A method for <i>in vivo</i> high resolution MRI of rat spinal cord injury

Ford, John C.; Hackney, David B.; Joseph, Peter M.; Phelan, Megan; Alsop, David C.; Tabor, Stacy L.; Hand, Christopher M.; Markowitz, Ronald S.; Black, Perry

doi:10.1002/mrm.1910310216

Cited by 49 publications

(29 citation statements)

References 13 publications

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“…Surface coils were used to image it at 7.0 T [13], to image and do localized MRS at 4.7 T [14]. Implanted coils were used for imaging at around 2.0 T [15][16][17].…”

Section: System Studiedmentioning

confidence: 99%

MR microscopy of water diffusion tensor in biological systems

et al. 1998

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Diffusion tensor imaging (DTI) of the control and mechanically injured spinal cord of a rat in vitro and in vivo are reported. In vitro experiments were done on a home-built 6.4 T magnetic resonance microscope. Results for formaline-fixed samples show significant differences in diffusion tensor components between gray matter and white matter of the control spinal cord. Moreover, it is shown that already 6-10 min after the injury DTI can detect changes in water diffusion in areas extending far beyond the region of primary tissue damage. In vivo experiments were performed using a 9.4 T Magnex magnet and Bruker Medspec imaging system. Good-quality DTI images, free from motion artifacts were obtained. Results from control samples confirm differences in water diffusion between white matter and gray matter, observed in vitro. In vivo experiments show that characteristic changes in water diffusion observed in vitro 6-10 min after injury are preserved 60-360 min after injury, without significant alteration during this time.

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“…Surface coils were used to image it at 7.0 T [13], to image and do localized MRS at 4.7 T [14]. Implanted coils were used for imaging at around 2.0 T [15][16][17].…”

Section: System Studiedmentioning

confidence: 99%

MR microscopy of water diffusion tensor in biological systems

et al. 1998

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“…An alternative for determining AIF without introducing disruptions in vascular architectures and blood circulation is an implanted RF coil covering an artery of interest. Implanted RF coils have been used for: (1) imaging small anatomical structures (e.g., spinal cord) located in deep regions of the animal body in order to increase signal-to-noise ratio (SNR) over that obtained by a regular exterior RF coil [8,9,10,11,12,13,14,15,16,17,18,19], and (2) imaging vascular walls with an implanted intravascular or extravascular RF coil [20,21]. In this work, we have designed an 17 O implanted RF coil and combined this coil with the in vivo 17 O NMR approach for reliably measuring the AIF of 17 O-labeled water tracer in the rat carotid artery after a H 2 17 O bolus injection.…”

Section: Introductionmentioning

confidence: 99%

Measurement of arterial input function of 17O water tracer in rat carotid artery by using a region-defined (REDE) implanted vascular RF coil

Zhang

Zhu

Tian

et al. 2003

Magn Reson Mater Phy

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A method of determining arterial input function (AIF) by continuously detecting the (17)O MR signal changes of (17)O-labeled water tracer in the rat carotid artery using a region-defined (REDE) implanted vascular RF coil at 9.4 Tesla is reported. This coil has a compact physical size of 1 mm inner diameter, 3 mm outer diameter and 11 mm in length. It can be readily implanted into the rat neck and wrapped around the rat carotid artery for achieving adequate MR detection sensitivity for determining AIF with minimal surgical trauma. Water phantom and in vivo MR experiments were conducted for validating the coil's performance. A signal-to-noise ratio of approximately 20:1 was achieved for the (17)O signal acquired from naturally abundant H(2)(17)O in a small amount of blood (approximately 7 microl) inside the rat carotid artery with an acquisition time of 11 s. The REDE RF coil design electromagnetically isolates the rat carotid artery from surrounding tissues and ensures that the MR signal detected by the RF coil is only attributable to the artery blood. It also minimizes the electromagnetic coupling between the implanted RF coil and a head surface coil tuned at the same operating frequency (two-coil configuration). This configuration allowed simultaneous measurements of dynamic changes of (17)O MR signal of the H(2)(17)O tracer in both rat carotid artery and brain. Compared to most contemporary MR approaches, the REDE implanted RF provides a simple, accurate, and promising solution for determination of AIF in small experimental animals.

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“…This provides a quantitative number for researchers to follow over time to assess the behavior of the injury site. Similar techniques have been described in the literature (Ford et al, 1994;Kanchiku et al, 2001). In work by Ford et al, the MRI was higher strength (1.9 T) and a special coil had been constructed.…”

Section: Discussionmentioning

confidence: 79%

“…A similar approach may be used for the spine. Other groups have also shown the utility of MRI for mouse spine evaluation, but often with higher field strength MRI machines or with specialized attachments (Ford et al, 1994;Adams, 1999;Kanchiku et al, 2001;Stroman, 2005;Biton et al, 2006;Gareis et al, 2007;Driehuys et al, 2008).…”

Section: Discussionmentioning

confidence: 97%