A method for separating according to mass a mixture of macromolecules or small particles suspended in a fluid, 3. Experiments in a centrifugal fluid.

Berg, Howard C.; Purcell, Edward M.

doi:10.1073/pnas.58.5.1821

Cited by 23 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The scalings α 1 = 1 and α 2 = 3 used above are physically motivated, as they belong to experimentally-demonstrated sub-techniques of FFF. Flow-FFF [37,39,66,67], thermal-FFF [41,68,69] and electrical-FFF [27,70,71] each have a force that scales as α i = 1, while sedimentation-FFF [25,72] (including gravitational-FFF [23,73]) is the most obvious commonly-used technique with α i = 3. External forces with scaling of α i = 2 are not commonly utilized by FFF apparatuses.…”

Section: Adverse-mode: Retention Ratio Peakmentioning

confidence: 99%

“…In all of these cases, the adverse device retention parameter has an order of magnitude Λ 1 ∼ −10 −6 . Through Equation (25), the peak position is then predicted to occur at submicron scales, r L = h (−Λ 2 /Λ 1 ) 1/2 ∼ 0.1 µm.…”

Section: Implementation and Feasibilitymentioning

confidence: 99%

“…The flexibility of FFF results from the large number of potential transverse external fields that can be used. Gravitational [23,24], centrifugal sedimentation [25,26], electrical [27,28], magnetic [29,30], dielectrophoretic [31,32], acoustic [33,34], photophoretic [35,36], cross-flow (both symmetrical [37,38] and asymmetrical [39,40]) and thermal [41,42] fields have all been utilized by FFF. Cross-flows (both symmetrical and asymmetrical), sedimentation and thermal gradients are the most commonly-employed fields.…”

Section: Introductionmentioning

confidence: 99%

“…Solid lines show the full theory (Equation(5)), while dashed lines show the near-peak approximation (Equation (26)). Dots markr L from Equation(25).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Adverse-Mode FFF: Multi-Force Ideal Retention Theory

Shendruk¹,

Slater

2015

Chromatography

View full text Add to dashboard Cite

A novel field-flow fractionation (FFF) technique, in which two opposing external forces act on the solute particles, is proposed. When the two external forces are sufficiently strong and scale differently as a function of the solutes' property of interest (such as the solute particle size), a sharp peak in the retention ratio (dramatic drop in elution time) is predicted to exist. Because the external forces oppose one another, we refer to this novel technique as adverse-mode FFF. The location of this peak is theoretically predicted and its ideal width estimated. The peak can become quite sharp by simultaneously increasing the strength of both fields, suggesting that adverse-mode FFF could be a useful technique for accurately measuring single species solute size.

show abstract

Section: Adverse-mode: Retention Ratio Peakmentioning

confidence: 99%

Section: Implementation and Feasibilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Solid lines show the full theory (Equation(5)), while dashed lines show the near-peak approximation (Equation (26)). Dots markr L from Equation(25).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Adverse-Mode FFF: Multi-Force Ideal Retention Theory

Shendruk¹,

Slater

2015

Chromatography

View full text Add to dashboard Cite

show abstract

“…This phenomenon can be used to effect a separation between particles of different size, shape, or density. This separation concept was developed independently by Berg and Purcell (1)(2)(3) and by Giddings (4-6), who termed the method "field-flow fractionation." Here, the theoretical analysis is improved upon and extended to include nonspherical particles in an orienting field.…”

Section: Introductionmentioning

confidence: 99%

Field-Flow Fractionation: Extensions to Nonspherical Particles and Wall Effects

Gajdos

Brenner

1978

Separation Science and Technology

View full text Add to dashboard Cite

A rigorous analysis of the phenomenon of field-flow fractionation (FFF) is presented for particles which are both nonspherical in shape and of sufficient size (compared with apparatus dimensions) to be significantly influenced by wall effects. Calculations are presented for axially-symmetric particles in an arbitrary flow field. Orienting torques directed along the symmetry axis of the particle are also considered. The theory is compared with the experimental data of Berg, Purcell, and Stewart. Reasonably satisfactory agreement is observed.

show abstract