A Method for Direct DNA Amplification of Uncharacterized DNA Viruses and for Development of a Viral Polymerase Chain Reaction Assay: Application to the Red Sea Bream Iridovirus

Oshima, Syun-ichirou; Hata, Jun-ichiro; Segawa, Chisako; Hirasawa, Noritaka; Yamashita, Shinya

doi:10.1006/abio.1996.0421

Cited by 27 publications

(20 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the amount obtained from 0.1 mg of tissue. Plasmid pRS1, which contains the segment of RSIV obtained by PCR amplification, has previously been described (Oshima et al 1996).…”

Section: Methodsmentioning

confidence: 99%

“…This method is based on the widespread presence and strong conservation of the RNRS gene among DNA viruses (Oshima et al 1996). We reported the isolation from infected fish of a 738 base pair (bp) segment of the RSIV ribonucleotide reductase small subunit gene (RNRS) (Oshima et al 1996).…”

Section: Introductionmentioning

confidence: 99%

“…We reported the isolation from infected fish of a 738 base pair (bp) segment of the RSIV ribonucleotide reductase small subunit gene (RNRS) (Oshima et al 1996). We also isolated and sequenced the endogenous RNRS gene from the red sea bream host fish (Oshima et al 1996). The host sequence was highly homologous to that of the RSIV fragment, but had sufficient nucleotide differences to enable design of PCR primers that would specifically amplify the viral segment.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction

Oshima¹,

Hata²,

Hirasawa³

et al. 1998

Dis. Aquat. Org.

View full text Add to dashboard Cite

“…the amount obtained from 0.1 mg of tissue. Plasmid pRS1, which contains the segment of RSIV obtained by PCR amplification, has previously been described (Oshima et al 1996).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction

Oshima¹,

Hata²,

Hirasawa³

et al. 1998

Dis. Aquat. Org.

View full text Add to dashboard Cite

“…Recently, the use of PCR for rapid diagnosis of RSIV prior to the onset of infection has been developed (Oshima et al, 1996;Kurita et al, 1998;Oshima et al, 1998). Although conventional PCR can detect viruses in the infected organisms (Mackay et al, 2002), it is not sufficiently sensitive for quantifying the number of viral particles (Dhar et al, 2001).…”

mentioning

confidence: 99%

Development of a Real-time PCR Assay for the Detection and Quantification of Red Seabream Iridovirus (RSIV).

Caipang¹,

Hirono²,

Aoki³

2003

Fish Pathol.

View full text Add to dashboard Cite

“…Several detection techniques have been established and applied to detect RSIV, including quantitative realtime PCR (Caipang et al, 2003), conventional PCR (Oshima et al, 1996;Oshima et al, 1998;Kurita et al, 1998), Giemsa staining (Inoue et al, 1992) and immunofluorescence assay using a monoclonal antibody (Nakajima and Sorimachi, 1995). Among these methods, real-time PCR is effective for detection and quantification of the virus.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive detection of iridovirus by loop-mediated isothermal amplification (LAMP)

Hwang¹,

Suh²,

Park³

et al. 2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Red seabream iridovirus (RSIV), a member of the Iridoviridae family, is the causative pathogen of some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of RSIV infection. In the present study, a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the sensitive and rapid detection of RSIV infection in fishes was developed. Using a set of synthesized primers matching a specific region of the RSIV genome (GenBank accession no.: AB666336.1), the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficiently detect RSIV infection in red sea bream. Our results provide a simple and convenient method for the detection of viral infection in aquatic organisms.

show abstract

A Method for Direct DNA Amplification of Uncharacterized DNA Viruses and for Development of a Viral Polymerase Chain Reaction Assay: Application to the Red Sea Bream Iridovirus

Cited by 27 publications

References 3 publications

Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction

Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction

Development of a Real-time PCR Assay for the Detection and Quantification of Red Seabream Iridovirus (RSIV).

Rapid and sensitive detection of iridovirus by loop-mediated isothermal amplification (LAMP)

Contact Info

Product

Resources

About