A method for determination of free fatty acids in serum

Mikac-Dević, Dušanka; Stanković, Helena; Boškovlć, K.

doi:10.1016/0009-8981(73)90144-7

Cited by 55 publications

(10 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An advantage of this methodology is that it requires no prior protein removal, relying instead on solidphase extraction, which reduces processing time to 10 min per two samples and a GC runtime of 7 min per sample. Finally, standard methodologies were used to measure both plasma albumin (Doumas and Biggs 1972) and the nonesterified fatty acids (NEFA; Mikać-Dević et al 1973). …”

Section: Biochemical Laboratory Proceduresmentioning

confidence: 99%

Comparison of 50- and 100-g l-tryptophan depletion and loading formulations for altering 5-HT synthesis: pharmacokinetics, side effects, and mood states

et al. 2008

View full text Add to dashboard Cite

show abstract

Section: Biochemical Laboratory Proceduresmentioning

confidence: 99%

Comparison of 50- and 100-g l-tryptophan depletion and loading formulations for altering 5-HT synthesis: pharmacokinetics, side effects, and mood states

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The higher triglyceride level was then chosen in this series to ensure substrate saturation (see Results, Table I). During recirculating perfusions, 1-ml samples were withdrawn from the reservoir and the amount of free fatty acid was analyzed using the Mikac-Devic spectrophotometric method (31). The amount of free fatty acid in the medium before perfusion was subtracted and the results were corrected for the sample volumes.…”

Section: Lung Perfusion Systemmentioning

confidence: 99%

Cigarette smoke affects lipolytic activity in isolated rat lungs

Hartiala

Viikari

Hietanen

et al. 1980

Lipids

View full text Add to dashboard Cite

Isolated perfused rat lungs liberated fatty acids at a rate of 15 mumol/hr during perfusion of triglyceride-rich medium through the pulmonary vascular bed. About 80% of this activity seemed to result from lipoprotein lipase and 20% to hormone-sensitive lipase. Ventilation of the lungs with cigarette smoke instead of air during the perfusion reduced fatty acid liberation by 23%. Pre-exposure of rats to cigarette smoke for either 1 or 10 days did not cause significant changes in lung lipolytic activity compared to sham-exposed controls.

show abstract

“…Glucagon was assayed using 30K antiserum [10]. A colorometric method was used to measure non-esterified fatty acid concentrations [11]. Enzymatic methods were used to determine lactate,/3-hydroxybutyrate and glycerol concentrations [12].…”

Section: Methodsmentioning

confidence: 99%

Absence of exercise-induced hypoglycaemia in Type I (insulin-dependent) diabetic patients during maintenance of normoglycaemia by short-term, open-loop insulin infusion

et al. 1982

View full text Add to dashboard Cite

Summary. To assess the risk and possible mechanisms ofhypoglycaemia during moderate exercise in Type I (insulin-dependent) diabetic patients receiving constant insulin infusion, five insulin-dependent male diabetic patients were exercised 18 h after their last meal and 30 h after their last injection of intermediate acting insulin. Intravenous insulin was initially delivered via a closed-loop infusion system programmed to lower mean blood glucose from 11.3 _+ 1.8 to 4.8 + 0.4 mmol/1 over approximately 3.5 h. Blood glucose was then maintained at this level for 4 h. At this time, the dosed-loop infusion was discontinued and replaced by an open-loop system. The average amount of insulin infused per rain during the 4 h normoglycaemic closed-loop period was calculated and this amount was infused at a constant rate during both a 30 min period of exercise on a bicycle ergometer (approximately 65% maximum oxygen uptake) and a 30 min rest period which followed. Five nondiabetic males served as control subjects. Despite significantly higher free insulin concentrations (p < 0.05) and identical preexercise blood glucose concentrations, blood glucose rose during exercise only in the diabetic group (0.5 _+ 0.2 retool/1; p < 0.01). Changes in the serum concentrations of lactate, glycerol, glucagon, cortisol, non-esterified fatty acids and growth hormone were similar in the two groups and did not account for the increment of blood glucose in the diabetic patients. Betahydroxybutyrate concentrations were, however, higher in the diabetic patients at the onset of exercise (p < 0.01) and decreased significantly more than the control subjects during exercise. We conclude that exercise under these conditions in diabetic patients is not attended by hypoglycaemia.

show abstract

A method for determination of free fatty acids in serum

Cited by 55 publications

References 16 publications

Comparison of 50- and 100-g l-tryptophan depletion and loading formulations for altering 5-HT synthesis: pharmacokinetics, side effects, and mood states

Comparison of 50- and 100-g l-tryptophan depletion and loading formulations for altering 5-HT synthesis: pharmacokinetics, side effects, and mood states

Cigarette smoke affects lipolytic activity in isolated rat lungs

Absence of exercise-induced hypoglycaemia in Type I (insulin-dependent) diabetic patients during maintenance of normoglycaemia by short-term, open-loop insulin infusion

Contact Info

Product

Resources

About