A method for rearing Pecten maximus larvae in the laboratory

Gruffydd, Ll. D.; Beaumont, Andy R.

doi:10.1007/bf00401395

Cited by 80 publications

(57 citation statements)

References 6 publications

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“…Pecten m a x~m u s larvae were raised according to procedures described by Buestel et al (1982), based on work by Loosanoff & Davis (1963) and Gruffydd & Beaumont (1972). For metamorphosis assays, larvae were sorted by screening on a 150 ym mesh.…”

Section: Methodsmentioning

confidence: 99%

Chemical induction of larval metamorphosis of Pecten maximus with a note on the nature of naturally occurring triggering substances

Chevolot¹,

Cochard²,

Yvin³

1991

Mar. Ecol. Prog. Ser.

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ABSTRACT-Delayed metamorphosis usually occurs when scallop Pecten maximus (L.) larvae are reared in still water Metamorphosis rates may b e increased by adding aqueous extracts of certain marine red algae, including one [Delesseria sangulnea (Linne) Lamouroux (Delesseriaceae, Rhodomelaceae)] from which various chemical inducers have been isolated. Of these, jacaranone (a simple quinol) is by far the most active component; other components (p-OH phenylacetic acid methyl ester, p-OH benzaldehyde, p -O H benzyl alcohol) are only weak inducers. All are catabolic derivatives of tyrosine. Homogentisic acid (biosynthetically related to jacaranone) and some catecholamines (L-DOPA, epinephrine) can also induce metan~orphosis of P. maximus larvae. All these con~pounds can be transformed into quinones by oxidation. This result, together with literature data, strongly suggests that the true inducer(s) present in the envuonment might possess a quinone or quinone-like structure, as IS the case for jacaranone.

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Section: Methodsmentioning

confidence: 99%

Chemical induction of larval metamorphosis of Pecten maximus with a note on the nature of naturally occurring triggering substances

Chevolot¹,

Cochard²,

Yvin³

1991

Mar. Ecol. Prog. Ser.

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“…This suggests that the sperm-to-egg ratio of 1:1 to 5:1 previously reported by Gruffydd and Beaumont (1972) could be an underestimate for great scallop. A 2.5 fold decrease of the fertilization capacity of great scallop sperm was observed in the thawed batch compared with fresh sperm, probably reflecting the lower number of motile thawed sperm relative to the control.…”

Section: Discussionmentioning

confidence: 69%

Cryopreservation of great scallop (Pecten maximus) sperm: effect of extender, cryoprotectant and cooling rate on sperm survival

Suquet

Arsenault-Pernet

Ratiskol

et al. 2014

Aquat. Living Resour.

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-To develop selection programs for the great scallop, artificial reproduction of the species needs to be improved by gamete cryobanking. Here, a set of four experiments was designed in order to define the basic elements of a cryopreservation protocol for scallop sperm, including extender composition (experiment 1), cryoprotectant selection (2), cooling rate (3), and assessment of the effects of sperm cryopreservation on sperm motility and fertilization capacity (4). Sperm was collected after serotonin injection (100 µl of a 10-mM solution) and frozen in 500-µl straws. For the first three experiments, the percentage of motile fresh sperm (80 ± 4%, mean ± SEM) was significantly higher than that observed for thawed sperm (11 ± 2%). During the first experiment, no significant difference of the percentage of motile thawed sperm was observed among the three saline extenders tested: seawater, calcium-free Hanks' balanced salt solution (Ca-free HBSS) and DCSB4 solution. However, a complementary experiment (2) showed that a significantly higher percentage of motile thawed sperm was recorded using DCSB4 than with Ca-free HBSS as an extender. During the third experiment, sperm motility was higher when using polyethylene glycol (10% PEG) as a cryoprotectant, than when using dimethyl sulphoxide, DMSO (10 and 20%), methanol (10%) and ethylene glycol (10% EG). A higher D-larval rate was obtained during the fourth experiment, using fresh sperm than thawed sperm, and using a 500:1 sperm-to-egg ratio compared with a 50:1 ratio. There is some evidence of inter-individual variations in sperm tolerance to cryopreservation. In conclusion, the highest survival of great scallop sperm after thawing was recorded using the following conditions: DCSB4 extender (1:3 vol/vol sperm to extender dilution), PEG cryoprotectant (10%) and straws maintained at 5.5 cm above liquid nitrogen.

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“…Increasing scallop collection with extended periods of immersion of collectors has also been reported by Román and Cano (1987). In contrast to these results, however, various authors have suggested that for pectinids, including Placopecten magellanicus, P. maximus and P. yessoensis, extended periods of immersion of collectors only results in the biofouling of the collectors, inhibiting larval settlement and interference with the survival of settled scallops (Yamamoto, 1964;Minchin, 1976;Gruffydd and Beaumont, 1972;Gruffydd et al, 1975;Paul, 1978;Naidu and Scaplen, 1979;Brand et al, 1980;Fegan, 1983;Thouzeau, 1989;1991a, b). Fegan (1983), suggested that biofouling of the collectors became effective in inhibiting scallop settlement after 15 days, and Wilson (1987), failed to observe settlement of P. maximus after one month of collector immersion.…”

Section: Discussionmentioning

confidence: 96%

Artificial collection and early growth of spat of the scallop <i>Argopecten purpuratus</i> (Lamarck, 1819), in La Rinconada Marine Reserve, Antofagasta, Chile

Avendaño¹,

Cantillánez²,

Thouzeau³

et al. 2007

Sci. Mar.

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SUMMARY: Artificial collection of early juveniles ("spat") of the scallop Argopecten purpuratus in Japanese-type collectors was evaluated between January 2001 and July 2002 in the La Rinconada Marine Reserve, Antofagasta, Chile. This area of Antofagasta Bay has in the past been noted for the retention of scallop larvae by local gyres, in which their numbers can vary between 89 and 34175 larvae m -3 , producing larval sets of 400 to 15340 post-larvae (spat) per collector. The results showed no quantitative relationship between larval abundance in the water and the spat density collected per day in the collectors, although high settlement rates were associated with high numbers of umboned larvae in the water. Allowing collectors to remain in situ for extended periods of 88 and 159 days resulted in a severe loss of seed which had settled in the collectors during the first 28 to 40 days of immersion. These losses varied between 50.9 and 99.6% of the spat collected, and were more prejudicial for the smaller cohorts that had settled in the collectors at the end of the first immersion period. The growth rates measured among different cohorts for each immersion period varied between 81.3 and 235.2 μm/day for the first cohort (C 1 ) and between 64.0 and 167.4 μm/day for the second cohort (C 2 ). The highest growth rates occurred in collectors containing the lowest numbers of spat after the occurrence of spat losses during the long periods of immersion. Occurrence of intraspecific competition within the collectors is discussed as potentially responsible for the decreases in spat numbers and the variations observed in their growth rates. No se encontró una relación directa entre abundancia larvaria y asentamiento, sin embargo, las mayores captaciones estuvieron asociadas a la presencia de un elevado número de estados umbonados. La mantenimiento prolongado de colectores in situ, por periodos que variaron entre 88 y 159 días, provocó una fuerte reducción en el número de semillas que se asentaron en ellos durante los primeros 28 a 40 días de inmersión Estas reducciones variaron entre el 99.6 y el 50.9% de la semilla fijada, siendo las más perjudicadas las cohortes más pequeñas, asentadas al final del primer periodo de inmersión. Las tasas de crecimiento registradas en estas cohortes, para cada periodo de inmersión, variaron entre 81.3 y 235.2 μm/día para las cohortes C 1 y entre 64.0 y 167.4 μm/día para las cohortes C 2 . Las mayores tasas están directamente relacionadas con un menor número de semilla recuperada. Se discuten las competencias intraespecíficas ocurridas en los colectores, como responsables de la disminución del número de semilla y de las variaciones registradas en sus tasas de crecimiento.

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A method for rearing Pecten maximus larvae in the laboratory

Cited by 80 publications

References 6 publications

Chemical induction of larval metamorphosis of Pecten maximus with a note on the nature of naturally occurring triggering substances

Chemical induction of larval metamorphosis of Pecten maximus with a note on the nature of naturally occurring triggering substances

Cryopreservation of great scallop (Pecten maximus) sperm: effect of extender, cryoprotectant and cooling rate on sperm survival

Artificial collection and early growth of spat of the scallop <i>Argopecten purpuratus</i> (Lamarck, 1819), in La Rinconada Marine Reserve, Antofagasta, Chile

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