A method for 3D printing bio-cemented spatial structures using sand and urease active calcium carbonate powder

Nething, Christoph; Смирнова, М И; Gröning, Janosch A. D.; Haase, Walter; Sobek, Werner

doi:10.1016/j.matdes.2020.109032

Cited by 26 publications

(13 citation statements)

References 28 publications

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“…The developed 3DCP for construction mainly applies the additive manufacturing (AM) method by material extrusion [ 3 , 4 ]. Another 3DCP method is a powder bed-based method that performs selective binding through binder jetting [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Properties of 3D-Printed Mortar in Air vs. Underwater

et al. 2021

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Research and technological advancements in 3D concrete printing (3DCP) have led to the idea of applying it to offshore construction. The effect of gravity is reduced underwater, which can have a positive effect on 3DCP. For basic verification of this idea, this study printed and additively manufactured specimens with the same mortar mixture in air and underwater and evaluated properties in the fresh state and the hardened state. The mechanical properties were evaluated using the specimens produced by direct casting to the mold and specimens produced by extracting from the additive part through coring and cutting. The results of the experiment show that underwater 3D printing required a greater amount of printing output than in-air 3D printing for a good print quality, and buildability was improved underwater compared to that in air. In the case of the specimen layered underwater, the density and compressive strength decreased compared to the specimen layered in air. Because there are almost no effects of moisture evaporation and bleeding in water, the interlayer bond strength of the specimen printed underwater was somewhat larger than that printed in air, while there was no effect of the deposition time interval underwater.

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Section: Introductionmentioning

confidence: 99%

Comparison of Properties of 3D-Printed Mortar in Air vs. Underwater

et al. 2021

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“…Cell vitality was determined by resazurin assay according to the method of Mehring et al. [ 27 ]. For this purpose, the cell suspension was diluted to an OD of 0.2 with Sorensen buffer (pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Sporosarcina pasteurii can be used to print a layer of calcium carbonate

Erdmann

Kästner

Payrebrune

et al. 2022

Engineering in Life Sciences

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When using microbiologically induced calcium carbonate precipitation (MICP) to produce calcium carbonate crystals in the cavities between mineral particles to consolidate them, the inhomogeneous distribution of the precipitated calcium carbonate poses a problem for the production of construction materials with consistent parameters. Various approaches have been investigated in the literature to increase the homogeneity of consolidated samples. One approach can be the targeted application of ureolytic organisms by 3D printing. However, to date, this possibility has been little explored in the literature. In this study, the potential to use MICP to print calcium carbonate layers on mineral particles will be investigated. For this purpose, a dispensing unit was modified to apply both a suspension of Sporosarcina pasteurii and a calcination solution containing urea and calcium chloride onto quartz sand. The study showed that after passing through the nozzle, S. pasteurii preserved consistent cell vitality and therefore its potential of MICP. Applying cell suspension and calcination solution through a printing nozzle resulted in a layer of calcium carbonate crystals on quartz sand. This observation demonstrated the proof of concept of printing calcium carbonate by MICP through the nozzle of a dispensing unit. Furthermore, it was shown that cell suspensions of S. pasteurii can be stored at 4°C for a period of 17 days while maintaining its optical density, urease activity and cell vitality and therefore the potential for MICP. This initial concept could be extended in further research to printing three‐dimensional (3D) objects to solve the problem of homogeneity in consolidated mineral particles.

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“…This demonstrates the ability of the EICP to be used for soil improvement in the field using conventional installation methods. Finally, Nething et al [124] successfully implemented biomineralization in 3D printing a rigid and stable bio-cemented sand structure using urease active calcium carbonate powder.…”

Section: Envisioned Applications Of Soil Bio-cementation Via Eicpmentioning

confidence: 99%

State-of-the-Art Review of Enzyme-Induced Calcite Precipitation (EICP) for Ground Improvement: Applications and Prospects

et al. 2021

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The global construction industry consumes huge amounts of mined materials that are considered unsustainable for earth resources. In addition, Portland cement which is a key element in concrete and most construction materials is considered one of the main contributors to worldwide CO2 emissions. On the other hand, natural cemented soil deposits are examples of sustainable structures that have survived decades of severe environmental conditions. Mimicking these natural biological systems provide an alternative to the current practices of construction materials production. Enzyme-induced carbonate precipitation (EICP) is a bio-inspired technique based on the precipitation of calcium carbonate for enhancing the geo-mechanical properties of soils. In this technique, calcium carbonate acts as a cementitious agent that binds the soil particles together at the points of contact, hence, increasing the strength and stiffness of treated soils, while relatively reducing the soil permeability and porosity. The achieved enhancements make EICP useful for applications such as ground improvement, construction materials, and erosion control over traditional binders. This paper presents a state-of-the-art review of EICP for ground improvement including the fundamental basics of EICP treatment. The paper also discusses the chemical and physical factors affecting the performance of EICP such as enzyme source, enzyme activity and solution constitutes. Moreover, the paper reviews the different methods and testing techniques used in the application of EICP for soil treatment. Furthermore, the paper compares EICP with other biomineralization techniques in terms of performance and applicability on ground improvement. Finally, the paper discusses the research gaps and existing challenges concerning the commercialization and large-scale implementation of the technology.

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A method for 3D printing bio-cemented spatial structures using sand and urease active calcium carbonate powder

Cited by 26 publications

References 28 publications

Comparison of Properties of 3D-Printed Mortar in Air vs. Underwater

Comparison of Properties of 3D-Printed Mortar in Air vs. Underwater

Sporosarcina pasteurii can be used to print a layer of calcium carbonate

State-of-the-Art Review of Enzyme-Induced Calcite Precipitation (EICP) for Ground Improvement: Applications and Prospects

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