A method for production of Nα-benzyloxycarbonyl-aminoadipate-δ-semialdehyde with amine oxidase from Aspergillus niger

Enzymes responsible for metabolism of Nα-benzyloxycarbonyl-l-lysine in microorganisms

“…It was reported earlier that A. niger AKU 3302 produced amine oxidase (MAO, EC 1.4.3.6), which catalyzed oxidation of aliphatic and aromatic amines but not amino acids [7]. However, recently Isobe et al revealed that N-Z-L-lysine was also oxidized by the MAO, although L-lysine was not oxidized [3]. Thus, it was revealed that the oxidase from A. niger…”

“…Isobe et al [3] revealed that MAO from A. niger strain oxidized not only N-Z-L-lysine but also N-Z-D-lysine. In contrast, L-AAO from Rhodococcus strain oxidized N-Z-L-lysine and L-amino acids, but did not oxidize N-Z-D-lysine and D-amino acids.…”

“…Recently, simple biochemical methods for selective production of N-Z-L-AASA and N-Z-L-AAA were also reported using A. niger AKU 3302 or Rhodococcus sp. AIU Z-35-1 [3][4][5][6]. In this paper, enzymes…”

Enzymes Responsible for the Conversion of Nα‐[(Benzyloxy)carbonyl]‐D‐lysine to Nα‐[(Benzyloxy)carbonyl]‐D‐aminoadipic Acid by Rhodococcus sp. AIU Z‐35‐1

“…AIU Z-35-1. We have already revealed in an earlier report [7] that N a -Z-l-lysine and N a -Z-d-lysine were converted to N a -Z-l-AASA and N a -Z-d-AASA, respectively, by amine oxidase from Aspergillus niger AKU 3302. Thus, the enzyme catalyzing the conversion of N a -Z-d-lysine to N a -Z-d-AASA in the Rhodococcus strain was also different from that of A. niger.…”

Microbial resolution of Nɛ-acetyl-dl-lysine with Rhodococcus sp. AIU Z-35-1

“…L--Aminoadipate -semialdehyde (L--AASA) and L--aminoadipic acid (L--AAA) are important precursors in biosynthesis of -lactam antibiotics or L-lysine (1,2). Since those precursors and their related compounds have become interesting raw materials for chemical synthesis of new antibiotics or physiologically active peptides, some chemical and biochemical methods for production of L--AASA, L--AAA or their derivatives have been developed (3)(4)(5)(6)(7)(8)(9). However, those methods were complicated and their conversion yields were low.…”