A method for evaluating the proportion of free and attached bacteria in the bioleaching of chalcopyrite with Thiobacillus ferrooxidans

Escobar, Beatriz; Jedlicki, Eugenia; Wiertz, Jacques V.; Vargas, T.

doi:10.1016/0304-386x(95)00005-2

Cited by 28 publications

(7 citation statements)

References 7 publications

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“…Since the direct enumeration of immobilized bacteria was not simple, an indirect method was used, i.e., using the Fe 2+ oxidation capability (Escobar et al, 1996;Pogliani and Donati, 2000). This method was based on the comparison of the oxidative capacity of inoculated jarosite with liquid inoculum, of which the bacterial concentration was known as the above-mentioned 6 × 10 7 cells mL − 1 .…”

Section: Determination Of the Number Of Viable A Ferrooxidans Cells mentioning

confidence: 99%

Simultaneous oxidation and precipitation of iron using jarosite immobilized Acidithiobacillus ferrooxidans and its relevance to acid mine drainage

Wang

Zhou

2012

Hydrometallurgy

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Section: Determination Of the Number Of Viable A Ferrooxidans Cells mentioning

confidence: 99%

Simultaneous oxidation and precipitation of iron using jarosite immobilized Acidithiobacillus ferrooxidans and its relevance to acid mine drainage

Wang

Zhou

2012

Hydrometallurgy

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“…ELIFA can also be very useful to examine how various microorganisms interact with each other and with the surfaces of mineral particles. Attached T. ferrooxidans cells were recently evaluated by measuring their ability to oxidize ferrous iron (7). But this method is based on the assumption that the specific rate of ferrous iron oxidation does not vary significantly during the bioleaching process.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-Linked Immunofiltration Assay To Estimate Attachment of Thiobacilli to Pyrite

Dziurla

Achouak

Lam

et al. 1998

Appl Environ Microbiol

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An enzyme-linked immunofiltration assay (ELIFA) has been developed in order to estimate directly and specifically Thiobacillus ferrooxidans attachment on sulfide minerals. This method derives from the enzyme-linked immunosorbent assay but is performed on filtration membranes which allow the retention of mineral particles for a subsequent immunoenzymatic reaction in microtiter plates. The polyclonal antiserum used in this study was raised againstT. ferrooxidans DSM 583 and recognized cell surface antigens present on bacteria belonging to the genusThiobacillus. This antiserum and the ELIFA allowed the direct quantification of attached bacteria with high sensitivity (104 bacteria were detected per well of the microtiter plate). The mean value of bacterial attachment has been estimated to be about 105 bacteria mg−1 of pyrite at a particle size of 56 to 65 μm. The geometric coverage ratio of pyrite by T. ferrooxidans ranged from 0.25 to 2.25%. This suggests an attachment of T. ferrooxidans on the pyrite surface to well-defined limited sites with specific electrochemical or surface properties. ELIFA was shown to be compatible with the measurement of variable levels of adhesion. Therefore, this method may be used to establish adhesion isotherms of T. ferrooxidans on various sulfide minerals exhibiting different physicochemical properties in order to understand the mechanisms of bacterial interaction with mineral surfaces.

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“…Previous research showed that A. ferrooxidans can be covered with or absorbed by iron oxyhydroxysulfate minerals during the biosynthesis of iron oxyhydroxysulfate minerals by A. ferrooxidans, which can prevent the washout of A. ferrooxidans cells in a continuously operating system. 26,27 However, Song et al reported that the total Fe precipitation efficiency (iron oxyhydroxysulfate mineral formation) was only $9% at 24 h when the density of A. ferrooxidans exceeded 5 Â 10 7 UFC mL À1 in the 9 K liquid medium with the initial pH of 2.50. 13 The results (HRT ¼ 1 d) indirectly reect the gradual decline in the cell density of A. ferrooxidans suspended in the system as a result of the continuous discharge of the effluent that eventually led to the collapse of the system.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Evaluation and optimization of a new microbial enhancement plug-flow ditch system for the pretreatment of acid mine drainage: semi-pilot test

et al. 2018

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Acid mine drainage (AMD) is typically characterized by low pH, a high concentration of sulfate and dissolved heavy metals. Therefore, it is of practical significance to promote the transformation of soluble Fe and SO 4 2À into iron hydroxysulfate minerals by biomineralization of Acidithiobacillus ferrooxidans. This enhances the lime neutralization efficiency of AMD by reducing the production of ferric hydroxide and waste gypsum. In this study, a new microbial enhanced plug-flow ditch reaction system was developed for the pretreatment of AMD on a semi-pilot scale. System stability under different hydraulic retention times (HRTs) was examined and the effects of microbe enhancement-lime neutralization technology and direct lime neutralization technology were compared. The bio-oxidation efficiency of Fe 2+ (5 g L À1 )reached 100% in some parts of the system when HRT was 3 and 2 days, and the time taken to reach steady state was 6 and 4 days, respectively. When the HRT was 1 day, the reaction system had operated for 4 days before the equilibrium was lost. At the optimum HRT (2 days) and after the system was stable, the average precipitation rate of total Fe was 53.62% and the average removal rate of As(III) was 17.27%.Following microbial enhanced pretreatment, the amount of lime required and waste residues generated for AMD neutralization decreased by 75.00% and 85.25%, respectively. This result supports the application of microbial enhancement-lime neutralization passive treatment technology for AMD.

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A method for evaluating the proportion of free and attached bacteria in the bioleaching of chalcopyrite with Thiobacillus ferrooxidans

Cited by 28 publications

References 7 publications

Simultaneous oxidation and precipitation of iron using jarosite immobilized Acidithiobacillus ferrooxidans and its relevance to acid mine drainage

Simultaneous oxidation and precipitation of iron using jarosite immobilized Acidithiobacillus ferrooxidans and its relevance to acid mine drainage

Enzyme-Linked Immunofiltration Assay To Estimate Attachment of Thiobacilli to Pyrite

Evaluation and optimization of a new microbial enhancement plug-flow ditch system for the pretreatment of acid mine drainage: semi-pilot test

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