A methed for the study and filming of ciliary activity among animals and human beings

Frenckner, Paul; Richtnér, Nils G.

doi:10.3109/00016483909123749

Cited by 13 publications

(3 citation statements)

References 23 publications

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“…Wave-like movements have been accepted by numerous workers as visible manifestations of ciliary beat (23)(24)(25)(26)(27)(28)17) and have frequently been used as a test of whether application of drugs to respiratory epithelia does harm to the ciliary mechanism. In view of this general opinion, it is necessary to realize that such movements, as observed in the bronchus of the mouse, were not found to be due to cilia.…”

Section: Discussionmentioning

confidence: 99%

“…In order to ascertain the effect of viral infection on broncial cilia, it was necessary to have a method for evaluation of ciliary action. A technique frequently used for this purpose consists of the visualization of wave-like movements on the surface of respiratory epithelium (23)(24)(25)(26)(27)(28)17), and adaptation of this method to the bronchus of the mouse was readily made.…”

Section: Wave-like Movements Of the Bronch~ ~mentioning

confidence: 99%

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Effect of Influenza Virus on Cilia and Epithelial Cells in the Bronchi of Mice

Harford¹,

Hamlin²

1952

Journal of Experimental Medicine

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A study of bronchial cilia in mice was carried out in order to obtain evidence as to how influenza viral infection of the mouse lung lowers rcsistancc to secondary pneumococcal pneumonia. Earlier work had shown that this effect is associated with the viral lesion rather than the presence of the virus per se and had suggested that viral infection damages antibacterial mechanisms of the lung (I). Particular attention was given to cilia because of the histologic evidence that influenza viral infection of the mouse causes necrosis of the bronchial epithelium (I-8). In addition, it has bccn shown that ciliary action removes foreign particles from the respiratory passages and therefore probably serves as an important mechanism of nativc resistance to bacterial infection of the respiratory tract (9-20). I When severe infection with influenza virus was induced in mice, it was found that active ciliary beat persisted in the bronchi of involved lungs and that cilia could be shown to be present in stained sections of the viral lesion. = These findings were unexpected because it was thought that this viral infection causes destruction of bronchial epithelial cells. For this reason, further experiments were done to clarify the nature of the viral lesion. The result of this investigation has revealed no evidence that actual destruction of the epithelium takes place. Instead of nuclear changes in the early viral lesion, basophilic intracytoplasmic inclusion bodies have bccn found. Evidence has also been obtained to indicate that portions of the cytoplasm of non-ciliated cells become detached and are shed into the lumen. EXPERIMENTAL METHODS AND RESULTS Wave-Like Movements of the Bronch~ ~In order to ascertain the effect of viral infection on broncial cilia, it was necessary to have a method for evaluation of ciliary action. A technique frequently

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Section: Discussionmentioning

confidence: 99%

Section: Wave-like Movements Of the Bronch~ ~mentioning

confidence: 99%

Effect of Influenza Virus on Cilia and Epithelial Cells in the Bronchi of Mice

Harford¹,

Hamlin²

1952

Journal of Experimental Medicine

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“…Ciliary movement has been measured by the following methods: a) the particle transport method (1), b) the photographic method by which the frequency of the ciliary beat can be recorded directly using a stroboscope (2,3), c) the photoelectric method by which frequency of voltage variations corresponding to the ciliary beat frequency is recorded on an oscilloscope or is counted electrically in a rate meter (4). These methods have been used to examine the effect of chemical compounds on ciliary activity in the mucus-covered system of a variety of experimental models (5)(6)(7)(8).…”

mentioning

confidence: 99%