A method for automatic recording of serum lysozyme activity with the fragiligraph

Resnitzky, P; Danon, D.

doi:10.1007/bf01937797

Cited by 6 publications

(2 citation statements)

References 12 publications

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“…Leukaemic cells were separated according to density of gold grains in granules per μm 2 into: high (100–1000), medium (30–100) and low (1–20) density. Serum LZ activity was determined by a modification of a turbidometric method previously reported ( Resnitzky & Danon, 1976) (normal values 7.0 ± 2.5 μg/ml).…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural characteristics and lysozyme content in hypergranular and variant type of acute promyelocytic leukaemia

et al. 1998

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Summary.We investigated the electronmicroscopic (EM) features and cellular lysozyme (LZ) content in 16 cases of acute promyelocytic leukaemia (APL): 11 cases of the hypergranular form (M3) and five cases of the microgranular variant (M3-V). The main EM features in all cases were: irregular, folded or bilobed nuclei, many cytoplasmic granules, distended rough endoplasmic reticulum (RER) cisternae which, in some cases, presented as stellate forms (more frequent in M3-V), and bundles of cytoplasmic microfilaments. Many Auer rods were present in M3 cases and few in M3-V; most of these disclosed parallel tubular arrays (PTA) with a varied periodicity ranging from 13 to 26 nm. There was a significant difference between M3 and M3-V (P<0·0001) in both the number of granules per cell section (62·9 Ϯ 34·5 v 38·0 Ϯ 23·6) and in the granule section area (0·044Ϯ0·033 v 0·026Ϯ0·015 mm 2 ). In some cases, mainly in M3-V, we found cells with large granules containing PTA which probably represent poorly developed Auer rods. Intracellular LZ content assayed by a postembedding immunogold method, showed high granular LZ density (in the range of that found in M4 and M5) in M3 cells and very low granular LZ content in M3-V. This study adds new objective parameters for the diagnosis of these two types of APL and provides new information on their LZ pattern of expression.

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Section: Methodsmentioning

confidence: 99%

Ultrastructural characteristics and lysozyme content in hypergranular and variant type of acute promyelocytic leukaemia

et al. 1998

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“…The method used was a modification of the turbidometric method, using Micrococcus lysodeikticus (Bacto-Lysozyme Substrate-Difco) as substrate for the lysozyme and crystalline egg white lysozyme as standard enzyme (Bacto-Lysozyme-Difco), as pre viously reported [16].…”

Section: Serum Lysozyme Activity Assaymentioning

confidence: 99%

Granulopoiesis in Aged People: Inverse Correlation between Bone Marrow Cellularity and Myeloid Progenitor Cell Numbers

et al. 1987

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Results of a study on bone marrow (BM) cytology and cellularity, BM granulo-cytic-macrophage colony formation (GM-CFU-C), peripheral blood (PB) colony stimulating factor (CSF) and serum lysozyme activity in 20 elderly people aged from 64 to 89 are presented. Besides slight iron deficiency anemia, no hematological abnormality was detected. No change in PB and BM differential counts was found as compared to those obtained for normal adults. The BM cellularity and colony incidence were assessed using aliquots of the same specimen. GM-CFU-C frequency ranged from 15 to 156 (average 67.5) per 2 × 10⁵ BM cells seeded. The largest number of colonies was observed in hypocellular BM samples (p < 0.002). The level of serum lysozyme activity for the entire group was significantly higher than that obtained in healthy persons up to the age of 60 (p < 0.001). The overall results support the notion that in old age, increased proliferation of committed granulopoietic stem cells in hypoplastic areas of the BM, assure normal numbers of mature blood cells in the periphery.

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Acetylcholinesterase in Hirschsprung's Disease

Smith

Besley

Patrick³

1979

The Lancet

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A method for automatic recording of serum lysozyme activity with the fragiligraph

Cited by 6 publications

References 12 publications

Ultrastructural characteristics and lysozyme content in hypergranular and variant type of acute promyelocytic leukaemia

Ultrastructural characteristics and lysozyme content in hypergranular and variant type of acute promyelocytic leukaemia

Granulopoiesis in Aged People: Inverse Correlation between Bone Marrow Cellularity and Myeloid Progenitor Cell Numbers

Acetylcholinesterase in Hirschsprung's Disease

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