“…The extraction of the TC with 2-butanol is necessary to separate the TC from other fluorescent substances and from potassium ferricyanide which quenches the thiochrome fluorescence (11). The addition of the oxidizing reagent, the mixing, and the extraction with 2-butanol must be performed quickly and be carefully standardized to be reproducible (12).…”
“…The extraction of the TC with 2-butanol is necessary to separate the TC from other fluorescent substances and from potassium ferricyanide which quenches the thiochrome fluorescence (11). The addition of the oxidizing reagent, the mixing, and the extraction with 2-butanol must be performed quickly and be carefully standardized to be reproducible (12).…”
A modified fluorometric method for the determination of thiamine in food is described. The procedure is simple, reproducible, does not demand costly outfit and chemicals and is not susceptible to interference. The recovery of added thiamine is good.
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