A method for determination of 3,4-dihydroxyphenylacetic acid and 3,4-dihydroxymandelic acid in urine

Drujan, B. D.; Alvarez, Navarro N.; Borges, J. M. Díaz

doi:10.1016/0003-2697(66)90242-9

Cited by 22 publications

(4 citation statements)

References 5 publications

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“…Fluorometric procedures have been described for such urinary metabolites as 5-hydroxyindoleacetic acid, in 3.V HC1 after Sephadex separations (187), and 3,4-dihydroxyphenylacetic and 3,4dihvdroxy-mandelic acids after condensation with ethylenediamine (186). Alkoxycinchoninic acids and some related quinoline carboxylic acid derivatives show intense fluorescence which can be used for quantitation of microgram amounts in methanol or chloroform solution (108).…”

Section: Organic and Biologicalmentioning

confidence: 99%

Fluorometric analysis

White¹,

Weissler²

1968

Anal. Chem.

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Section: Organic and Biologicalmentioning

confidence: 99%

Fluorometric analysis

White¹,

Weissler²

1968

Anal. Chem.

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“…On the other hand, DOPAC is formed in human and animal organisms by the oxidative deamination of DA catalyzed by monoamine oxidase which formed during the quercetin degeneration by colonic bacteria . Determination of DOPAC has been carried out by several techniques such as liquid chromatography , fluorometry , capillary electrophoresis and HPLC . Electrochemical methods have achieved great advantages over the aforementioned techniques owing to their simplicity, rapidness and high sensitivity .…”

Section: Introductionmentioning

confidence: 99%

Fabrication of β‐Cyclodextrin/Glycine/Carbon Nanotubes Electrochemical Neurotransmitters Sensor – Application in Ultra‐sensitive Determination of DOPAC in Human Serum

et al. 2018

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In this work, an electrochemical sensor was constructed by applying two successive thin layers of glycine‐carbon nanotubes mixture and β‐cyclodextrin (CNTs‐Gly)/CD over glassy carbon electrode surface for some neurotransmitters determination. A host‐guest interaction between CD and neurotransmitters molecules is expected and resulted in enhanced sensitivity, selectivity and stability of sensor response. Other components of the sensor are crucial for the unique electrochemical response. Carbon nanotubes allowed large surface area for glycine distribution that provided hydrogen bonding to CD moieties and contributed to facilitated charge transfer. It was possible to determine 3,4‐dihydroxy phenyl acetic acid (DOPAC) in the linear range of 0.1 μmol L−1 to 80 μmol L−1 with detection limit of 9.40 nmol L−1, quantification limit of 31.5 nmol L−1 and sensitivity of 4.16 μA/μmol L−1. The proposed sensor was applied in synthetic cerebrospinal fluids samples using random standard addition method. Also, the proposed sensor was used to determine DOPAC in presence of common interferences and acceptable recovery results were achieved for its analysis in real blood serum. Figures of merit for (CNTs‐Gly)/CD composite in terms of precision, robustness, repeatability and reproducibility were reported.

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“…10 Several techniques have been used for the determination of DOPAC including electrochemical, [11][12][13][14][15][16][17][18][19] high-performance liquid chromatography, 20 chemiluminescence 21 and fluorometry. 22 Among these methods, electrochemical methods of detection * For correspondence have attracted incessantly in recent years because they are more selective, sensitive and less time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Selective determination of 3,4-dihydroxyphenylacetic acid in the presence of ascorbic and uric acids using polymer film modified electrode

Kalimuthu

John

2011

J Chem Sci

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We report here the highly sensitive and selective electrochemical determination of 3,4-dihydroxyphenylacetic acid (DOPAC), one of the dopamine metabolites in the presence of important interferents ascorbic acid (AA) and uric acid (UA) using an ultrathin electropolymerized film of 5-amino-1,3,4-thiadiazole-2-thiol (p-ATT) modified glassy carbon (GC) electrode in 0.20 M phosphate buffer solution (pH 5.0). The bare GC electrode fails to resolve the oxidation peaks of AA, DOPAC and UA in a mixture. Further, the oxidation peak potentials of them were shifted to more positive potential with decreased peak currents in the subsequent cycles. On the other hand, the p-ATT modified electrode not only separated the voltammetric signals of AA, DOPAC and UA but also enhanced their peak currents. The amperometric current response was increased linearly with increasing DOPAC concentration in the range of 4.0 × 10 −8 to 1.0 × 10 −5 M and the detection limit was found to be 150 pM (S/N = 3).

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A method for determination of 3,4-dihydroxyphenylacetic acid and 3,4-dihydroxymandelic acid in urine

Cited by 22 publications

References 5 publications

Fluorometric analysis

Fluorometric analysis

Fabrication of β‐Cyclodextrin/Glycine/Carbon Nanotubes Electrochemical Neurotransmitters Sensor – Application in Ultra‐sensitive Determination of DOPAC in Human Serum

Selective determination of 3,4-dihydroxyphenylacetic acid in the presence of ascorbic and uric acids using polymer film modified electrode

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