-MSD hyphenated with non-aqueous reversed-phase chromatography (NARPC) to correlate differences in chemical profiles with differences in tobacco leaves.…”

Section: Introductionmentioning

confidence: 99%

“…However, the methodologies applied to those studies has employed only gas chromatography mass spectrometry detection (GC/MSD) oriented to volatile components (8,9), or liquid chromatography electrospray ionization mass spectrometry detection (LC/ESI-MSD) for components with middle to high polarity (11). Since non-volatile components with low polarity such as solanesols (12,13), chlorophyll derivatives (14), triacylglycerols (15), solanachromene (16) and phytosterols (17) (Figure 1) are also included in tobacco leaf and have been considered unsuitable for detection in using the previously employed instruments (8)(9)(10)(11), the metabolomics applied to tobacco leaves has yet to cover all the components in tobacco leaves. In the first place, comprehensive analyses for metabolomics consist of a separation and detection part which are hyphenated so as to maximize comprehensiveness of method (7,(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

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A Simultaneous Analytical Method to Profile Non-Volatile Components with Low Polarity Elucidating Differences Between Tobacco Leaves Using Atmospheric Pressure Chemical Ionization Mass Spectrometry Detection

Ishida

2016

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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SUMMARYA comprehensive analytical method using liquid chromatography atmospheric pressure chemical ionization mass spectrometry detector (LC/APCI-MSD) was developed to determine key non-volatile components with low polarity elucidating holistic difference among tobacco leaves. Nonaqueous reversed-phase chromatography (NARPC) using organic solvent ensured simultaneous separation of various components with low polarity in tobacco resin. Application of full-scan mode to APCI-MSD hyphenated with NARPC enabled simultaneous detection of numerous intense product ions given by APCI interface. Parameters for data processing to filter, feature and align peaks were adjusted in order to strike a balance between comprehensiveness and reproducibility in analysis. 63 types of components such as solanesols, chlorophylls, phytosterols, triacylglycerols, solanachromene and others were determined on total ion chromatograms according to authentic components, wavelength spectrum and mass spectrum. The whole area of identified entities among the ones detected on total ion chromatogram reached to over 60% and major entities among those identified showed favorable linearity of determination coefficient of over 0.99. The developed method and data processing procedure were therefore considered feasible for subsequent multivariate analysis. Data matrix consisting of a number of entities was then subjected to principal component analysis (PCA) and hierarchical clustering analysis. Cultivars of tobacco leaves were distributed far from each cultivar on PCA score plot and each cluster seemed to be characterized by identified non-volatile components with low polarity. While fluecured Virginia (FCV) was loaded by solanachromene, phytosterol esters and triacylglycerols, free phytosterols and chlorophylls loaded Burley (BLY) and Oriental (ORI) respectively. Consequently the whole methodology consisting of comprehensive method and data processing procedure proved useful to determine key-components among cultivars of tobacco leaves, and was expected to additionally expand coverage that metabolomics study has ensured. [Beitr. Tabakforsch. Int. 27 (2016)

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Section: Methods Validation For Comprehensive Analysismentioning

confidence: 99%

Section: Methods Development For Simultaneous Detectionmentioning

confidence: 99%

“…Components for identification were purchased and prepared by separation and synthesis as described in previous reports (13)(14)(15)17).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Simultaneous Analytical Method to Profile Non-Volatile Components with Low Polarity Elucidating Differences Between Tobacco Leaves Using Atmospheric Pressure Chemical Ionization Mass Spectrometry Detection

Ishida

2016

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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Guidelines for extraction and quantitative analysis of phytosterols and oxidation products

Yang

Zhao

Liu

et al. 2023

eFood

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Phytosterols (PS) are widely distributed in the plant source foods, and research on their health benefits has become increasingly active. This article briefly outlines the main extraction processes of PS and instrumental analysis methods of PS in detail. The PS isolation technique depends on the nature of the matrix and the form of the PS (free, esterified, and glycosylated). Conventional extraction technologies for PS commonly used in practice were Soxhlet extraction and maceration method. Due to their inherent molecular structure, PS exhibits poor stability to heat, light, oxygen, pH, and metal ions. It is of great significance to find a reliable analytical technique to extract PS and oxidation products from food substances and an accurate detection method of PS in different foods due to the instability of plant sterol and the interference of complex plant‐based matrices. Generally, it is common to use GC–MS to determine the composition of total PS and their oxidation products, which requires standard monomer PS. It is desirable to use LC–MS to determine free PS in liquid samples. These methodologies could be meaningful in the quality assessment, health function evaluation, and applications and limitations of plant‐sourced foods.

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Influence of acid hydrolysis, saponification and sample clean‐up on the measurement of phytosterols in dairy cattle feed using GC–MS and GC with flame ionization detection

Duong

Strobel

Buddhadasa

et al. 2018

J of Separation Science

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The fortification of processed foods including dairy products is increasingly commonplace with phytosterols among many compounds used to improve the nutritional value of food products. It is also increasingly common practice for some dairy cattle feeds to be fortified for their potential to increase phytosterol levels in milk. In this study, a combined, streamlined protocol using acid hydrolysis, saponification and sample clean-up was developed to enable the rapid and reliable measurement of phytosterols. The method was developed with focus on streamlining the overall technique to make it suitable for commercial laboratories, to reduce labor and consumable costs, while maintaining accuracy. A total of 12 different feed types commonly used in the dairy industry were analyzed with the highest and lowest sterol contents found in cotton seed oil and tannin with average phytosterol contents of 256 and <30 mg per 100 g, respectively. With a limit of reporting of 30 mg/kg for individual sterols and a correlation coefficient > 0.99, the method was validated for milk to enable feed comparison studies with respect to the total phytosterol content in raw milk.

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A method for simultaneous analysis of phytosterols and phytosterol esters in tobacco leaves using non aqueous reversed phase chromatography and atmospheric pressure chemical ionization mass spectrometry detector

Cited by 23 publications

References 33 publications

A Simultaneous Analytical Method to Profile Non-Volatile Components with Low Polarity Elucidating Differences Between Tobacco Leaves Using Atmospheric Pressure Chemical Ionization Mass Spectrometry Detection

A Simultaneous Analytical Method to Profile Non-Volatile Components with Low Polarity Elucidating Differences Between Tobacco Leaves Using Atmospheric Pressure Chemical Ionization Mass Spectrometry Detection

Guidelines for extraction and quantitative analysis of phytosterols and oxidation products

Influence of acid hydrolysis, saponification and sample clean‐up on the measurement of phytosterols in dairy cattle feed using GC–MS and GC with flame ionization detection

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