A method for detection of 4-hydroxy-2-nonenal adducts in proteins

Wakita, Chika; Honda, Kazumasa; Shibata, Takahiro; Akagawa, Mitsugu; Uchida, Koji

doi:10.1016/j.freeradbiomed.2011.02.037

Cited by 30 publications

(27 citation statements)

References 31 publications

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“…Furthermore, it has been reported that MDA can react with NH 2 containing amino acids and it is noteworthy that compared to amino acids, proteins have been found to be more readily modified by MDA in physiological conditions (Nair et al, 1986). Concerning hydroxylated a,b-unsaturated aldehydes, similar involvement in protein and DNA modification has been reported (Uchida and Stadtman, 1992;Uchida, 2003;Wakita et al, 2011). The LD50 of HNE for mice has been reported as 0.44 mmol kg À1 body weight if given intra-peritoneally and of HHE 0.98 mmol kg À1 of body weight (Esterbauer et al, 1991).…”

Section: Introductionmentioning

confidence: 68%

Exposure assessment of Malondialdehyde, 4-Hydroxy-2-(E)-Nonenal and 4-Hydroxy-2-(E)-Hexenal through specific foods available in Belgium

Papastergiadis

Fatouh

Jacxsens

et al. 2014

Food and Chemical Toxicology

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Section: Introductionmentioning

confidence: 68%

Exposure assessment of Malondialdehyde, 4-Hydroxy-2-(E)-Nonenal and 4-Hydroxy-2-(E)-Hexenal through specific foods available in Belgium

Papastergiadis

Fatouh

Jacxsens

et al. 2014

Food and Chemical Toxicology

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“…The relevance of 4‐HNE‐His adducts as biomarkers of OS is also supported by the fact that HNE‐His adducts dominate in Cu 2 + ‐oxidized human LDL, while the other major 4‐HNE‐derived Michael adducts (4‐HNE–lysine and 4‐HNE–cysteine) appeared in almost negligible amounts in the oxLDL, as analysed by the fluorescent probe 2‐aminopyridine labelling method recently developed by Japanese researchers (Wakita et al ., ). Thus, the specificity and applicability of 4‐HNE‐His monoclonal antibodies allows detection of the corresponding adducts in human and animal cell cultures, tissue homogenates and in plasma and serum samples, which is of primary importance for the studies on LPO end‐products in cell signalling and regulation of proliferation, differentiation, degeneration, transformation and apoptosis.…”

Section: Summary Of Methods For Detection Of Lpo: 4‐hne‐his As a Majomentioning

confidence: 97%

Biomarkers of oxidative and nitro‐oxidative stress: conventional and novel approaches

Gašparović

Žarković

et al. 2017

British J Pharmacology

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The concept of oxidative stress (OS) that connects altered redox biology with various diseases was introduced 30 years ago and has generated intensive research over the past two decades. Whereas it is now commonly accepted that macromolecule oxidation in response to ROS is associated with a variety of pathologies, the emergence of NO as a key regulator of redox signalling has led to the discovery of the pathophysiological significance of reactive nitrogen species (RNS). RNS can elicit various modifications of macromolecules and lead to nitrative or nitro-OS. In order to investigate oxidative and nitro-OS in human and in live animal models, circulating biomarker assays have been developed. This article provides an overview of key biomarkers used to assess lipid peroxidation and NO/NO 2 signalling, thereby stressing the necessity to analyse several OS biomarkers in relation to the overall (aerobic) metabolism and health condition of patients. In addition, the potential interest of heart rate variability as the non-invasive integrative biomarker of OS is discussed. LINKED ARTICLESThis article is part of a themed section on Redox Biology and Oxidative Stress in Health and Disease. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v174.12/issuetoc Abbreviations 4-HNE, trans-4-hydroxy-2-nonenal; ACR, acrolein; ALE, advanced lipoxidation end-products; DNP, 2,4-dinitrophenylhydrazine; FRR, free radical reaction; HRV, heart rate variability; LO, lipid alkoxyl radical; LOO, lipid peroxyl radical; LOOH, lipid hydroperoxide; LPO, lipid peroxidation; MDA, malondialdehyde; NO + , nitrosonium ion; Nrf2, Nuclear factor (erythroid-derived 2)-like 2; OMP, oxidatively modified protein; OS, oxidative stress; PUFA, polyunsaturated fatty acids; RNS, reactive nitrogen species; TBARS, thiobarbituric acid reactive substances; UPR, unfolded protein response IntroductionOxidative stress (OS), which was first introduced as a concept in redox biology and medicine in 1985 by Helmut Sies and Enrique Cadenas (Cadenas and Sies, 1985;Sies and Cadenas, 1985), has gained increasing interest over the years to become a major field of investigation in chemistry, life sciences and medicine.This concept connects oxidative chemistry with biological stress responses. However, it has the disadvantage of concealing redox chemistry, which plays a key role in cell homeostasis and signalling and in physiology, which is, for a large part, dependent on both oxidation and reduction reactions (Frein et al., 2005). This complex field of biochemistry, which plays a key role in the regulation of a variety of enzymes involved in essential signalling pathways, is now referred to as 'redox signalling' (Ullrich and Kissner, 2006) (Figure 1 et al., 2015). This reaction gives rise to peroxynitrite (ONOO À ), which is a highly reactive nitrogen species (RNS), producing nitration and nitrosation of substrates, often proteins that play important pathophysiological roles (Ullrich and Kissner, 2006;Schulz et al., 2008;Bottari, 20...

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“…This overabundance of chemical species greatly increases the complexity of studies attempting to characterize lipid peroxidation derived electrophilic damage. The more prominent products 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), and acrolein (ACR) have been examined under various disease models through in vitro assays, immunohistochemical staining, and analytical HPLC methods [7, 14, 15]. …”

Section: Generation Of Reactive Aldehydesmentioning

confidence: 99%

An overview of the chemistry and biology of reactive aldehydes

Fritz

Petersen

2013

Free Radical Biology and Medicine

199

215

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The non-enzymatic free radical generation of reactive aldehydes is known to contribute to diseases of sustained oxidative stress including rheumatoid arthritis, atherosclerosis, neurodegenerative and a number of liver diseases. At the same time, the accumulation of lipid electrophiles has been demonstrated to play a role in cell signaling events through modification of proteins critical for cellular homeostasis. Given the broad scope of reactivity profiles and the ability to modify numerous proteomic and genomic processes, new emphasis is being placed on a systems-based analysis of the consequences of electrophilic adduction. This review focuses on the generation and chemical reactivity of lipid-derived aldehydes with a special focus on the homeostatic responses to electrophilic stress.

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A method for detection of 4-hydroxy-2-nonenal adducts in proteins

Cited by 30 publications

References 31 publications

Exposure assessment of Malondialdehyde, 4-Hydroxy-2-(E)-Nonenal and 4-Hydroxy-2-(E)-Hexenal through specific foods available in Belgium

Exposure assessment of Malondialdehyde, 4-Hydroxy-2-(E)-Nonenal and 4-Hydroxy-2-(E)-Hexenal through specific foods available in Belgium

Biomarkers of oxidative and nitro‐oxidative stress: conventional and novel approaches

An overview of the chemistry and biology of reactive aldehydes

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