A Method for Measuring the Biodegradation of Organic Chemicals

Boatman, Rodney J.; Cunningham, Suzanne L.; Ziegler, Donald A.

doi:10.1897/1552-8618(1986)5[233:amfmtb]2.0.co;2

Cited by 2 publications

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“…The main disadvantage of the Sturm test is that it is labour intensive, time consuming and as a consequence replications of the test are difficult (Boatman et al, 1986). The CO, released is trapped as barium carbonate.…”

Section: (4) Assays Based On Co Productionmentioning

confidence: 99%

Treatability, Toxicity and Biodegradability Test Methods

Kilroy

Gray

1995

Biological Reviews

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1. This review confirms that treatability and biodegradability test methods have been cited extensively in the literature. It is clear that the method selected depends on the specific objectives of the test, i.e. the determination of whether a substance is toxic, biodegradable or treatable. Factors that have to be considered when selecting the test methods are the cost of performing the test, the time and resources involved, and the accuracy required. It often appears that more extensive simulation studies are required after initial screening tests have been performed. 2. Many of the enzyme and bacterial growth tests which have been developed for monitoring or screening of toxicants and their persistence in water and wastewaters have been reviewed. Most of these tests are rapid, inexpensive, and reproducible. Most of the biochemical and microcalorimetric approaches, although promising, are still in their infancy as regards toxicity testing. Therefore, biological testing still appears to be most suitable for routine assessment. 3. Micro-organisms are particularly suitable for use in toxicity testing of chemicals as they are inexpensive to culture, have rapid growth rates, and usually provide reproducible results (Vaishnav & Korthals, 1990). Many bioassays have been developed to evaluate the toxicity and treatability of municipal and industrial effluents. Numerous single species tests have been recommended by several authors (Dutka et al., 1983; Beaubien et al. 1986). Such approaches are mainly based on the belief that, by selecting the most sensitive species and by using appropriate factors to allow for variability not included in the test, the highest levels of biological organization will be adequately protected. Single species tests are now quite well established, and when properly used, are easy to analyse and quantify. However, it has been pointed out (Levin, 1984) that the results obtained from single species tests cannot easily be applied to natural field conditions because the test organisms are extensively laboratory acclimated; also the test conditions provide for optimized growth and survival, a situation unlikely to be found in the field. Moreover, a fundamental problem with this approach is that it assumes that the ecosystem is a collection of single species exposed to toxicants under constant conditions (Cairns, 1982). Multi-species toxicity tests, that is the use of mixed cultures or communities of micro-organisms for a testing protocol, are found to be generally much less sensitive than single species tests (Dutka & Kwan, 1984).(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: (4) Assays Based On Co Productionmentioning

confidence: 99%

Treatability, Toxicity and Biodegradability Test Methods

Kilroy

Gray

1995

Biological Reviews

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“…Boatman et al (12) ont réexaminé les méthodes précédentes et adopté un système d'espace libre fermé dans lequel le CO 2 est libéré dans l'espace libre à la fin de l'incubation moyennant une acidification du milieu. Le CO 2 était mesuré par chromatographie gazeuse (carbone inorganique) dans des échantillons prélevés automatiquement de l'espace libre, mais le carbone inorganique dissous dans la phase liquide n'était pas pris en compte.…”

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Essai n° 310 : Biodégradabilité facile - dégagement de CO2 dans des flacons hermétiquement clos (essai de l'espace libre au-dessus du liquide)

2014

Lignes Directrices De l'OCDE Pour Les Essais De Produits Chimiques, Section 3

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A Method for Measuring the Biodegradation of Organic Chemicals

Cited by 2 publications

References 0 publications

Treatability, Toxicity and Biodegradability Test Methods

Treatability, Toxicity and Biodegradability Test Methods

Essai n° 310 : Biodégradabilité facile - dégagement de CO2 dans des flacons hermétiquement clos (essai de l'espace libre au-dessus du liquide)

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