A method based on 1H NMR spectral data useful to evaluate the hydrolysis level in complex lipid mixtures

Nieva‐Echevarría, Bárbara; Goicoechea, Encarnación; Manzanos, María J.; Guillén, Marı́a D.

doi:10.1016/j.foodres.2014.09.031

Cited by 129 publications

(95 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Table 1 shows the assignment of the 1 H NMR signals from the spectra of the samples studied, as shown in Figs. 1 and 2; this assignment is in agreement with previous studies (Guillén & Ruiz, 2001, 2003aGuillén et al, 2008;Nieva-Echevarría et al, 2014;Vidal et al, 2012).…”

Section: H Nmr Spectra Acquisitionsupporting

confidence: 93%

“…As in previous studies of edible oils carried out in our laboratory (Guillén, Carton, Goicoechea, & Uriarte, 2008;Martínez-Yusta & Guillén, 2014;Nieva-Echevarría et al, 2014;Vidal, Manzanos, Goicoechea, & Guillén, 2012), 200 ll of the lipid samples were mixed with 400 ll deuterated chloroform (CDCl 3 ), which contains 0.2% of non-deuterated chloroform and a small proportion of tetramethylsilane (TMS) used as internal reference (Cortec, Paris, France). The mixture was introduced into a 5 mm diameter tube.…”

Section: H Nmr Spectra Acquisitionmentioning

confidence: 99%

“…Given this, the number of moles of the different kinds of molecules present in the lipid samples can be calculated by the following equations, developed and validated in a previous study (Nieva-Echevarría et al, 2014):…”

Section: Equations Derived From 1 H Nmr Spectral Data Used To Quantifmentioning

confidence: 99%

“…More recently, a new approach based on Proton Nuclear Magnetic Resonance ( 1 H NMR) has been proposed to study qualitatively and quantitatively all the lipolytic products arising from TG hydrolysis (Nieva-Echevarría, Goicoechea, Manzanos, & Guillén, 2014). Due to the proportionality existing between the area of the 1 H NMR spectral signals and the number of protons that generate them, different equations were developed and validated to calculate the molar percentages of TG, DG, MG and FA in complex mixtures of standard compounds simulating lipid hydrolysates from vegetable, terrestrial animal and marine origins.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Usefulness of 1H NMR in assessing the extent of lipid digestion

et al. 2015

Self Cite

View full text Add to dashboard Cite

Proton Nuclear Magnetic Resonance ((1)H NMR) is proved to be, for the first time, a very useful technique in monitoring the extent of lipid hydrolysis in digestion processes. Sunflower oil and minced fish flesh, as model foods, were subjected to different in vitro digestion experiments and the lipolysis levels reached were evaluated using (1)H NMR spectral data. Simple observation of the spectra gives very valuable information about the extent of the lipolysis and enables a rapid discrimination among samples having different hydrolysis degree. Equations were developed to quantify all the lipolytic products, and either referred to acyl groups plus fatty acids, or to glyceryl structures. The main hydrolysis products were 1,2-diglycerides, 2-monoglycerides, glycerol and fatty acids, although small proportions of 1,3-diglycerides and of 1-monoglycerides were also found. With this methodology, determination of the extent of lipid digestion in its different definitions can be made. It has been shown that these definitions are not equivalent, which is evidence for the need for a consensus in this regard.

show abstract

Section: H Nmr Spectra Acquisitionsupporting

confidence: 93%

Section: H Nmr Spectra Acquisitionmentioning

confidence: 99%

Section: Equations Derived From 1 H Nmr Spectral Data Used To Quantifmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Usefulness of 1H NMR in assessing the extent of lipid digestion

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several specific methods have been described to determine these compounds in edible oils (Liu, Lee, Bobik, Guzman-Harty, & Hastilow, 1993;Schoenfelder, 2003). They can even be determined by 1 H NMR, if they are in high enough concentrations (Nieva-Echevarría, Goicoechea, Manzanos, & Guillén, 2014). The monoglycerides found in the oils here studied are given in Table 3.…”

Section: Monoglyceridesmentioning

confidence: 99%

A new methodology capable of characterizing most volatile and less volatile minor edible oils components in a single chromatographic run without solvents or reagents. Detection of new components

et al. 2017

Self Cite

View full text Add to dashboard Cite

Quality Control in Foods, State of the Art and Prospects

Reyes

García

2016

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Quality control (QC) of food includes control of authenticity, origin, properties or attributes, composition, and safety involving all steps of the global supply chain – from the raw material up to the final product sold on the supermarket shelf. Despite the great concern regarding their quality, these products are currently monitored and certified by means of paper documentation, some targeted analysis, and sensory properties. This article encompasses a revision on the instrumental analytical techniques, methods, and systems used in food QC together with their main applications and a brief historical perspective on food analysis, together with a deep revision on the current state of art of modern analytical instruments, methodologies, and applications in the QC of food in order to establish Quo Vadis QC of foods. This article also discusses the present and future challenges in QC of food, the need for faster techniques, the prospect of advanced imaging techniques, and the application of ‘omic’ in food QC (including metabolomics, proteomics, etc.)

show abstract

A method based on 1H NMR spectral data useful to evaluate the hydrolysis level in complex lipid mixtures

Cited by 129 publications

References 31 publications

Usefulness of 1H NMR in assessing the extent of lipid digestion

Usefulness of 1H NMR in assessing the extent of lipid digestion

A new methodology capable of characterizing most volatile and less volatile minor edible oils components in a single chromatographic run without solvents or reagents. Detection of new components

Quality Control in Foods, State of the Art and Prospects

Contact Info

Product

Resources

About