A Method for Real-Time Assessment of Mitochondrial Respiration Using Murine Corneal Biopsy
Wentao Liang,
Li Huang,
Tian Yuan
et al.
Abstract:Purpose
To develop and optimize a method to monitor real-time mitochondrial function by measuring the oxygen consumption rate (OCR) in murine corneal biopsy punches with a Seahorse extracellular flux analyzer.
Methods
Murine corneal biopsies were obtained using a biopsy punch immediately after euthanasia. The corneal metabolic profile was assessed using a Seahorse XFe96 pro analyzer, and mitochondrial respiration was analyzed with specific settings.
Resul… Show more
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