A method for fast determination of psoralens in oral solutions of phytomedicines using liquid chromatography

Furanocoumarins

“…This technique has been shown to be a very efficient system for the separation of this group of compounds. HPLC methods have been reported for the determination of psoralens in callus cultures, in vitro culture, serum, dermis, plants, citrus essential oils, and phytomedicines [71][72][73][74] (see Table 3). …”

Analytical Methods for Isolation, Separation and Identification of Selected Furanocoumarins in Plant Material

“…This technique has shown to be a very efficient system for separation of this group of compounds. HPLC methods have been reported for the determination of psoralens in callus cultures, vitro culture, serum, dermis, plants, citrus essential oils, phytomedicines, but only the most recently published methods has reported assay validation (Cardoso et al, 2000;Dugo et al, 2000;Markowski & Czapińska, 1997;Pires et al, 2004).…”

“…Elution was carried with acetonitrile-water 55:45 (v/v) and detections of the peaks were recording at 223 nm (Cardoso et al, 2002). The same conditions were used for determination of furanocoumarins in three oral solutions by Pires et al (2004).…”

High-performance thin-layer chromatographic analysis of psoralen in marketed formulations and manufactured solid lipid nanoparticles (SLNs): Validation of the method

“…Aforementioned, psoralens are a group of compounds that bind to DNA in the rapid cells in the presence of UV light in the A band causing DNA crosslinking and thus prevent cellular division [5][6][7]. A variety of methods has been re-ported for the analyses of psoralens including high-performance liquid chromatography [8][9][10] and gas chromatography [11]. However, to our knowledge, no validated high-performance thin-layer chromatography (HPTLC) method is available for estimation of psoralen in marketed formulation and lipid nanoparticles.…”